JIAN Liru,DU Shuangtian.Effect of Nitrogen and Inorganic Salt on Production of Stroma,Cordycepin and Adenosine in Cordyceps militaris[J].Northern Horticulture,2016,40(19):163-167.[doi:10.11937/bfyy.201619041]
氮源和无机盐对蛹虫草子座产量及虫草素和腺苷的影响
- Title:
- Effect of Nitrogen and Inorganic Salt on Production of Stroma,Cordycepin and Adenosine in Cordyceps militaris
- Keywords:
- Cordyceps militaris; nitrogen resource; inorganic salt; cordycepin; adenosine
- 文献标志码:
- A
- 摘要:
- 以小麦为主要栽培基质,通过添加不同的氮源和无机盐,选用优质蛹虫草菌株CM-16进行人工培养,探索这2个因素对蛹虫草子座产量及虫草素和腺苷的影响。结果表明:栽培过程中选用6 g?L-1的蛋白胨作为最佳氮源,子座干样质量、虫草素和腺苷的生成量均较高,分别为43.1 g?盒-1、3.95 mg?g-1和2.35 mg?g-1。无机盐则选择1.0 mg?L-1的硫酸亚铁最为合适,此时子座干样质量、虫草素和腺苷的生成量分别为48.8 g?盒-1、4.98 mg?g-1和2.10 mg?g-1。
- Abstract:
- Wheat was the main culture medium in which different kinds of nitrogen resource and inorganic salt were added.High quality Cordyceps militaris CM-16 was cultured in wheat medium and the effects of 2 factors on yield of stroma,cordycepin and adenosine was studied.The results showed that 6 g?L-1 peptone was optimal nitrogen source in cultivation.The production of stoma,cordycepin and adenosine were 43.1 g?box-1,3.95 mg?g-1 and 2.35 mg?g-1 respectively.The optimal inorganic salt was 1.0 mg?L-1 ferrous sulfate.The production of stoma,cordycepin and adenosine were 48.8 g?box-1,4.98 mg?g-1 and 2.10 mg?g-1 under this cultivation conditions.
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备注/Memo
第一作者简介:简利茹(1975-),女,硕士,实验师,现主要从事食用菌栽培等研究工作。E-mail:liru75@nwsuaf.edu.cn.责任作者:杜双田(1961-),男,本科,副教授,现主要从事食用与药用真菌等研究工作。E-mail:dst107@126.com.基金项目:陕西省科技统筹创新工程计划资助项目(2011KTCL02-16)。