[1]张剑,付亚娟.承德两种野生食用菌的分子鉴定及系统发育分析[J].北方园艺,2017,41(17):172-177.[doi:10.11937/bfyy.20170330]
　ZHANG Jian,FU Yajuan.Molecular Identification and Phylogenetic Analysis of Two Wild Edible Mushrooms in Chengde Area[J].Northern Horticulture,2017,41(17):172-177.[doi:10.11937/bfyy.20170330]
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承德两种野生食用菌的分子鉴定及系统发育分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年17 页码: 172-177 栏目: 出版日期: 2017-09-15
Title:
Molecular Identification and Phylogenetic Analysis of Two Wild Edible Mushrooms in Chengde Area
作者:
张剑1; 付亚娟2
1.廊坊职业技术学院，河北 廊坊 065000；2.廊坊师范学院 生命科学学院，河北省高校食药用菌应用技术研发中心，廊坊市食用菌技术重点实验室，河北 廊坊 065000
Author(s):
ZHANG Jian1; FU Yajuan2
1.Langfang Polytechnic Institute,Langfang,Heibei 065000；2.College of Life Science,Langfang Normal University/Edible and Medicinal Fungi Research and Development Center of Hebei Universities/Langfang Key Laboratory of Edible Fungi Technology,Langfang,Heibei 065000
关键词:
红蘑; 白蘑; ITS; 分子鉴定; 系统发育
Keywords:
red mushroom; white mushroom; ITS; molecular identification; phylogenetic analysis
DOI:
10.11937/bfyy.20170330
文献标志码:
A
摘要:
以承德红蘑和白蘑子实体为试材，采用真菌通用引物ITS1和ITS4，对其基因组rDNA基因的ITS区段进行PCR扩增、克隆测序，而后与GenBank数据库信息比对，并利用MEGA 5.0软件构建系统发育树，从分子水平上鉴定其种属地位及系统进化关系。结果表明：承德红蘑与血红铆钉菇（Chroogomphus rutilus，Accession No.：KR082876）、白蘑与Hygrophorus queletii（Accession No.：JF908069）的相似性均达到99%。承德红蘑与血红铆钉菇C.rutilus（Accession No.：KR082876），承德白蘑与H.queletii（Accession No.：JF908069）的遗传关系最近，遗传距离为0.002。因此，承德红蘑和白蘑经分子鉴定为C.rutilus和 H.queletii，其分类地位的解析，对今后野生红蘑和白蘑种质资源鉴定、生物学特性研究及开发利用奠定了基础。
Abstract:
In order to elucidate taxonomic status and phylogenetic relationship of wild red mushroom and white mushroom in Chengde area,the ITS regions of rDNA genes were amplified using ITS1 and ITS4 as primers,cloned and sequenced；ITS sequences were then analyzed via NCBI blast program,meanwhile phylogenetic tree were constructed using MEGA 5.0 software,respectively.The results indicated that the similarity of between red mushroom from Chengde area and Chroogomphus rutilus（Accession No.：KR082876）was up to 99%,and genetic distance was 0.002.White mushroom, which was another kind of precious wild edible fungi in Chengde area,also had 99% similarity with Hygrophorus queletii (Accession No.：JF908069),and their genetic distance reached to 0.002.Therefore,red mushroom and white mushroom in Chengde area were at the molecular level identified as C.rutilus and H.queletii,respectively.The elucidation of their taxonomic status would be beneficial for identification,biological characteristics,and development and utilization of wild red mushroom and white mushroom.

参考文献/References:

[1]张根伟，张丽萍，李书生．承德坝上松林野生食用菌资源[J]．中国农学通报，2012,28(28)：286-289.

[2]孙军德，丛日辉．血红铆钉菇不同部位组织分离的研血红铆钉菇SN菌株生物学特性研究[J]．菌物研究，2011,9(2)：105-109.
[3]董化研，郝册，牛玉蓉，等．血红铆钉菇生态学初步研究[J]．中国农学通报，2010,26(7)：191-194.
[4]WANG P,LIU Y,YIN Y G,et al.Diversity of microorganisms isolated from the soil sample surround Chroogomphus rutilus in the Beijing region[J].International Journal of Biological Sciences,2011,7(2)：209-220.
[5]田慧敏，文静，任芳．色钉菇（Chroogomphis rutillus）形态学和ITS测序鉴定[J]．江苏农业科学，2016,44(5)：261-263.
[6]冯建，秦淑亮，胡兵，等．色钉菇子实体化学成分及其生物活性初探[J]．菌物学报，2014,33(2)：355-364.
[7]LUO J,ZHANG C,ZHU H,et al.A new chromene from the fruiting bodies of Chroogomphus rutilus[J].Natural Product Research,2015,29(8)：698-702.
[8]THOMPSON J D,GIBSON T J,PLEWNIAK F,et al.The clustal_X windows interface：Flexible strategies for multiple sequence alignement aided by quality analysis tools[J].Nucleic Acid Research,1997(25)：4876-4882.
[9]TAMURA K,PETERSON D,PETERSON N,et al.MEGA5：Molecular evolutionary genetic analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011(28)：2731-2739.
[10]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi[J].Proceedings of the National Academy of Sciences of the United States of America,2012,109(16)：6241-6246.
[11]张微思，罗孝坤，张丽英，等．松茸菌丝体的纯培养及其鉴定[J]．中国食用菌，2010,29(3)：34-36.
[12]戴璐，李峻志，李安利，等．基于ITS序列分析对秦岭冷水沟羊肚菌的鉴定[J]．中国食用菌，2010,29(6)：45-46.
[13]王瑞，何艳华，管海华，等．对4个不同产地松乳菇子实体的分子鉴定[J]．江苏农业科学，2010(2)：37-39.
[14]刘晓婷，郭九峰，王淑妍，等．用 rDNA-ITS 方法鉴别内蒙古多种野生食用菌[J]．食药用菌，2015,23(5)：301-306.
[15]RO H S,KIM S S,RYU J S,et al.Comparative studies on the diversity of the edible mushroom Pleurotus eryngii：ITS sequence analysis,RAPD fingerprinting,and physiological characteristics[J].Mycological Research,2007,111：710-715.
[16]王波，郭勇，鲜灵．一种野生蘑菇的鉴定[J]．西南农业学报，2013,26(2)：672-675.
[17]黄龙花，杨小兵，张智，等．基于ITS序列分析鉴定灵芝属菌种[J]．中国食用菌，2010,29(1)：55-57.
[18]樊永军，赵艳玲，闫伟．贺兰山两株大型真菌物种分子鉴定与系统地位分析[J]．广东农业科学，2014(2)：155-156.

备注/Memo

第一作者简介：张剑（1981-），男，硕士，讲师，研究方向为微生物学。E-mail：zhangjian810916@163.com.

责任作者：付亚娟（1981-），女，博士，讲师，研究方向为分子微生物学。E-mail：fuyajuan501@163.com.
基金项目：2015年度河北省社会科学发展研究课题民生调研专项资助项目(201501519)；河北省高校食药用菌应用技术研发中心资助项目(YF201411-321)；廊坊师范人才引进科研启动资助项目(0140102001)；廊坊师范学院微生物学重点学科资助项目(2015001)。
收稿日期：2017-04-05

更新日期/Last Update: 2017-09-30