[1]张文波,白杨,余利敏,等.大兴安岭野生笃斯越桔逆转座子逆转录酶的多样性分析[J].北方园艺,2018,42(04):41-48.[doi:10.11937/bfyy.20172738]
　ZHANG Wenbo,BAI Yang,YU Limin,et al.Diversity Analysis of Retrotransposon Reverse Transposases From Wild Blueberry Vaccinium uliginosum L.in Greater Khingan[J].Northern Horticulture,2018,42(04):41-48.[doi:10.11937/bfyy.20172738]
点击复制

大兴安岭野生笃斯越桔逆转座子逆转录酶的多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年04 页码: 41-48 栏目: 出版日期: 2018-02-28
Title:
Diversity Analysis of Retrotransposon Reverse Transposases From Wild Blueberry Vaccinium uliginosum L.in Greater Khingan
作者:
张文波; 白杨; 余利敏; 伏鸿峰; 闫伟
内蒙古农业大学 林学院，内蒙古 呼和浩特 010019
Author(s):
ZHANG Wenbo; BAI Yang; YU Limin; FU Hongfeng; YAN Wei
College of Forestry,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019
关键词:
笃斯越桔; 逆转座子; 逆转录酶; 遗传多样性
Keywords:
Vaccinium uliginosum; reverse transposase; retrotransposon; genetic diversity
DOI:
10.11937/bfyy.20172738
文献标志码:
A
摘要:
以大兴安岭野生笃斯越桔为试材，采用同源克隆技术分离了Ty3-Gypsy类逆转座子逆转录酶序列，研究了其同源性及系统进化关系，以期为分析笃斯越桔的遗传多样性，开发可靠的分子标记提供参考依据。结果表明：分离的19条序列（分别命名为VuGRE1~19）长度范围为376~418 bp，同源性范围为23.3%~97.8%，显示出较高的异质性。氨基酸序列分析结果显示，分离的19条序列中有8条序列发生了不同程度的变异，其中终止密码子突变的频率最高；因此推测终止密码子突变可能是导致笃斯越桔逆转座子异质性的主要原因。系统进化分析结果显示，VuGRE1~19隶属于2个不同的基因家族。Family I仅包含3条序列，序列间的同源性较低，且与其它物种逆转录酶的遗传距离较远；说明Family I的起源较为古老，种的特异性较强。Family II包含16条序列，由3个Subfamily组成；各亚家族成员间的同源性较高，亲缘关系较近；说明该基因家族的活性较高。另外，Family II基因家族成员与其它物种的Ty3-Gypsy类逆转座子逆转录酶序列具有较高的同源性，特别是Subfamily 3与兴安落叶松、苹果、银杏、绿豆、荸荠等植物的逆转录酶具有较近的亲缘关系；说明这些成员在物种间存在广泛的横向交流。以上结果说明，Family II是笃斯越桔中广泛存在的逆转座子基因家族，并在笃斯越桔适应性遗传进化中发挥了重要作用。
Abstract:
For analyzing genetic diversity and empoldering credible molecular marker of wild blueberry, Vaccinium uliginosum in Greater Khingan,reverse transposases of Ty3-Gypsy-like retrotransposons were isolated using homology based cloning techniques,and homology and phylogenetic relation were analyzed.The results revealed that the length of the nucleotide sequences of the 19 isolated sequences （named as VuGRE1-19) varied from 376 bp to 418 bp with homology ranged from 19.8%-97.8%,showing a high heterogeneity.Sequence analysis for the VuGRE1-19 showed that the stop code mutation with a high frequency occured in eight sequences,suggesting terminator mutation was the major reason resulted in heterogeneity of retrotransposons in V.uliginosum.Phylogenetic analysis showed that the 19 of sequences are consisted of two families,Family I contained only 3 sequences which showed low homology between each other,and far relationships with reverse transposases from the other plants,revealing ancient origin and low transcriptional activity.Family II contained 16 sequences,which consisted of 3 Subfamilies,the sequences of the Subfamilies show high similarity and close relationships between each other.The sequences of Subfamily 3 had a closer relationship with reverse transposases from the other plants,such as Malus pumila,Larix gmelinii,Peperomia caperata,Vigna radiate,Prunus salicina and Eleocharis dulcis,indicating extensive transfer of the subfamily members among different plant species.These results suggested that the Family II retrotransposons widespreadly existed in genome and played an important role in adaptive genetic evolution of the V.uliginosum.

