[1]李泽宏,袁红慧,程华,等.银杏GbERF1转录因子基因的克隆及亚细胞定位分析[J].北方园艺,2018,42(03):92-100.[doi:10.11937/bfyy.20172419]
　LI Zehong,YUAN Honghui,CHENG Hua,et al.Cloning and Subcellular Localization Analysis of GbERF1 Transcription Factor in Ginkgo biloba L.[J].Northern Horticulture,2018,42(03):92-100.[doi:10.11937/bfyy.20172419]
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银杏GbERF1转录因子基因的克隆及亚细胞定位分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年03 页码: 92-100 栏目: 出版日期: 2018-02-15
Title:
Cloning and Subcellular Localization Analysis of GbERF1 Transcription Factor in Ginkgo biloba L.
作者:
李泽宏1; 袁红慧2; 程华2; 李琳玲2; 冯如1; 程水源1
1.武汉轻工大学 生物与制药工程学院，湖北 武汉 430023；2.黄冈师范学院 生命科学学院，湖北 黄冈 438400
Author(s):
LI Zehong1; YUAN Honghui2; CHENG Hua2; LI Linling2; FENG Ru1; CHENG Shuiyuan1
1.School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan,Hubei 430023；2.College of Life Sciences,Huanggang Normal University,Huanggang,Hubei 438400
关键词:
银杏; ERF转录因子; 荧光定量分析; 亚细胞定位
Keywords:
Ginkgo biloba L.; ERF transcription factors; fluorescence quantitative analysis; subcellular localization
DOI:
10.11937/bfyy.20172419
文献标志码:
A
摘要:
ERF是植物中一类重要的转录因子，广泛参与植物对生物和非生物胁迫的响应。以3年生银杏苗为试材，采用RT-PCR与RACE-PCR方法，研究了银杏ERF基因序列，以期深入了解银杏药用成分合成代谢的分子机理。结果表明：GbERF1含有长度为1 314 bp的完整开放阅读框，编码437个氨基酸。蛋白序列分析显示，GbERF1蛋白含有2段ERF家族的特殊位点。GbERF1蛋白序列与北美云杉（ADE75663.1）序列的相似性为97%。系统进化树分析，GbERF1与北美云杉、狭叶羽扇豆的序列可划归为同一分支。实时荧光定量PCR结果显示，GbERF1主要在银杏根和叶中表达，逆境胁迫处理后显示，该基因在乙烯、紫外诱导处理下，表达量大幅度提升，随后下降，但仍高于对照组，干旱诱导处理下表达量先升高后降低，矮壮素(CCC)诱导下表达量变化不太明显。烟草叶片下表皮细胞亚细胞定位结果表明GbERF1定位于细胞核。
Abstract:
ERF transcription factors are important in plants,which are widely involved in plant response to biotic and abiotic stress.Three-year-old Ginkgo biloba L. tree was used as materials,the sequences of ERF was explored by using RT-PCR and RACE-PCR technology,in order to understand the molecular mechanism of medicinal component metabolism of Ginkgo biloba L.The results showed that GbERF1 contained a 1 314 bp ORF encoding 437 amino acid residues.NCBI-BLAST showed GbERF1 protein had two specific hits of ERF superfamily.GbERF1shared 97% similarity with sequences of Picea sitchensis.Phylogenetic tree analysis showed that GbERF1 was grouped into the same clade with sequences of Picea sitchensis and Lupinus angustifolius.The expression patterns of GbERF1 genes in Ginkgo biloba L.tissues and under stress treatments were analyzed by real-time quantitative PCR,the results showed that GbERF1 genes expressed highly in root and leaf.The expression level of GbERF1 under the treatment of ET and UV was significant higher than that of control group,and expression trend of them was upgrade at first and then descend.The expression trend of them under drought was upgrade at first and then descend,and the expression difference was not significant under CCC treatment.Following transient expression of GbERF1 in epidermal cells of leaf of tobacco,GbERF1 was found to be localized in the nucleus.

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备注/Memo

第一作者简介：李泽宏（1992-），男，硕士研究生，研究方向为药物化学。E-mail：lee_zeno@foxmail.com.

责任作者：程水源（1965-），男，教授，博士生导师，研究方向为经济林种质资源评价与利用。E-mail：s_y_cheng@sina.com.
基金项目：湖北省高端人才引领培养计划资助项目（鄂科技通[2012]86号）；国家自然科学基金资助项目（31270717，31400556）；湖北省自然科学重点资助项目（2013BHE029）。
收稿日期：2017-09-30

更新日期/Last Update: 2018-02-09