[1]龙良鲲,梁子豪,林群英,等.一株蝉花的ITS序列分析及产纤溶酶条件的研究[J].北方园艺,2018,42(03):28-33.[doi:10.11937/bfyy.20171627]
　LONG Liangkun,LIANG Zihao,LIN Qunying,et al.Identification of a Cordyceps cicadae Strain Based on ITS Sequences and Production of Fibrinolytic Enzymes by the Fungus[J].Northern Horticulture,2018,42(03):28-33.[doi:10.11937/bfyy.20171627]
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一株蝉花的ITS序列分析及产纤溶酶条件的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年03 页码: 28-33 栏目: 出版日期: 2018-02-15
Title:
Identification of a Cordyceps cicadae Strain Based on ITS Sequences and Production of Fibrinolytic Enzymes by the Fungus
作者:
龙良鲲1; 梁子豪1; 林群英2; 胡毅1; 吴文杰1; 丁少军1
1.南京林业大学 化学工程学院，江苏 南京 210037；2.中华全国供销合作总社 南京野生植物综合利用研究院，江苏 南京 210042
Author(s):
LONG Liangkun1; LIANG Zihao1; LIN Qunying2; HU Yi1; WU Wenjie1; DING Shaojun1
1.College of Chemical Engineering,Nanjing Forestry University,Nanjing,Jiangsu 210037;2.Nanjing Institute for the Comprehensive Utilization of Wild Plants,All China Federation of Supply and Marketing Cooperatives,Nanjing,Jiangsu 210042
关键词:
蝉花; 纤溶酶; 营养条件; 液体发酵
Keywords:
Cordyceps cicadae; fibrinolytic enzyme; nutritional sources; liquid culture
DOI:
10.11937/bfyy.20171627
文献标志码:
A
摘要:
以新分离的蝉花（Cordyceps cicadae）CC1504为试材，通过真菌核糖体DNA内转录间隔区（ITS)序列分析确定菌株的分类学地位；利用琼脂糖-纤维蛋白平板法测定蝉花产纤溶酶的能力，通过单因素试验确定其液体发酵产纤溶酶的最适碳源和最适氮源；以正交实验优化菌株产纤溶酶的培养基组成。结果表明：ITS序列进化树分析确定了该菌株的分类地位为蝉花；该蝉花产纤溶酶的最适碳源为乳糖，产酶水平为248.95 U?mL-1，其次是甘油，为198.94 U?mL-1；蝉花产纤溶酶的最适氮源为蛋白胨，产酶水平可达240.68 U?mL-1，其次是酵母膏，为89.23 U?mL-1；获得的最佳培养基碳氮源组成为：乳糖10 g?L-1、蛋白胨10 g?L-1、酵母膏3 g?L-1，可发酵获得的纤溶酶水平达262.05 U?mL-1。
Abstract:
A newly isolated Cordyceps cicadae strain CC1504 was used as research material in this study.The taxonomy position of the fungus was confirmed by analysis of its internal transcribed spacer （ITS) sequences of ribosomal DNA.Agarose-fibrin plate method was employed to detect the fibrinolytic enzymes activity produced by the fungal strain.The carbon or nitrogen sources for the production of fibrinolytic enzymes were screened by single-factor experiments.Orthogonal experimental design was conducted to optimize the medium component for fibrinolytic enzymes production.The results indicated that the fungal strain CC1504 belonged to Cordyceps cicadae according to the phylogenetic tree analysis based on ITS sequences.The optimal carbon source was lactose for the production of fibrinolytic enzymes,and the highest production level up to 248.95 U?mL-1,followed by glycerol,given the yield at 198.94 U?mL-1.Peptone was the optimal nitrogen source for the production of enzymes,giving maximum level at 240.68 U?mL-1,and followed by yeast extract with production level of 89.23 U?mL-1.The optimized medium formula （carbon and nitrogen sources) was as:lactose 10 g?L-1,peptone 10 g?L-1 and yeast extract 3 g?L-1,giving the production of 262.05 U?mL-1.

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相似文献/References:

[1]林群英,封义,姜艳,等.蛹虫草液体培养产纤溶酶条件优化研究[J].北方园艺,2015,39(24):143.[doi:10.11937/bfyy.201524039]
　LIN Qunying,FENG Yi,JIANG Yan,et al.Optimization of Submerged Culture Conditions for Production of Fibrinolytic Enzyme by Cordyceps militaris[J].Northern Horticulture,2015,39(03):143.[doi:10.11937/bfyy.201524039]

备注/Memo

第一作者简介：龙良鲲（1978-），男，博士，副教授，现主要从事微生物活性物质等研究工作。E-mail:longlk602@njfu.edu.cn.

责任作者：丁少军（1964-），男，博士，教授，博士生导师，现主要从事微生物酶与生物技术等研究工作。E-mail:dshaojun@hotmail.com.
基金项目：江苏省高校自然科学基金资助项目（15KJB220003）；南京林业大学大学生创新训练计划资助项目（2015sjcx033）；江苏省大学生科技创新计划资助项目（201610298104X）。
收稿日期：2017-07-18

更新日期/Last Update: 2018-02-09