[1]刘晓婷,孙国琴,李亚娇,等.一株白桩菇属野生食用菌菌种分离鉴定与培养[J].北方园艺,2017,41(18):161-166.[doi:10.11937/bfyy.20170461]
　LIU Xiaoting,SUN Guoqin,LI Yajiao,et al.Isolation,Identification and Training for a Wild Fungus of Genus Leucopaxillus[J].Northern Horticulture,2017,41(18):161-166.[doi:10.11937/bfyy.20170461]
点击复制

一株白桩菇属野生食用菌菌种分离鉴定与培养

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年18 页码: 161-166 栏目: 出版日期: 2017-09-30
Title:
Isolation,Identification and Training for a Wild Fungus of Genus Leucopaxillus
作者:
刘晓婷1; 孙国琴2; 李亚娇2; 王淑妍1; 郭九峰1
1.内蒙古大学 物理科学与技术学院,离子束生物工程自治区重点实验室，内蒙古 呼和浩特 010021；2.内蒙古农牧业科学院，内蒙古 呼和浩特 010031
Author(s):
LIU Xiaoting1; SUN Guoqin2; LI Yajiao2; WANG Shuyan1; GUO Jiufeng1
1.Key Laboratory of Ion Beam Biotechnology/College of Physical Science and Technology,Inner Mongolia University,Huhhot,Inner Mongolia 010021;2.Inner Mongolia Academy of Agriculture and Livestock Sciences,Hohhot,Inner Mongolia 010031
关键词:
野生真菌; 形态特征; ITS序列; 分子鉴定方法; 原基
Keywords:
wild fungi; morphological characteristics; ITS gene sequence; molecular identification methods; primordium
DOI:
10.11937/bfyy.20170461
文献标志码:
A
摘要:
以内蒙古锡林郭勒草原一株野生蘑菇为试材，采用形态特征和生态习性鉴定及分子鉴定的方法，对该蘑菇的菌丝体基因组DNA进行18S和ITS片段的扩增、测序，测序结果经Blast比对，从而对该蘑菇进行分离鉴定。结果表明：该野生真菌属于白桩菇属Leucopaxillus。另外，通过优化的MS平板培养基避光培养，在MS平板培养基上有原基形成，为日后对该菌种的驯化与栽培提供材料，为其开发与利用奠定基础。其ITS序列已上传GenBank，登录号为KY173356。
Abstract:
A wild mushroom from Inner Mongolia′s Xilingol grassland was identified by methods of morphological characteristics and ecological habits of identification and molecular identification.Genomic DNA was extracted from mycelium then used as template for the 18S and ITS gene PCR amplification and sequencing.The 18S and ITS gene sequences were submitted to NCBI and aligned by Blast.The results showed that the fungi belonged to the genus Leucopaxillus.In addition,it had been found experimentally that the mycelium could be formed primordium in MS plate culture medium in dark.The ITS sequence was uploaded into NCBI,and GenBank accession number was KY173356.

参考文献/References:

[1]FEENEY M J,DWYER J,HASLER-LEWIS C M,et al.Mushrooms and health summit proceedings[J].J Nutr,2014,144(7)：1128-1136.

[2]BENJAMIN D R.Mushrooms：Poisons and panaceas[M].New York:WH Freeman and Co,1995.[3]张金霞,陈强,黄晨阳,等.食用菌产业发展历史、现状与趋势[J].菌物学报,2015(4):524-540.
[4]孔雷,张良,胡文洪,等.中国食用菌产业现状及预测[J].食用菌学报,2016(2):104-109.
[5]段福文,闫尔葵.楚雄地区4种野生菌子实体生长过程调查报告[J].中国食用菌,2015(1):34-36.
[6]王建瑞,刘宇,图力古尔.山东省大型真菌物种濒危程度与优先保育评价[J].生态学报,2015(3):837-848.
[7]张压宝.马龙县食用菌产业科技创新的现状及对策研究[J].中国食用菌,2015(3):78-81.
[8]庄海宁,张劲松,冯涛,等.我国食用菌保健食品的发展现状与政策建议[J].食用菌学报,2015(3):85-91.
[9]修翠娟.野生口蘑菌种分离及人工驯化[J].微生物学杂志,2012(3):98-100.
[10]张浩,张焕仕,王猛,等.我国食用菌栽培技术研究进展[J].北方园艺,2014(5):175-179.
[11]李玉.野生食用菌菌种分离与鉴定[D].福州:福建农林大学,2008.
[12]许峰,刘宇,王守现,等.一株野生大型真菌的分离与鉴定[J].中国农学通报,2012(13):176-179.
[13]RAJARATNAM S,THIAGARAJAN T.Molecular characterization of wild mushroom[J].European Journal of Experimental Biology,2012,2(2)：369-373.
[14]DAS S K,MANDAL A,DATTA A K,et al.Nucleotide sequencing and identification of some wild mushrooms[J].Sci World J,2013(11):403191.
[15]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi[J].PNAS,2012,109(16)：6241-6246.
[16]AVIN F A,BHASSU S,TAN Y S,et al.Molecular divergence and species delimitation of the cultivated oyster mushrooms：Integration of IGS1 and ITS[J].Sci World J,2014:793414.
[17]刘晓婷,郭九峰,王淑妍,等.蒙古口蘑担孢子萌发及初生菌丝生物学特性研究[J].北方园艺,2016(16):136-141.
[18]刘晓婷,郭九峰,王淑妍,等.用rDNA-ITS方法鉴别内蒙古多种野生食用菌[J].食药用菌,2015(5):301-306.
[19]黄年来.中国大型真菌原色图鉴[M].北京:中国农业出版社,1998.
[20]戴玉成,周丽伟,杨祝良,等.中国食用菌名录[J].菌物学报,2010,29(1):1-21.
[21]大自然博物馆编委会.大自然博物馆?百科珍藏图鉴系列：蘑菇[M].北京:化学工业出版社,2014.
[22]卯晓岚.中国大型真菌[M]郑州:河南科学技术出版社,2000.
[23]燕勇,李卫平,高雯洁,等.rDNA-ITS序列分析在真菌鉴定中的应用[J].中国卫生检验杂志,2008(10):1958-1960.

