ZOU Yuan,WANG Xiaolong,CHEN Yaoyao,et al.Accumulation，Subcellular Distribution and Chemical Forms of Cadmium in Celery(Apium graveolens L.) Roots[J].Northern Horticulture,2017,41(02):31-37.[doi:10.11937/bfyy.201702006]
芹菜根对镉的累积及亚细胞和化学形态分布研究
- Title:
- Accumulation，Subcellular Distribution and Chemical Forms of Cadmium in Celery(Apium graveolens L.) Roots
- Keywords:
- cadmium (Cd); celery (Apium graveolens L.); accumulation; chemical form; subcellular distribution
- 文献标志码:
- A
- 摘要:
- 以芹菜品种“津南实芹1号”为试材，利用水培试验，对镉胁迫下芹菜生长、Cd吸收及亚细胞分布和化学赋存形态变化进行了研究。结果表明：芹菜植株地上部在Cd胁迫下并没有表现出明显的受毒害症状，但在高浓度Cd（40 mg?L-1）胁迫的环境下根系生长明显受到抑制。根系对Cd具有较强的积累能力，富集Cd的比例分配趋势表现为可溶性组分＞细胞壁＞细胞器，分析表明随着营养液中Cd浓度的增加，可溶性组分和细胞壁组分中Cd含量均显著增加，最高含量可达47.84 mg?kg-1 FW和113.60 mg?kg-1 FW。Cd浓度的变化对芹菜根组织中Cd化学形态分布的变化也有明显影响，当Cd浓度≤20 mg?L-1时，NaCl提取态Cd所占比例最高（32.45%~41.07%），说明与蛋白质、果胶酸类物质相结合或吸附的Cd比例较高；但当Cd浓度达到40 mg?L-1时，C2H5OH提取态Cd所占比例达到最高水平（62.30%），说明高Cd胁迫会造成细胞壁及质膜透性的增加，使Cd能以活性态存在于胞内；在所有Cd处理条件下HCl提取态Cd和HAc提取态Cd含量最低，说明芹菜根细胞壁中难溶性磷酸盐和草酸类物质含量较低，因而对Cd的固定化作用有限。
- Abstract:
- A hydroponics experiment combined with subcellular fractionation and sequential extraction was conducted to study the Cd concentration in different organs of celery and the Cd subcellular distribution and chemical forms in the roots with different Cd doses in nutrient solution.The results showed that no apparent injuries could be observed in the aerial part of celery.However，root growth was restrained greatly under high Cd treatment.The roots demonstrated high accumulation capacity of Cd，the Cd subcellular distribution was in the order of soluble fraction＞cell wall＞organelle.In addition，the Cd content in both the soluble fraction(47.84 mg?kg-1 FW to the at most) and the cell wall (113.60 mg?kg-1FW to the at most) showed increasing trend with elevated Cd supply.Chemical forms of Cd in root changed with the Cd concentration as well.When the Cd concentration in solution was less than or equal to 20 mg?L-1，the NaCl-extractable Cd was the highest (32.45%-41.07%)，which indicated that the combination of Cd with protein and pectic acid might play the dominant role；when Cd supply level was up to 40 mg?L-1，the C2H5OH-extractable Cd was the highest(62.30%)，which suggested that because of the high concentration of Cd stress，the cell wall and the membrane permeability increased，so the Cd ions were active in the cell，the HCl-extractable Cd and HAc-extractable Cd were the lowest under all Cd treatments，which indicated that the content of insoluble phosphate and oxalic acid in celery root cell wall was supposed to be relatively lower，contributing to the limited immobilization of Cd.
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备注/Memo
第一作者简介：邹圆（1995-），女，江西抚州人，本科，研究方向为土壤重金属植物污染化学及农产品安全。E-mail：zouyuan132@sina.com.责任作者：徐劼（1975-），男，博士，讲师，现主要从事土壤重金属植物污染化学及农产品安全等研究工作。E-mail：xujie1688@126.com.基金项目：嘉兴市科技计划资助项目（2012AY1046）；嘉兴学院重点“SRT计划”资助项目（851715059）。