XI Yadong,CHEN Guohua,XIE Bingyan,et al.Control Effect of Different Trichoderma spp.Isolates on Phytophthora capsici[J].Northern Horticulture,2016,40(21):115-119.[doi:10.11937/bfyy.201621029]
不同木霉菌株对辣椒疫霉菌的防控作用
- Title:
- Control Effect of Different Trichoderma spp.Isolates on Phytophthora capsici
- Keywords:
- Trichoderma spp.; Phytophthora capsici; biocontrol; dimethomorph; increased production rate
- 文献标志码:
- A
- 摘要:
- 以6个不同种(Trichoderma hamatum、T.virens、T.citrinoviride、T.longibrachiatum、T.afroharzianum和T.asperelloides)的30株木霉菌株为试材,采用室内平板拮抗、温室盆栽和田间接种生防木霉菌的方法,研究了不同木霉菌对辣椒疫病的防治效果。结果表明:木霉菌对辣椒疫霉菌菌丝生长具有较好的抑制效果,不同木霉菌株间抑制率差异显著(P<0.05),平板拮抗抑制率在60.00%以上的菌株占总数的70%;木霉菌株T10(T.hamatum)的防控效果显著高于其它木霉菌株,在温室接种辣椒疫霉菌30 d后防控效果达到71.60%;在田间,木霉菌株T10对辣椒疫病的防治效果可达68.02%,与对照药剂烯酰吗啉的防治效果相当,且能够显著增加辣椒果实的产量,单株增产率达到14.30%,高于烯酰吗啉。
- Abstract:
- Taking 30 Trichoderma spp.isolates classified to 6 species (Trichoderma hamatum,T.virens,T.citrinoviride,T.longibrachiatum,T.afroharzianum and T.asperelloides) as materials,the biocontrol effect of different Trichoderma spp.isolates were studied by the antagonistic tests in vitro,inoculation tests under greenhouse and in field.The results showed that the Trichoderma spp.was an effective biological control effect for P.capsici.and the difference were significant (P<0.05) among different isolates.The Trichoderma spp.isolates number accounted for 70% for antagonistic efficacy above 60.00% in the mycelium of P.capsici in vitro antagonistic tests.Efficacy of T10 isolate against P.capsici reached 71.60% under greenhouse conditions when spraying spore suspension over P.capsici prior to inoculation of the pathogen.The biocontrol effect of isolates T10 against P.capsici was 68.02% and had not significant difference with dimethomorph at the 0.05 level in field.The results also showed that the T10 promoted dried chilli yield significantly,which was higher than dimethomorph treatment.
参考文献/References:
[1]LEONIAN L H.Stem and fruit blight of peppers caused by Phytophthora capsici sp.Nov[J].Phytopathology,1922(12):401-408. [2]BARKSDALE T H,PAPAVIZAS G C,JOHNSTON S A.Resistance to foliar blight and crown rot of pepper caused by Phytophthora capsici[J].Plant Disease,1984,68(6):506-508. [3]WEBER G F.Blight of peppers in Florida caused by Phytophthora capsici[J].Phytopathology,1932(22):775-780. [4]LAMOUR K H,MUDGE J,GOBENA D,et al.Genome sequencing and mapping reveal loss of heterozygosity as a mechanism for rapid adaptation in the vegetable pathogen Phytophthora capsici[J].Molecular Plant-Microbe Interactions,2012,25(10):1350-1360. [5]HARMAN G E.Overview of mechanisms and uses of Trichoderma spp.