HOU Zhifang,MENG Lulu,XU Shiquan,et al.RAPD and SSR Analyses of Genetic Diversity of Ginseng Germplasm From Different Habitats[J].Northern Horticulture,2016,40(23):96-101.[doi:10.11937/bfyy.201623023]
不同产地人参种质资源RAPD和SSR分析
- Title:
- RAPD and SSR Analyses of Genetic Diversity of Ginseng Germplasm From Different Habitats
- Keywords:
- ginseng; RAPD; SSR; genetic diversity
- 文献标志码:
- A
- 摘要:
- 以15个产地人参种质资源为试材，采用RAPD和SSR分子标记技术对其遗传多样性进行分析。结果表明：2种分子标记均能揭示不同地区人参种质间的遗传多样性。共筛选出11条RAPD随机引物，平均每条引物可扩增出3~17条DNA片段，共扩增出104条清晰条带，多态性条带100条，多态性百分率为96.15%，揭示供试材料间遗传相似系数(GS)为0.505 3~0.989 5，平均值为0.760 4。筛选出5对SSR引物，平均每对引物可扩增出1~8条DNA片段，共扩增出38条清晰条带，多态性条带35条，多态性百分率为92.11%，揭示供试材料间遗传相似系数(GS)为0.400 0~0.960 0，平均值为0.742 1。RAPD和SSR标记的聚类分析在分类上稍有差异，但总体趋势一致，RAPD、SSR及RAPD＋SSR均将样品聚为三大类，均能揭示它们之间的亲缘关系，为人参种质资源的收集与利用奠定了基础。
- Abstract:
- Taking ginseng germplasms from 15 regions as materials，the genetic diversity of ginseng (Panax ginseng C.A Meyer) produced in 15 regions was analyzed by using RAPD and SSR.The results showed that two kinds of molecular markers could reveal the genetic diversity among different regions.In this paper，11 random RAPD primers were selected，each primer could amplify 3-17 DNA fragments，eleven RAPD primers had amplified 104 clear bands，100 polymorphic bands，and the percentage of polymorphism was 96.15%.RAPD revealed for the test between genetic similarity coefficient (GS) was 0.505 3-0.989 5，average of 0.760 4.Selected five pairs of SSR primers，per pair of primers could amplify DNA fragments from one to eight，a total of 38 clear amplified bands，35 polymorphic bands，percentage of polymorphism was 92.11%，the test revealed the genetic similarity coefficient (GS) was 0.400 0-0.960 0，average of 0.742 1.RAPD and SSR markers constructed cluster diagram was little difference in classification，but the overall trend was consistent.The samples were integrated into three categories by RAPD，SSR and RAPD+SSR，respectively.It could reveal the relationships among them，and laid the theoretical foundation for the introduction and cultivation and breeding of ginseng germplasm.
参考文献/References:
[1]郑友兰，张崇禧，李向高.吉林人参的质量评价指标与方法[J].人参研究，2001，13(2):12-14. [2]杨成君.人参cDNA文库构建,EST与相关基因表达分析及EST-SSR标记建立[D].哈尔滨：东北林业大学,2008. [3]WILLIAMS J G K，KUBELIK A R，LIVAK K J，et al.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Research，1990，18(22):6531-6535. [4]WELSH J，MCCLELLAND M.Fingerprinting genomes using PCR with arbitrary primers[J].Nucleic Acids Research，1990，18(24):7213-7218. [5]冯斗,吴子凯,陈荣基.RAPD方法在人参鉴别上应用研究初报[J].广西农业大学学报,1997,16(3):210-214. [6]任跃英，高巍，郭影,等.黄果及红果人参、西洋参基因组的RAPD分子标记研究[J].吉林农业大学学报，2005，27(1):39-42. [7]SCHLAG E M，MCINTOSH M S.The relationship between genetic and chemotypic diversity in American ginseng (Panax quinquefolius L.)[J].Phytochemistry，2013，93(1):96-104. [8]TAUTZ D.Hypervariability of simple sequences as a general source for polymorphic DNA markers[J].Nucleic Acids Res，1989,17(16)：6463-6471. [9]KIM N H，CHOI H I，AHN I O，et al.EST-SSR marker sets for practical authentication of all nine registered ginseng cultivars in Korea[J].Journal of Ginseng Research，2012，36(3):298-307. [10]CHOI H I，KIM N H，KIM J H，et al.Development of reproducible EST-derived SSR markers and assessment of genetic diversity in Panax ginseng cultivars and related species[J].Journal of Ginseng Research，2011，35(4):399-412. [11]REUNOVA G D，KOREN O G，MUZAROK T I，et al.Microsatellite analysis of Panax ginseng，natural populations in Russia[J].Chinese Medicine，2014，5(4):231-243. [12]喻景权,杜尧舜.蔬菜设施栽培可持续发展中的连作障碍问题[J].沈阳农业大学学报，2000，31(1):124-126. [13]汪立刚,沈阿林,孙克刚,等.大豆连作障碍及调控技术研究进展[J].土壤肥料，2001(5):3-8. [14]毛志泉,王丽琴,沈向,等.有机物料对平邑甜茶实生苗根系呼吸强度的影响[J].植物营养与肥料学报，2004，10(2):171-175. [15]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社，2000. [16]栾树昆，杨广顺，孙春玉,等.吉林抚松人参遗传多样性的SSR分析[J].北方园艺，2012(15):133-136. [17]杨广顺，王义，孙春玉,等.人参遗传多样性的SSR分析[J].安徽农业科学，2010，38(13):6660-6662.
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备注/Memo
第一作者简介：侯志芳（1991-），女，硕士研究生，研究方向为药用植物资源评价。E-mail：18243081801@163.com. 责任作者：郑培和（1973-），男，博士，副研究员，硕士生导师，现主要从事药用植物资源评价等研究工作。E-mail：zhengpeihe@caas.cn. 基金项目：国家国际科技合作专项资助项目（2015DFA31290）。