LI Li,HE Jianmin,LIU Yan,et al.Optimization of Microwave-assisted Extraction of Laccaria laccata Polysaccharides by Response Surface Method and Its Antioxidant-activities[J].Northern Horticulture,2018,42(15):133-139.[doi:10.11937/bfyy.20174479]
响应面法优化微波辅助提取保山红蜡蘑多糖及其抗氧化活性研究
- Title:
- Optimization of Microwave-assisted Extraction of Laccaria laccata Polysaccharides by Response Surface Method and Its Antioxidant-activities
- 文献标志码:
- A
- 摘要:
- 以保山野生红蜡蘑为试材，采用微波辅助法提取了红蜡蘑多糖，在单因素试验的基础上，采用Box-Behnken响应面法对多糖提取的显著因素:微波时间、微波功率、液料比进行了优化；用对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和羟自由基(·OH)的清除率测定红蜡蘑多糖的抗氧化活性。结果表明：多糖提取的最佳工艺为微波时间90 s，微波功率350 W，液料比50∶1 mL·g-1，水浴温度80 ℃，水浴时间2 h，多糖平均得率为6.75%，与预测值（6.79%）相比，相对误差较小，为0.59%；红蜡蘑多糖对DPPH·和·OH具有明显的清除能力，红蜡蘑多糖是一种具有抗氧化能力的食用真菌多糖，值得进一步重视和研究。
- Abstract:
- The wild Laccaria laccata in Baoshan was used as the material,the polysaccharides was extracted by microwave assisted method,based on the single factor experiment,the response surface method of Box-Behnken significant factors of microwave time,microwave power and liquid-solid ratio.The antioxidation of polysaccharides was determined by the scavenging effect of DPPH· and ·OH.The results showed that the optimum conditions of Laccaria laccata polysaccharides were：microwave time 90 seconds,microwave power 350 W,liquid-solid ratio 50∶1 mL·g-1,water bath temperature 80 ℃,water bath time 2 hours,and the polysaccharide yield was 6.75%.Compared with predicted value (6.79%),it had a smaller relative error at 0.59%.Furthermore,Laccaria laccata polysaccharides had remarkable scavenging activity to DPPH· and ·OH,which indicated that was a strong natural antioxidation and further study was worth doing.
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备注/Memo
第一作者简介：李丽（1982-），女，硕士，副教授，研究方向为天然产物提取。E-mail:18660940@qq.com.基金项目：2015年地方高校国家级大学生创新创业训练计划资助项目（201510686002）；保山科技局2016年市级科技计划资助项目（2016bs101）。收稿日期：2018-03-05