CHEN Gang,WANG Yinghua,JIN Hong.Tissue Culture and Plant Regeneration of Melastoma candidum var.albiflorum[J].Northern Horticulture,2017,41(11):119-124.[doi:10.11937/bfyy.201711024]
白花野牡丹的组织培养及植株再生
- Title:
- Tissue Culture and Plant Regeneration of Melastoma candidum var.albiflorum
- 文献标志码:
- A
- 摘要:
- 以白花野牡丹带节茎段为外植体，研究了基本培养基种类及植物生长调节剂对丛生芽诱导的影响，并进行了生根培养基的筛选。结果表明：白花野牡丹的芽分化优化培养基为1/4MS+1.0 mg?L-1 6-BA；优化的生根培养基为1/2MS+0.1 mg?L-1 IBA；经练苗后移栽成活率在90%以上，1年后成活植株可以开花结实。
- Abstract:
- Stems of Melastoma candidum var.albiflorum was used as explants.The effects of types of basic medium and plant growth regulators on multiple shoots inducing were studied，and the optimum medium for rooting was selected.The results showed that 1/4MS basic medium containing 1.0 mg?L-1 6-BA was the most effective in inducing multiple shoots.The optimal culture medium for rooting was 1/2MS with 0.1 mg?L-1 IBA.The survival rate of the plantlets was up to 90% after being transplanted into soil，and one year later the plants could flower and seed normally.
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备注/Memo
第一作者简介：陈刚(1974-)，男，博士，副教授，现主要从事植物细胞工程和基因工程等研究工作。E-mail:chengang@zqu.edu.cn.责任作者：金红（1974-），女，博士，高级工程师，现主要从事植物生物多样性保护与细胞工程等研究工作。E-mail:Jinhong@szum.gov.cn.基金项目：深圳市城市管理局科研资助项目(201318)。