参考文献/References:

[1]马毓泉.内蒙古植物志[M].内蒙古:内蒙古人民出版社,1992:12.

[2]周繇.长白山区野生珍稀濒危药用植物资源评价体系的初步研究[J].西北植物学报,2006,26（3)：599-605.
[3]周繇.长白山区珍稀濒危植物优先保护序列的研究[J].林业科学研究,2006,19（6)：740-749.
[4]刘庆忠,赵红军,郑亚芹,等.高灌蓝莓微体繁殖技术研究初报[J].落叶果树,2001（5)：1-3.
[5]刘庆忠,赵红军.高灌蓝莓的组织培养及快速繁殖[J].植物生理学通讯,2002,38（3)：253.
[6]刘世彪,陈功锡.武陵山地区野生果树种质资源及其开发利用[J].果树学报,2002（19)：399-405.
[7]王辉,王鹏云,王蜀,等.我国蓝莓的发展现状及前景[J].农业现代化研究,2008,29（2)：389-392.
[8]KUMAR A,BENNETZEN J L.Plant retrotransposons[J].Annual Review of Genetics,1999,33（1)：479-532．
[9]路铁刚,孙敬三.植物逆转座子及其在基因功能和基因组分析中的应用[J].遗传,1997,19（4)：39-44.
[10]DEVOS K M,BROWN J K M,BENNETZEN J L.Genome size reduction through illegitimate recombination counteracts genome expansion in Arabidopsis[J].Genome Res,2002（12):1069-1075.
[11]TANG Y,MA Y.Characteristics of plant retrotransposons and their application in functional genomics[J].Plant Genetic Res,2005（2):221-225.
[12]BENNETZEN J L.The contributions of retroelements to plant genome organization,function and evolution[J].Trends Microbiol,1996（4)：347-353.
[13]KUMAR A.The adventures of the Ty1-copia group of retrotransposons in plants[J].Trends Genet,1996,12（2)：41-43.
[14]GRANDBASTIEN M A,SPIELMANN A,CABOCHE M.Tnt1,a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics[J].Nature,1989,337（6205):377-380.
[15]VOYTAS D F,CUMMINGS M P,KONICZNY A,et al.Copia-like retrotransposon are ubiquitous among plants[J].Proc Natl Acad Sci,1992,89（15)：7124-7128.
[16]SUONIEMI A,TANSKANEN J,SCHULMAN A H.Gypsy-like retrotransposons are widespread in the plant kingdom[J].Plant,1998（13)：699-705.
[17]CHANG S,PURYEAR T,CAIMEY I.A simple and efficient method for isolating RNA from pine trees[J].Plant Mol Bio Rep,1993（11):113-116.
[18]SCHULMAN A H.Molecular markers to assess genetic diversity[J].Euphytica,2007,158：313-321．
[19]DOOLITTLE R F,FENG D F,JOHNSON M S,et al.Origins and evolutionary relationships of retroviruses[J].Q Rev Biol,1989,64：1-30.
[20]SAITOU N,NEI M.The neighbor-joining method：A new method for reconstructing phylogenetic trees[J].Mol Biol Evol,1987（4):406-425.
[21]STERGIOU G,KATSIOTIS A,HAGIDIMITRIOU M,et al.Genomic and chromosomal organization of Ty1-copia-like sequences in Olea europaea and evolutionary relationships of Olea retroelements[J].Theor Appl Genet,2002,104（6-7):926-933.
[22]APT K E,GROSSMAN A R.Characterization and transcript analysis of the majorphycobiliprote in subunit genes from Aglaothamnion neglectum（Rhodophyta)[J].Plant Mol Biol,1993（21):27-38．
[23]SUONIEMI A,TANSKANEN J,SCHULMAN A H.Gypsy-like retrotransposons are widespread in the plant kingdom[J].Plant,1998（13)：699-705.
[24]STEINHAUER D A,HOLLAND J J.Direct method for quantitation of extreme polymerase error frequencies at selected single base sites in viral RNA[J].J Virol,1986,57（1)：219-228.
[25]GRANDBASTIEN M A.The expression of the tobacco Tntl retrotransposon is linked to the plant defense responses[J].Genetica,1997,100（1-3):241-252.
[26]KUMAR A.The evolution of plant retroviruses：Moving to green pasture[J].Trends Plant Sci,1998（3)：371-374.
[27]PEARCE S R,KUMAR A,FLAVELL A J.Activation of the Ty1-copia group retrotansposons of potato （Solanum tuberosum) during protoplast isolation[J].Plant Cell Reports,1996（15)：949-953.
[28]ROGERS S A,PAULS K P.Ty1-copia-like retrotransposons of tomato （Lycopersicon esculentum Mill.)[J].Genome,2000,43：887-894.

