HAO Yaqiao,YAN Ling,WEI Xuan,et al.Effect of Cordyceps militaris′ Abandoned Cultivation Material on Quality of Pleurotus ostreatus[J].Northern Horticulture,2017,41(04):128-131.[doi:10.11937/bfyy.201704030]
人工北虫草废弃米基栽培辅料对平菇品质的影响
- Title:
- Effect of Cordyceps militaris′ Abandoned Cultivation Material on Quality of Pleurotus ostreatus
- Keywords:
- Cordyceps militaris; waste mediums; Pleurotus ostreatus; quality of Pleurotus ostreatus
- 文献标志码:
- A
- 摘要:
- 以北虫草废弃米基为试材，以平菇为指示食用菌，采取平菇栽培生长过程追踪、高效液相分析等方法，研究了废弃米基替代麦麸辅料对平菇菌丝生长、产量、菇体多糖和虫草素、腺苷含量的影响，以期在不降低平菇产量的条件下改善平菇品质及为虫草废弃米基提供新的利用途径。结果表明：北虫草废弃米基为部分或完全辅料栽培平菇，以完全或部分替代麦麸时，对供试平菇生物转化率无明显影响，但明显提高平菇菌丝生长速度，米基添加量为10%和20%时，菌丝生长速度分别提高了1.30倍和1.36倍；平菇子实体中多糖含量也显著提高，平菇采摘后菌糠中多糖含量也有所增加；平菇浸提液HPLC检测结果显示，平菇中各组腺苷含量无明显差别，但可以检测到虫草素。
- Abstract:
- In this study，the rate of mycelial growth，yield，fruitbody polysaccharide,adenosine and cordycepin of P.ostreatus were investigated when the waste medium of C.militaris was used as supplemental material.The fruitting test and HPLC method were used under the condition of not reducing the mushroom production in order to improve the quality of P.ostreatus and provide a new way to C.militaris waste mediums cyclic utilization.The results showed that C.militaris waste materials completely or partly replace the wheat bran had no significant effect on biomass transformation rate，but could improve the mycelium growth rate of P.ostreatus.When the abandoned cultivation material was added up to 10% and 20% as supplemental material，the growth rate of promycelium increased 1.30 times and 1.36 times respectively；the content of polysaccharide in P.ostreatus was significantly increased，as well as the content of polysaccharide in media was also increased；the cordycepin was detected in P.ostreatus by HPLC.
参考文献/References:
[1]范文丽,李天来,代洋,等.杏鲍菇、香菇、金针菇、蛹虫草、滑菇、平菇菌糠营养分析评价[J].沈阳农业大学学报,2013,44(5):673-677. [2]付彦凯,冷云伟,曹文化.蛹虫草的人工培育历程[J].北方园艺,2012(4):178. [3]李祥玲,胡劲松,陈作红.HPLC测定人工蛹虫草及其培养基中虫草素和腺苷含量[J].湖南师范大学（自然科学学报）,2010,33(2):107-111. [4]钟艳梅,黄志全,温凯.人工蛹虫草固体培养残基中虫草素的提取分离研究[J].现代食品科技,2007,23(2):40-42. [5]张颖,曾艳,张丽娇,等.蛹虫草菌糠多糖的分离纯化及结构组成分析[J].食品科学,2014,35(13):54-58. [6]胡珊,罗华建,黄皓,等.蛹虫草培养基残余体综合利用的研究进展[J].食用菌,2014(2):1-3. [7]孙诗清.蛹虫草培养基的综合利用研究[D].西安:西北大学,2005. [8]张丽艳.从蛹虫草栽培残渣中提取分离虫草素及其抑制肿瘤活性的研究[D].福州:福建师范大学,2010. [9]郭培红,宋学宏,孙丽萍,等.虫草培养基残余体在中华绒螯蟹饲料中的适宜添加量[J].淡水渔业,2011(3):43-49. [10]林群英,张锋伦,吴亮亮,等.蛹虫草及金针菇菌糠对杏鲍菇菌丝生长的影响[J].中国野生植物资源,2016(1):16-18. [11]刘红锦,蒋宁,李建军,等.蛹虫草多糖提取及纯化工艺研究[J].江西农业学报,2007,19(12):80-82. [12]孙兰凤,孙倩,潘琦,等.北虫草废料酶解液中核苷和虫草素HPLC分析[J].辽宁中医药大学学报,2014(6):49-50.
备注/Memo
第一作者简介：郝雅荞（1993-），女，辽宁朝阳人，硕士研究生，研究方向为生物化工。E-mail：hyq_synu@sina.com.责任作者：王升厚（1963-），男，辽宁沈阳人，本科，教授，硕士生导师，研究方向为微生物制药。E-mail：wshhwq2009@163.com.基金项目：辽宁科学事业公益基金资助项目（2014003020）。