[J].Phytopathology,2006,96(2):190-194. [6]AHMED A S,PEREZ-SANCHEZ C,EGEA C,et al.Evaluation of Trichoderma harzianum for controlling root rot by Phytophthora capsici in pepper plants[J].Plant Pathology,1999,48:58-65. [7]AHMED A S,SNCHEZ C P,CANDELA M E.Evaluation of induction of systemic resistance in pepper plants (Capsicum annuum) to Phytophthora capsici using Trichoderma harzianum and its relation with capsidiol accumulation[J].European Journal of Plant Pathology,2000,106(9):817-824. [8]张爱民,韩世玉,杨红,等.辣椒疫霉菌拮抗木霉菌株的分离与初步筛选[J].贵州农业科学,2014,42(6):87-90. [9]肖淑芹,薛春生,曹远银.辣椒疫霉菌拮抗木霉的筛选及抑菌机制研究[J].北方园艺,2011(5):26-28. [10]陈卫辉,王慧中.哈茨木霉液体培养技术研究[J].江西农业大学学报,1998,20(2):170-174. [11]中华人民共和国国家标准 农药田间药效试验准则(一)[S].北京:中国标准出版社,2000:133-135. [12]KLEIN D,EVELEIGH D E.In:KUBICEK C P,HARMAN G E. Trichoderma and Gliocladium Vol.1[M].London:Taylor and Francis,1998:57-69. [13]BROTMAN Y,KAPUGANTI J G,VITERBO A.Trichoderma[J].Current Biology,2010,20(9):390-391. [14]JAKLITSCH W M.European species of Hypocrea Part I.The green-spored species[J].Studies in Mycology,2009,63:1-91. [15]JAKLITSCH W M.European species of Hypocrea part II:species with hyaline ascospores[J].Fungal Diversity,2011,48(1):1-250. [16]SMITH V L,WILCOX W F,HARMAN G E.Potential for biological control of Phytophthora root and crown rots of apple by Trichoderma and Gliocladium spp.[J].Phytopathology,1990,80:880-885. [17]杨峻,林荣华,袁善奎,等.我国生物源农药产业现状调研及分析[J].中国生物防治学报,2014,30(4):441-445. [18]黄青春,叶钟音.烯酰吗啉(DMM)的特性及其作用机制[J].农药科学与管理,2000,21(5):28-31. [19]崔晓岚,孟庆晓,毕扬,等.辣椒疫霉对烯酰吗啉的敏感性基线及室内抗药突变体研究[J].植物病理学报,2009,39(6):630-637. [20]WINDHAM M T,ELAD Y,BAKER R.A mechanism for increased plant growth induced by Trichoderma spp.[J].Phytopathology,1986,76(5):518-521. [21]SALAS-MARINA M A,SILVA-FLORES M A,URESTI-RIVERA E E,et al.Colonization of Arabidopsis roots by Trichoderma atroviride promotes growth and enhances systemic disease resistance through jasmonic acid/ethylene and salicylic acid pathways[J].European Journal of Plant Pathology,2011,131(1):15-26. [22]REGLIN〖KG-1.8mm〗′SKI T,RODENBURG N,TAYLOR J T,et al.Trichoderma atroviride promotes growth and enhances systemic resistance to Diplodia pinea in radiata pine (Pinus radiata) seedlings[J].Forest Pathology,2012,42(1):75-78. [23]RABEENDRAN N,JONES E E,MOOT D J,et al.Biocontrol of Sclerotinia lettuce drop by Coniothyrium minitans and Trichoderma hamatum[J].Biological Control,2006,39(3):352-362. [24]EL-HASSAN S A,GOWEN S R,PEMBROKE B.Use of Trichoderma hamatum for biocontrol of lentil vascular wilt disease:Efficacy,mechanisms of interaction and future prospects[J].Journal of Plant Protection Research,2013,53(1):12-26.
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备注/Memo
第一作者简介:席亚东(1981-),男,硕士研究生,研究方向为蔬菜病害综合防治。E-mail:xiyadong2002@126.com.责任作者:陈国华(1979-),女,博士,副研究员,研究方向为蔬菜病害综合防治。E-mail:chenguohua01@caas.cn.基金项目:公益性行业(农业)科研专项资助项目(201303014);四川省财政厅“蔬菜品种抗病性评价与病虫害绿色防控技术”资助项目(ysny-1);国家产业体系四川省蔬菜产业体系资助项目(nycytx-31);四川省科技支撑计划资助项目(2016NZ0032)。