[1]大白菜LOG基因家族的鉴定与生物信息学分析.大白菜LOG基因家族的鉴定与生物信息学分析[J].北方园艺,2018,42(04):18-26.[doi:10.11937/bfyy.20173153]
　HUO Ruxue,SUN Lin,LIU Zhenning.Identification and Bioinformatics Analysis of LOG Gene Family in Chinese Cabbage[J].Northern Horticulture,2018,42(04):18-26.[doi:10.11937/bfyy.20173153]
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大白菜LOG基因家族的鉴定与生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年04 页码: 18-26 栏目: 出版日期: 2018-02-28
Title:
Identification and Bioinformatics Analysis of LOG Gene Family in Chinese Cabbage
作者:
大白菜LOG基因家族的鉴定与生物信息学分析
霍如雪1，孙琳1，刘振宁1,2
Author(s):
HUO Ruxue1; SUN Lin1; LIU Zhenning1; 2
1.College of Agriculture and Forestry Sciences,Linyi University,Linyi,Shandong 256000；2.College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,Zhejiang 310058
关键词:
大白菜; LOG基因家族; 生物信息学
Keywords:
Chinese cabbage(Brassica rapa); LOG gene family; bioinformatic
DOI:
10.11937/bfyy.20173153
文献标志码:
A
摘要:
LOG基因编码5′-核糖单磷酸水解酶，将细胞分裂素的前体物质直接转化成具有生理活性的细胞分裂素，在细胞分裂素合成途径中具有重要作用。为深入研究大白菜LOG基因家族的功能，利用生物信息学方法对大白菜基因组中LOG基因家族成员进行了鉴定，并对其基因组信息、蛋白质生理生化特征、基因结构、保守结构域、系统进化等方面进行了研究。结果表明：在大白菜基因组中共鉴定出17个LOG基因，可以聚类到7个分支A~G中；LOG基因在大白菜10条染色体上呈现不均匀分布，在第2和4 号染色体上分布相对较多；对大白菜LOG蛋白氨基酸序列进行了多重比对，比对结果表明LOG蛋白的氨基酸中均具有一致序列为MHxRKAxMx（5)FxALPGGYGTxxEE的LOG蛋白特异的LDC motif；通过MEME〖JP+2〗软件对大白菜LOG蛋白motif的预测发现了8个比较保守的motif。研究结果为大白菜LOG基因功能的研究奠定了基础。关键词：大白菜；LOG基因家族；生物信息学
Abstract:
LOG genes encode riboside 5′-monophosphate enzymes,which could directly transform cytokinin precursors into cytokinins with physiological activities,thus acting essential roles in the biosynthetic pathways of cytokinins.To further research the LOG gene family functions of Chinese cabbage,bioinformatics tools were conducted to identify the LOG gene family members in the Chinese cabbage genome,and genome annotation,physiological and biochemical characteristics of proteins,gene structures,conserved domains and phylogenetic trees were also analyzed.The results showed that 17 LOG genes were identified in the whole genome level,which could be clustered into 7 clades,A-G；LOG genes were unevenly distributed in the 10 chromosomes,among which chromosome 2 and chromosome 4 contained relatively more LOG genes.Multiple alignment of LOG protein sequences were conducted and results indicated the consensus sequences of MHxRKAxMx（5)FxALPGGYGTxxEE,which was specific to LDC motif in LOG proteins.Besides,8 conserved motifs were predicted among LOG proteins by MEME analysis.These results established certain foundation for LOG gene functions study in Chinese cabbage.

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备注/Memo

第一作者简介：霍如雪（1988-），女，硕士，讲师，研究方向为蔬菜分子生物学。E-mail:huoruxue@lyu.edu.cn.

责任作者：刘振宁（1988-），男，博士，讲师，研究方向为蔬菜分子生物学与遗传育种。E-mail：liuzhenning@lyu.edu.cn.
基金项目：〖JP3〗山东省自然科学基金资助项目（ZR2017PC012）；〖JP〗国家自然科学基金资助项目（31700272）。
收稿日期：2017-10-30

更新日期/Last Update: 2018-02-23