LI Yajie,LU Xiaomin.Effects of Putrescine on Growth，Antioxidant System and Photosynthesis of Cucumber Seedling Under Calcium Nitrate Stress[J].Northern Horticulture,2017,41(03):5-10.[doi:10.11937/bfyy.201703002]
腐胺对硝酸钙胁迫下黄瓜幼苗生长、抗氧化系统及光合作用的影响
- Title:
- Effects of Putrescine on Growth，Antioxidant System and Photosynthesis of Cucumber Seedling Under Calcium Nitrate Stress
- Keywords:
- cucumber; calcium nitrate stress; putrescine; antioxidant system; photosynthesis
- 文献标志码:
- A
- 摘要:
- 以“津春二号”黄瓜为试材，研究了硝酸钙胁迫下腐胺对黄瓜幼苗生长、抗氧化系统及光合作用的影响。结果表明：与对照相比，硝酸钙胁迫导致黄瓜幼苗叶片超氧阴离子产生速率、过氧化氢和丙二醛含量、膜透性显著升高，其净光合速率、气孔导度、蒸腾速率、胞间CO2浓度分别下降33.39%、55.18%、30.23%、34.56%，幼苗干物质积累减少44.17%，生长显著受抑；而腐胺可提高叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）等抗氧化酶活性，降低其超氧阴离子产生速率、过氧化氢和丙二醛（MDA）含量以及细胞膜透性，缓解硝酸钙胁迫下Pn值下降幅度，幼苗干物质积累增加21.59%，加快生长；可见，腐胺可通过调节硝酸钙胁迫下黄瓜幼苗抗氧化性以减少其膜脂过氧化程度，进而维持其较高的光合性能，有效促进了硝酸钙胁迫下黄瓜幼苗生长。
- Abstract:
- The effects of putrescine (Put) on growth，antioxidant system and photosynthesis of cucumber seedling ‘Jinchun No.2’ were studied under calcium nitrate stress.The results showed that，as compared with the control，calcium nitrate stress significantly increased the leaf H2O2 contents，lipid peroxidation and cell membrane permeability while decreased the leaf net photosynthetic rate(Pn)，stomatal conductance(Gs)，transpiration rate(Tr) and intercellular CO2 concentration(Ci) by 33.39%，55.18%，30.23%，34.56%，respectively，and dry matter accumulation reduced by 44.17%.Applying Put could increase the activities of SOD，POD and CAT，decrease the superoxide anion production rate，hydrogen peroxide contents，malondialdehyde contents and membrane permeability，alleviate the drop range of Pn，speed up the growth of seedlings and increase the dry matter accumulation by 21.59%.Therefore，Put treatment could keep a higher photosynthetic performance and effectively promote cucumber seedlings growth by adjusting the protective enzyme activity and reduce membrane lipid peroxide level under calcium nitrate stress.
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备注/Memo
第一作者简介：李雅洁（1992-），女，硕士研究生，研究方向为设施园艺及蔬菜生理生化。E-mail:liyajie302@163.com.责任作者：陆晓民（1969-），男，安徽固镇人,博士，教授，硕士生导师，现主要从事设施作物生理生态等研究工作。E-mail:luxiaomin88@163.com.基金项目：安徽省现代农业产业技术体系专项资助项目(AHCYTX-9)；安徽高校省级自然科学研究资助项目(KJ2013Z040)；安徽科技学院校级重点学科资助项目（AKZDXK2015C05）。