SUN Xiangke,LI Yuanyuan,LIU Xue,et al.Effects of Different Hormones on Regeneration System of ‘Colorful pearl’ Tomato[J].Northern Horticulture,2017,41(08):85-89.[doi:10.11937/bfyy.201708020]
不同激素对“五彩珍珠”番茄再生体系的影响
- Title:
- Effects of Different Hormones on Regeneration System of ‘Colorful pearl’ Tomato
- Keywords:
- Solanum lycopersicum ‘Colorful pearl’; cotyledon; hypocotyledonary axis; regeneration system
- 文献标志码:
- A
- 摘要:
- 以“五彩珍珠”番茄为试材，选取6 d苗龄无菌苗的子叶和下胚轴为外植体，研究了不同激素浓度及配比对番茄愈伤组织的诱导、不定芽分化及生根的影响，以建立番茄再生体系。结果表明：诱导“五彩珍珠”番茄愈伤组织的最佳培养基为MS+0.4 mg?L-1IAA+2.0 mg?L-1 6-BA，其子叶的诱导率为100%，下胚轴的诱导率为95%；不定芽分化的最佳培养基为MS+0.2 mg?L-1 IAA+(1.0~3.0)mg?L-1 6-BA；诱导生根的最佳培养基为1/2MS+0.10 mg?L-1 NAA。子叶为最佳外植体，其不定芽的分化率及生长情况均优于下胚轴。
- Abstract:
- ‘Colorful pearl’ tomato was used as the test material,cotyledon and hypocotyledonary axis of 6 day-old sterile seedlings were used as explants to study the effect of different hormone concentration and hormone ratio on induction of tomato callus，differentiation of adventitious buds and rooting induction in order to establish regeneration system for tomato.The results showed that the optimum culture medium for induction of tomato callus was MS+0.4 mg?L-1IAA+2.0 mg?L-1 6-BA,in which the induction rate of cotyledon and hypocotyledonary axis was 100% and 95%,respectively.The optimum culture medium for differentiation of adventitious buds was MS+0.2 mg?L-1 IAA+(1.0-3.0)mg?L-1 6-BA;the optimum culture medium for rooting induction was 1/2MS+0.10 mg?L-1 NAA.It concluded that cotyledon was the optimal explant.Its adventitious bud differentiation and growth rates were better than those of hypocotyledonary axis.
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备注/Memo
第一作者简介：孙祥科（1992-），男，山东泰安人，硕士研究生，研究方向为生物材料的生物相容性。E-mail:578412902@qq.com.责任作者：宿烽(1965-),女，山东青岛人,博士,副教授，研究方向为生物材料的生物相容性。E-mail:sufengvip@126.com.基金项目：青岛市民生科技计划资助项目（16-6-2-17-nsh）；青岛科技大学“大学生创新创业训练计划”资助项目（201501004，201601007）。