[1]贾慧梅,李康,康思璐,等.油点花的组织培养与快速繁殖[J].北方园艺,2017,41(21):104-108.[doi:10.11937/bfyy.20170245]
　JIA Huimei,LI Kang,KANG Silu,et al.Tissue Culture and Rapid Propagation of Ledebouria socialis (Liliaceae)[J].Northern Horticulture,2017,41(21):104-108.[doi:10.11937/bfyy.20170245]
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油点花的组织培养与快速繁殖

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年21 页码: 104-108 栏目: 出版日期: 2017-11-15
Title:
Tissue Culture and Rapid Propagation of Ledebouria socialis (Liliaceae)
作者:
贾慧梅; 李康; 康思璐; 蔡艳妮; 李会宁
陕西理工大学 生物科学与工程学院，陕西省资源生物重点实验室，陕西 汉中 723001
Author(s):
JIA Huimei; LI Kang; KANG Silu; CAI Yanni; LI Huining
College of Biological Science and Engineering,Shaanxi Sci-Tech University/Shaanxi Province Key Laboratory of Bio-resources,Hanzhong,Shaanxi 723001
关键词:
油点花; 外植体; 组织培养; 快速繁殖
Keywords:
Ledebouria socialis (Baker) Jessop; explants; tissue culture; rapid propagation
DOI:
10.11937/bfyy.20170245
文献标志码:
A
摘要:
以油点花Ledebouria socialis (Baker) Jessop的叶片、叶柄和假鳞茎为外植体，经表面消毒处理，接种于不同激素配比的MS培养基中，探讨油点花外植体诱导愈伤组织、丛生芽、不定根培养的最适培养基和培养条件，以获得大量种苗，为其人工种植及商业化水平发展提供参考依据。结果表明：不同外植体均能诱导愈伤组织的产生，且叶片外植体最易诱导；适合油点花愈伤组织诱导的最适培养基为MS+6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1，诱导率为96.3%～98.8%；而MS+6-BA 1.0 mg?L-1+NAA 0.1 mg?L-1适宜于芽诱导培养，诱导率为91.1%；培养基MS+NAA 0.2 mg?L-1适宜生根及壮苗培养，生根率89.5%；练苗移栽，其平均成活率达86.7%以上。
Abstract:
The leaf blade，petiole and bulbaceous from Ledebouria socialis (Baker) Jessop were used as explants,which were cultured on MS media supplemented with different hormones to establish a rapid propagation system.The results confirmed a reliable method,which could be used for mass propagation of L.socialis at the commercial level.The results showed that different explants could induce callus,while those from leaf blades were rather successful.The optimal medium for callus induction was MS+6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1,and the induction percentage was 96.3%-98.8%;MS+6-BA 1.0 mg?L-1+NAA 0.1 mg?L-1 were suitable for differentiation of adventitious buds,and with 91.1% in induction percentage.The medium MS+NAA 0.2 mg?L-1 was proper for forming roots,and rooting rate could be up to 89.5%.Transplanting experiment showed that the tissue cultured seedling grew with more than 86.7% survival rate.

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备注/Memo

第一作者简介：贾慧梅（1992-）,女，硕士研究生，研究方向为植物生物技术。E-mail：happygo21@126.com.

责任作者：李会宁（1962-），女，本科，高级实验师，研究方向为植物生物技术。E-mail：lhn@snut.edu.cn.
基金项目：陕西省教育厅基金资助项目（12JS026）;陕西省“13115”工程中心资助项目（2010ZDGC-23）。
收稿日期：2017-04-24

更新日期/Last Update: 2017-11-14