[1]杨文婷,黄士栩,翁德会,等.蕲艾挥发油体外抑菌作用及其机理[J].北方园艺,2017,41(23):22-28.[doi:10.11937/bfyy.20171040]
　YANG Wenting,HUANG Shixu,WENG Dehui,et al.Antibacterial Effect and Mechanism of Essential Oils From Artemisia argyi in vitro[J].Northern Horticulture,2017,41(23):22-28.[doi:10.11937/bfyy.20171040]
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蕲艾挥发油体外抑菌作用及其机理

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年23 页码: 22-28 栏目: 出版日期: 2017-12-10
Title:
Antibacterial Effect and Mechanism of Essential Oils From Artemisia argyi in vitro
作者:
杨文婷1; 黄士栩1; 翁德会2; 吴士筠1
1.武汉工商学院 环境与生物工程学院，湖北 武汉 430065；2.武汉华夏理工学院 生物与制药学院，湖北 武汉430223
Author(s):
YANG Wenting1; HUANG Shixu1; WENG Dehui2; WU Shijun1
1.Environmental and Biological Engineering College，Wuhan Technology and Bussiness University,Wuhan,Hubei 430065;2.College of Biology and Pharmaceutical, Wuhan Huaxia University of Technology, Wuhan，Hubei 430223
关键词:
蕲艾; 挥发油; 抑菌活性; 抑菌机理
Keywords:
Artemisia argyi; volatile oil; antibacterial activity; antibacterial mechanism
DOI:
10.11937/bfyy.20171040
文献标志码:
A
摘要:
以湖北蕲春地区道地药材蕲艾为试材，通过水蒸气蒸馏法提取了其挥发油成分，利用抑菌圈法和常量稀释法考察了蕲艾挥发油的抑菌效果，并对其抑菌机理进行了初步研究，以期为蕲艾的药用资源化研究提供参考依据。结果表明：蕲艾挥发油对不同微生物的抑菌效果为金黄色葡萄球菌＞大肠杆菌＞黑曲霉＞青霉，金黄色葡萄球菌抑菌圈直径达1.5 cm，对金黄色葡萄球菌的最小抑菌浓度为8.0 mg?mL-1。不同时间菌体内外蛋白质和核酸含量的变化结果表明，蕲艾挥发油可增加细菌细胞膜的通透性，并对细菌核酸合成有一定抑制作用。
Abstract:
Artemisia argyi was used as test material,the antibacterial activity of essential oils against different microorganism was studied by inhibition zone method and constant dilution method.The results showed that the antibacterial activity of essential oils were in the descending order of degree of influence were Staphylococcus aureus,Escherichia coli,Aspergillus niger and Penicillium.The inhibition zone diameter against Staphylococcus aureus reached 1.5 cm,and the minimal inhibitory concentration was 8.0 mg?mL-1.According to the content change of protein and nucleic acid,the antibacterial mechanism of essential oils was enhancement of cell membrane permeability and inhibition of synthesis of nucleic acid.

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备注/Memo

第一作者简介：杨文婷（1983-），女，硕士，副教授，现主要从事天然产物的开发利用等研究工作。E-mail：47389578@qq.com.

责任作者：吴士筠（1950-），女，本科，高级实验师，研究方向为天然产物的开发利用。E-mail：779642941@qq.com.
基金项目：湖北省教育厅科学技术研究资助项目（B2017394）；武汉工商学院校级学术团队基金资助项目（XSTD2015003）。
收稿日期：2017-07-18

更新日期/Last Update: 2017-12-15