相似文献/References:

[1]李根柱,陈英敏,王贺新,等.长白山东坡落叶松林下笃斯越桔群落的生长特征调查[J].北方园艺,2013,37(14):101.
　LI Gen-zhu,CHEN Ying-min,WANG He-xin,et al.Investigation of Growth Characteristics of Vaccinium uliginosum Community Under Larix olgensis Forest in Eastern Slopes of Changbai Mountain[J].Northern Horticulture,2013,37(04):101.
[2]宗长玲,宗成文,赵巍巍,等.长白山笃斯越桔优良单株组培快繁技术的研究[J].北方园艺,2012,36(04):113.
　ZONG Chang-ling,ZONG Cheng-wen,ZHAO Wei-wei,et al.In vitro Culture of Superior Individual of Vaccinium uliginosum from Changbai Mountain[J].Northern Horticulture,2012,36(04):113.
[3]黄磊,姜国斌,王贺新,等.全球变化对北半球高纬度地区主要越桔属物种的影响[J].北方园艺,2014,38(22):190.
　HUANG Lei,JIANG Guo-bin,WANG He-xin,et al.Effect of Global Change on the Species of Vaccinium in High Latitudes of the Northern Hemisphere[J].Northern Horticulture,2014,38(04):190.
[4]宗 长 玲,邓 　 萌,宗 成 文,等.笃斯越桔研究进展[J].北方园艺,2011,35(12):0.[doi:10.11937/bfyy.2011012064]
　ZONG Chang-ling,DENG Meng,ZONG Cheng-wen,et al.Research Progress on Vaccinium uliginosum Linn[J].Northern Horticulture,2011,35(04):0.[doi:10.11937/bfyy.2011012064]
[5]李悦怡,宋春艳,谭智文,等.笃斯越桔苗期合理施肥研究[J].北方园艺,2016,40(21):12.[doi:10.11937/bfyy.201621003]
　LI Yueyi,SONG Chunyan,TAN Zhiwen,et al.Fertilization Research of Vaccinium uliginosum Linn. Seedling[J].Northern Horticulture,2016,40(04):12.[doi:10.11937/bfyy.201621003]
[6]李冰,苏上,王丽金,等.笃斯越桔高效再生体系的建立[J].北方园艺,2017,41(11):124.[doi:10.11937/bfyy.201711025]
　LI Bing,SU Shang,WANG Lijin,et al.Efficient Regeneration System for Vaccinium uliginosum L.[J].Northern Horticulture,2017,41(04):124.[doi:10.11937/bfyy.201711025]
[7]郭雪,李兴国,孙侨冶,等.响应面试验优化加压水提法提取笃斯越桔多糖的工艺研究[J].北方园艺,2019,43(05):116.[doi:10.11937/bfyy.20182804]
　GUO Xue,LI Xingguo,SUN Qiaoye,et al.Optimization of Pressurised Water Extraction of Polysaccharides From Vaccinium uliginosum L. Using Response Surface Methology[J].Northern Horticulture,2019,43(04):116.[doi:10.11937/bfyy.20182804]

备注/Memo

第一作者简介：张文波（1971-)，男，博士，副教授，研究方向为林木遗传育种。E-mail：wenbo20090101@163.com.

基金项目：国家自然科学基金资助项目（31660213,31560065,31160143,31260168，31060106）；内蒙古自然科学基金资助项目（2015MS0354）；内蒙古农业大学博士基金资助项目（BJ08-27）。
收稿日期：2017-10-30

更新日期/Last Update: 2018-02-24