相似文献/References:

[1]田英翠,曹受金,曹基武.六种榉树种子形态特征及萌发特性的研究[J].北方园艺,2014,38(11):71.
　TIAN Ying-cui,CAO Shou-jin,CAO Ji-wu.Study on Morphology and Germination Characteristics of Seeds of Six Species of Zelkova schneideriana [J].Northern Horticulture,2014,38(18):71.
[2]陈春桦,高建明,刘巧莲,等.芦笋四倍体诱导、倍性鉴定及生物学特性的研究[J].北方园艺,2014,38(11):88.
　CHEN Chun-hua,GAO Jian-ming,LIU Qiao-lian,et al.Study on Tetraploid Induction,Identification and Biological Characteristics of Asparagus[J].Northern Horticulture,2014,38(18):88.
[3]张国珍,曾富春,李策宏,等.不同光照处理对五种观赏蕨生长发育及观赏特性的影响[J].北方园艺,2014,38(09):93.
　ZHANG Guo-zhen,ZENG Fu-chun,LI Ce-hong,et al.Effect of Different Light Treatment on Growth and Ornamental Feature of Five Ornamental Ferns[J].Northern Horticulture,2014,38(18):93.
[4]乌凤章.不同类型蓝莓种子形态及萌发特性研究[J].北方园艺,2013,37(21):32.
　WU Feng-zhang.Study on Seed Morphology and Germination Characteristics of Different Kinds of Blueberry[J].Northern Horticulture,2013,37(18):32.
[5]周彦珍,张志转,朱永和.苹果蠹蛾的识别与防治[J].北方园艺,2013,37(21):143.
　ZHOU Yan-zhen,ZHANG Zhi-zhuan,ZHU Yong-he.Identification and Control of Laspeyresia pomonella (Linnaeus)[J].Northern Horticulture,2013,37(18):143.
[6]郭玉,邵建柱,何建华,等.染色体加倍对“珠美”海棠形态特征的影响[J].北方园艺,2012,36(16):101.
　GUO Yu,SHAO Jian-zhu,HE Jian-hua,et al.Effect of Chromosome Doubling on Morphological Characteristics of Malus zumi [J].Northern Horticulture,2012,36(18):101.
[7]张文娟,李连国,郭金丽,等.干热胁迫下六种景天植物的形态特征及生理响应[J].北方园艺,2012,36(17):71.
　ZHANG Wen-juan,LI Lian-guo,GUO Jin-li,et al.Study on Morphological Characteristics and Physiological Response Under Drought and Heat Stress in Six Sedum Plants[J].Northern Horticulture,2012,36(18):71.
[8]杜社妮,白岗栓,边利军,等.河套灌区向日葵螟的识别与防治[J].北方园艺,2012,36(18):154.
　DU She-ni,BAI Gang-shuan,BIAN Li-jun,et al.Identification and Control of European Sunflower Moth in Hetao Irrigation District[J].Northern Horticulture,2012,36(18):154.
[9]孙红艳,张炳坤,周黎,等.蔡氏胡杨个木虱生物学特性及综合防治[J].北方园艺,2012,36(21):112.
　SUN Hong-yan,ZHANG Bing-kun,ZHOU Li,et al.Biological Characters and Integrated Control of Egeirotrioza ceardi Bergevin[J].Northern Horticulture,2012,36(18):112.
[10]赵娜娜,陈文强,邓百万,等.秦巴山区香菇主要栽培种亲缘关系的研究[J].北方园艺,2014,38(16):140.
　ZHAO Na-na,CHEN Wen-qiang,DENG Bai-wan,et al.Study of the Genetic Relationships of Main Lentinula edodes Cultispecies in Qinba Mountainous Area[J].Northern Horticulture,2014,38(18):140.

备注/Memo

第一作者简介：刘晓婷（1991），女，内蒙古乌海人，硕士研究生，研究方向为环境生物物理学。E-mail：liuxiaoting0522@sina.com.

基金项目：国家自然科学基金资助项目（51267014）。
收稿日期：2017-04-11

更新日期/Last Update: 2017-10-07