ZHANG Hong,ZHENG Rong.Study on Diversity and Community Characteristics of Soil Fungi at Burned Forest Sites for the Great Xing′an[J].Northern Horticulture,2017,41(10):159-166.[doi:10.11937/bfyy.201710037]
大兴安岭火烧迹地土壤真菌群落结构及多样性研究
- Title:
- Study on Diversity and Community Characteristics of Soil Fungi at Burned Forest Sites for the Great Xing′an
- 文献标志码:
- A
- 摘要:
- 以大兴安岭火烧迹地在不同火烧年和不同火烧程度的土样为研究对象，揭示大兴安岭火烧迹地恢复过程中土壤真菌的多样性与群落变化特征，为火烧后森林生态系统的科学管理和森林更新提供理论依据。用PCR-DGGE法对土壤真菌进行了分析，采用Quantity one软件分析其多样性，通过序列的同源性比较构建系统发育树，真菌结构主成分分析采用Canoco等软件。结果表明：每个样品真菌种类丰富，不同样品有相同的真菌菌属也有不同的真菌菌属，17个优势条带测序所得菌属与黏滑菇属（Hebeloma）、蜡伞属（Hygrophorus）、蜡壳菌属（Sebacima）、管柄囊霉属（Funneliformis）亲缘关系最近，其中还有一些不可培养的真菌。不同火烧年和火烧程度之间土样真菌香农指数、丰富度、均匀度均达到显著差异（P<0.05）。不同火烧程度、不同火烧年均能影响土壤真菌的群落结构。
- Abstract:
- There were soil samples of different burned year and different burned degree to study at burned forest site for Great Xing′an,which would reveal diversity of soil fungi and variational characteristic of community,and provide theoretical basic for the science management of forest ecosystem and forest regeneration.The soil fungal characteristics were analyzed by PCR-DGGE method.Quantity one was used to analyze the diversity，and compared sequence homology to construct phylogenetic tree.Principal component analysis of fungi were analyzed using Canoco software.The results showed that each fungus was rich in species，of which had the same fungus species and different fungus species.and the 17 preponderant bands had a close relationship with Hebeloma，Hugrophorus，Sebacima，Funneliformis，and some uncultured fungi.There was a significant influence on different soil fungal Shannon index(H)，richness(S)，evenness(EH) in the different year and different burned degree(P<0.05).And different burned degree and different burned year also influenced soil fungal structure.
参考文献/References:
[1]张艳平.黑龙江大兴安岭地区气候变化对森林火灾影响的研究[D].哈尔滨:东北林业大学,2008. [2]王丽红,辛颖,赵雨森,等.大兴安岭火烧迹地在植被恢复中土壤微生物生物量和酶活性[J].水土保持学报,2015,29(3):185-189. [3]胡宇,蒋先军,田波,等.稻田垄作免耕对土壤真菌多样性分布的影响[J].土壤,2007,39(6):964-967. [4]郝桂玉,黄民生,徐亚同.蚯蚓及其在生态环境保护中的应用[J].环境科学研究,2004,17(3):75-77. [5]PAUL E A.Soil microbiology,ecology and biochemistry[M].New York:Academic Press,2014. [6]HEIDJEN M G A,BARDGETT R D,STRAALEN N M,et al.The unseen majority:soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems[J].Ecology Letters,2008,11(3):296-310. [7]孙龙燕,李士美,李伟,等.林火对植物根围丛枝菌根真菌多样性的影响[J].生态学报,2016,36(10):2833-2841. [8]阮晓东,张惠文,孙冬雪,等.油松阔叶混交林不同层次优势植被根区土壤真菌的群落结构[J].东北林业大学学报,2009,37(5):48-50. [9]CERTINI G.Effects of fire on properties of forest soils:a review[J].Oecologia,2005,143(1):1-10. [10]许鹏波,屈明,薛立.火对森林土壤的影响[J].生态学杂志,2013,32(6):1596-1606. [11]KARA O,BOLAT I.Short-term effects of wildfire on microbial biomass and abundance in black pine plantation oils in Turkey[J].Ecological Indicators,2009,9(6):1151-1155. [12]宝日玛,峥嵘,周梅,等.大兴安岭火烧迹地土壤微生物生物量及酶活性研究[J].内蒙古农业大学学报,2016,37(4):78-82. [13]张敏,周鹏,季永华.苦楝-小麦农林复合生态系统土壤真菌群落结构分析[J].林业科学,2015,51(10):26-34. [14]VALLAEYS T,TOPP E,MUYZER G,et al.Evaluation of denaturing gradient gel electrophoresis in the detection of 16S rDNA sequence variation in rhizobia and methanotrophs[J].FEMS Microbiology Ecology,1997,24(3):279-285. [15]MUYZER G,WAAL E C,UITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J].Applied and Environmental Microbiology,1993,59(3):695-700. [16]金海林.DGGE技术在人参重茬地土壤微生物多样性分析中的应用研究[D].延吉:延边大学,2010. [17]张敏,胡海清.林火对土壤微生物的影响[J].东北林业大学学报,2002,30(4):44-46. [18]尚玉昌.普通生态学[M].北京:北京大学出版社,2010. [19]芦雪妍,崔晓阳.大兴安岭森林火烧对土壤生境质量影响研究[J].中国农业资源与区划,2016,37(2):76-78. [20]MONLEON V J,CROMACK J K,LANDSBERG J D.Short-and long-term effects of prescribed underburning on nitrogen availability in ponderosa pine stands in central Oregon[J].Canadian Journal of Forest Research,1997,27(3):369-378. [21]周涧青,刘晓东,张思玉,等.不同火烧时间对杉木人工林土壤性质的影响[J].西北林学院学报,2016(3):36-40. [22]孙家宝,胡海清.大兴安岭兴安落叶松林火烧迹地群落演替状况[J].东北大学学报,2010,38(5):30-33. [23]CULTER D R,EDWARDS T C,BEARD K H,et al.Random forests for classification in ecology[J].Ecology,2007,88(11):2783-2792. [24]IAN C A,BRIGITTE A B,DAVID R G.Asidiomycete fungal communities in Australian sclerophy forest soil are altered by repeated prescribed burning[J].Mycological Research,2007,111(4):482-486. [25]BASTIAS B A,HUANG Z Q,BLUMFIELD T,et al.Influence of repeated prescribed burning on the soil fungal community in an eastern Australian wet sclerophyll forest[J].Soil Biology and Biochemistry,2006,38(12):3492-3501.
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备注/Memo
第一作者简介：张虹（1992-），女，硕士研究生，研究方向为菌根生物技术。E-mail:zhanghong09.27@qq.com.责任作者：峥嵘（1971-），女，博士，副教授，硕士生导师，现主要从事菌根生物技术等研究工作。E-mail:zhengrong09@163.com.基金项目：内蒙古自治区科技计划资助项目（20130435）。