WANG Yong,ZHOU Xuemei,SHENG Jie,et al.Pathogen Identification and Biological Characteristics of Onion Violet Leaf Spot[J].Northern Horticulture,2016,40(23):111-116.[doi:10.11937/bfyy.201623026]
洋葱紫斑病病原菌鉴定及生物学特性
- Title:
- Pathogen Identification and Biological Characteristics of Onion Violet Leaf Spot
- Keywords:
- onion violet leaf spot; Alternaria porri (Ell.) Cifferri; mycelial growth; sporulation characteristics
- 文献标志码:
- A
- 摘要:
- 以黑龙江省3个地区洋葱紫斑病植株为试材，采用组织分离法分离洋葱紫斑病病原菌，对获得菌株进行致病性测定、形态学鉴定以及16s rDNA ITS序列分析，并研究其生物学特性。结果表明：该致病菌为葱链格孢菌（Alternaria porri (Ell.) Cifferri），该菌株在以葡萄糖为碳源时产孢量最高，但菌丝生长量最低；在以氯化铵为氮源时菌丝生长量和产孢量都达到最高。32 ℃，pH 7，光照条件为12 h光照/12 h黑暗交替培养菌丝生长量最高；24 ℃，pH 8，连续黑暗条件下产孢量最高。
- Abstract:
- The plant of onion violet leaf spot was used as test material which was isolated from onion in three areas of Heilongjiang Province.The pathogen of onion violet leaf spot was isolated by tissue isolation，and the pathogen was identified by morphological and 16s rDNA ITS sequence analysis and the biological characteristics were conducted.The results showed that the pathogen of onion violet leaf spot was Alternaria porri (Ell.) Cifferri.When glucose was used as carbon source，the spore production was the highest，but the amount of mycelium growth was the lowest.When the ammonium chloride was the nitrogen source，the amount of mycelium growth and spore production reached the highest.The mycelium growth was the highest at 32 ℃，pH 7，and the condition was 12 h light 12 h dark alternate culture；the spore production was the highest at 24 ℃，pH 8 under continuous dark conditions.
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备注/Memo
第一作者简介：王勇（1971-），女，博士，教授，硕士生导师，研究方向为蔬菜遗传育种。E-mail：yongwang@neau.edu.cn. 责任作者：陈典（1953-），男，教授，硕士生导师，研究方向为蔬菜遗传育种。E-mail：lwang2002@163.com. 基金项目：黑龙江省基金资助项目（C2015017）；黑龙江省攻关资助项目（GA15B103-7）。