[1]郭康迪,郝学政,王亚军,等.河南省苦瓜枯萎病菌的分离鉴定[J].北方园艺,2018,42(05):47-53.[doi:10.11937/bfyy.20173125]
　GUO Kangdi,HAO Xuezheng,WANG Yajun,et al.Isolation and Identification of Fusarium Species From  Wilted Bitter Gourd in Henan Province[J].Northern Horticulture,2018,42(05):47-53.[doi:10.11937/bfyy.20173125]
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河南省苦瓜枯萎病菌的分离鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年05 页码: 47-53 栏目: 出版日期: 2018-03-15
Title:
Isolation and Identification of Fusarium Species From  Wilted Bitter Gourd in Henan Province
作者:
郭康迪1; 郝学政2; 王亚军1; 文才艺1
（1.河南农业大学 植物保护学院，河南 郑州 450002； 2.河南省农业厅 中药材生产技术服务中心，河南 郑州 450002）
Author(s):
GUO Kangdi1; HAO Xuezheng2; WANG Yajun1; WEN Caiyi1
(1.College of Plant Protection，Henan Agricultural University，Zhengzhou，Henan 450002；2.The Service Center of Production Technology of Chinese Medicinal Materials，Department of Agriculture of Henan Province，Zhengzhou，Henan 450002)
关键词:
苦瓜; 尖孢镰孢菌; 分类鉴定; 致病性测定
Keywords:
bitter gourd; Fusarium oxysporum; classification and identification; pathogenicity assay
DOI:
10.11937/bfyy.20173125
文献标志码:
A
摘要:
以河南省郑州、周口和信阳的6个苦瓜种植园区的苦瓜枯萎病样品为试材，采用组织分离法分离苦瓜枯萎病菌分离物，并根据分离物的培养性状、菌落及孢子形态特征，结合分子系统学分析和致病性测定，对苦瓜枯萎病菌进行了分类鉴定，以期明确河南省苦瓜枯萎病菌分离物的分类地位，并为苦瓜枯萎病综合防治提供参考依据。结果表明：34株分离物均为苦瓜枯萎病菌尖孢镰孢菌苦瓜专化型(Fusarium oxysporum f.sp.momodicae Sun ＆ Huang)。分离物除了侵染苦瓜外，弱侵染丝瓜和瓠瓜，不侵染南瓜、甜瓜、西瓜和黄瓜。基于〖STBX〗ITS、EF-1α〖STBZ〗和β-tubulin的多基因序列系统发育树的分析结果表明，来源于郑州和信阳的苦瓜枯萎病菌与以上瓜类专化型的亲缘关系较近，除郑州分离物HeN2外，所有郑州和信阳分离物均与丝瓜专化型、西瓜专化型、甜瓜专化型聚类于同一个亚群。周口分离物可明显的分为2~3个亚群，其中，HeN25、HeN27、HeN28、HeN30和HeN32为单独的亚群，其亲缘关系与黄瓜专化型较近；HeN18、HeN26、HeN29、HeN31、HeN33和HeN34与香蕉专化型的亲缘关系较近。
Abstract:
Fusarium species were obtained from wilted bitter gourd samples collected from six bitter gourd fields of Zhengzhou，Zhoukou and Xinyang in Henan Province using the method of tissue isolation.In order to clarify the classification status of the Fusarium species in Henan Province and provide a reference for Fusarium wilt prevention.Isolates were identified via the culture features，the morphological characteristics of colony and spore，molecular phylogenetic analysis and pathogenicity.The results showed that all isolates were identified as Fusarium oxysporum f.sp.momodicae Sun ＆ Huang.These isolates could infect the seedlings of bitter gourd，and luffa，bottle gourd weakly，but not pumpkin，muskmelon，watermelon and cucumber.Phylogenetic tree analysis based on multiple gene sequences of ITS，[STBX]EF-1α[STBZ] and β-tubulin showed that the strains of Fusarium oxysporum from Zhengzhou and Xinyang had closer genetic relationship with the above specialization.Except HeN2 from Zhengzhou，all isolates from Zhengzhou and Xinyang were clustered to the same subgroup with Fusarium oxysporum f.sp.lagenariae，Fusarium oxysporum f.sp.niveum，and Fusarium oxysporum f.sp.melonis.The isolates from Zhoukou could be significantly divided into 2-3 subgroups，of which，HeN25，HeN27，HeN28，HeN30 and HeN32 belonged to the same subgroup and had closer genetic relationship with Fusarium oxysporum f.sp.cucumerinum.HeN18，HeN26，HeN29，HeN31，HeN33 and HeN34 had closer genetic relationship with Fusarium oxysporum f.sp.cubense

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备注/Memo

第一作者简介：郭康迪（1992-），女，硕士研究生，〖JP2〗研究方向为植物病害生物防治。E-mail：guokangdi0316@163.com. 责任作者：文才艺（1965-），男，博士，教授，研究方向为植物病害生物防治。E-mail：13683800681@163.com. 基金项目：公益性行业（农业）科研专项资助项目（201503110）。收稿日期：2017-10-12

更新日期/Last Update: 2018-03-15