WANG Yuying,LI Lingli,LIU Dan,et al.Rapid Propagation of Cymbidium faberi Rolfe var.Szechuanicum×（C.tracyanum var.Huanghua×C.mastersii） F1 hybrids[J].Northern Horticulture,2018,42(03):87-91.[doi:10.11937/bfyy.20172250]
‘春绿兰×虎雪兰’F1代组培苗快繁技术
- Title:
- Rapid Propagation of Cymbidium faberi Rolfe var.Szechuanicum×（C.tracyanum var.Huanghua×C.mastersii） F1 hybrids
- 文献标志码:
- A
- 摘要:
- 以‘春绿兰×虎雪兰’F1代的原球茎为外植体，在无菌条件下，以1/2MS培养基为基本培养基，添加不同浓度6-BA与NAA，研究了不同激素配比对原球茎不同培养阶段的增殖、分化和生根的影响。结果表明：1/2MS+1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA+80 g?L-1香蕉泥，利于原球茎的增殖和分化，增殖率和分化率分别为222.00%、90.57%；1/2MS＋1.5 mg?L-1 6-BA＋0.5 mg?L-1 NAA+80 g?L-1香蕉泥，利于不定芽增殖，增殖率为42.50%；1/2MS＋0.5 mg?L-1 6-BA＋1.5 mg?L-1 NAA生根效果最佳，生根率为100%，平均株根数为5.50条，平均根长为40.73 mm，且植株长势健壮。
- Abstract:
- The Cymbidium faberi Rolfe var.Szechuanicum×（C.tracyanum var.Huanghua×C.mastersii） F1 hybrids as test material,protocorm-like-body （PLB) were used as explants in tissue culture to study the influence of BA and NAA in different concentrations,mashed bananas and activated carbon on their regeneration under 1/2MS basic medium.The results showed that 1/2MS+1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA+80 g?L-1mashed bananas could make PLB rate to 222.00% and differentiation rate to 90.57%,1/2MS＋1.5 mg?L-1 6-BA＋0.5 mg?L-1 NAA+80 g?L-1 mashed bananas could make multiplication rate to 42.50%,1/2MS＋0.5 mg?L-1 6-BA＋1.5 mg?L-1 NAA could make rooting rate to 100%,the average number of plant roots was 5.50,average root length was 40.73 mm,and plant grew healthily.
参考文献/References:
[1]任建青，李枝林.虎雪兰栽培的施肥试验[J].农业科技通讯，2013（12):129-132.
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备注/Memo
第一作者简介：王玉英（1980-),女，云南大理人，博士，副教授，现主要从事植物资源的利用和创新等研究工作。E-mail:wyysxp@126.com.