ZHENG Xiqing,GUO Zhenhua,DI Na,et al.Isolation and Screening of Antagonistic Bacterium Against Botrytis cinerea[J].Northern Horticulture,2017,41(06):122-126.[doi:10.11937/bfyy.201706028]
番茄灰霉病拮抗细菌的分离筛选及鉴定
- Title:
- Isolation and Screening of Antagonistic Bacterium Against Botrytis cinerea
- Keywords:
- Botrytis cinerea; antagonistic bacteria; screening
- 文献标志码:
- A
- 摘要:
- 以番茄灰霉病菌（Botrytis cinerea）为供试菌，采用平板对峙方法，研究了不同植物根围土壤中的细菌对番茄灰霉病（Botrytis cinerea）的拮抗效果。结果表明：对分离纯化的75株细菌进行拮抗测定，其中30株对番茄灰霉病有不同程度抑制作用，分离频率为40.00%。其中3株拮抗细菌YM8、FQ10、FQ11对番茄灰霉病病原菌拮抗效果较好，抑菌圈直径在1.5~3.0 cm。通过生理生化试验、形态特征观察对其进行了种属初步鉴定，鉴定结果为菌株YM8为地衣芽孢杆菌（Bacillus licheniformis），FQ10为短芽孢杆菌(Bacillus brevis)，FQ11为蜡状芽孢杆菌(Bacillus cereus)。
- Abstract:
- Botrytis cinerea Pers.was used as test fungus.Plate confrontation method was carried out to obtain antagonistic bacteria against Botrytis cinerea Pers.from different soil around the plant roots.The results showed that 75 bacteria were isolated and purified from different ecological soil samples.After primary screening test of tablet confrontation method,30 bacteria which had certain function of antagonism against Botrytis cinerea were verified.Separation frequency was 40.00%.And the antagonist bacteria YM8,FQ10,FQ11 had better inhibiting effect.According to the comparison,it was found that the bacteriostatic diameters of antagonistic bacteria against Botrytis cinerea was 1.5-3.0 cm,such as YM8,FQ10,FQ11.After the morphological observation and a series of physiological and biochemistry experiments,the bacterial strain YM8 was primarily identified as Bacillus licheniformis,FQ10 belonged to Bacillus brevis,FQ11 belonged to Bacillus cereus.
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备注/Memo
第一作者简介：郑喜清（1979-），女，内蒙古兴安盟人，硕士，讲师，现主要从事植物病害综合防控技术等研究工作。E-mail:413703219@qq.com.基金项目：内蒙古自治区高等学校青年科技英才支持计划资助项目（NJYT-15-B23）；内蒙古河套学院自然科学资助项目（HTXYZY13005）。