FANG Xiaocui,QU Heli,ZHAO Sifeng,et al.Pathogen Identification of Processing Chili Pepper Root Rot Disease in Xinjiang[J].Northern Horticulture,2016,40(15):108-112.[doi:10.11937/bfyy.201615027]
新疆加工辣椒根腐病病原鉴定
- Title:
- Pathogen Identification of Processing Chili Pepper Root Rot Disease in Xinjiang
- 文献标志码:
- A
- 摘要:
- 以制干辣椒根腐病病样为分离对象，采用形态学和分子生物学方法对致病菌进行鉴定。结果表明：共采集根腐病样211个，从中分离获得161个分离物，其中镰刀菌Fusarium spp.121个，占总分离物的75.16%;其它菌40个，占24.84%，说明镰刀菌是新疆加工辣椒的主要致病菌。经致病性测定后，采用形态学观察结合18S rDNA-ITS、EF-1α和β-tubulin 3个序列分析，将80株有致病性的镰刀菌鉴定为尖孢镰刀菌(F.oxysporum)和茄病镰刀菌(F.solani)，其中尖孢镰刀菌57株，占镰刀菌分离物的47.11%;茄病镰刀菌23株，占19.01%。
- Abstract:
- To isolate and identify pathogen of processing chili pepper root rot disease in Xinjiang.A total of 211 samples were collected from different main production areas in Xinjiang.The pathogens which caused root rot of processing chili pepper were isolated and Fusarium isolates were identified according to the morphological features of culture and 18S rDNA-ITS,EF-1α,β-tubulin gene sequences analysised of representative strains.The results showed that 211 samples of root rot diseased chili pepper plant were collected and 161 isolates were obtained.121 of these，75.16% of the total isolates，belonged to Fusarium which were the main pathogen for root rot of processing chili pepper in Xinjiang.80 isolates with pathogenicity were identified to class into two different species，e.g.Fusarium oxysporum and Fusarium solani.Fusarium oxysporum were 57 strains，accounted for 47.11%，Fusarium solani were 23 strains，accounted for 19.01%.
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备注/Memo
第一作者简介：方晓翠(1990-)，女，硕士研究生，研究方向为植物菌物病害及其防治。E-mail：1187343513@qq.com.责任作者：赵思峰（1975-），男，博士，教授，研究方向为植物病害生物防治。E-mail:zhsf_agr@shzu.edu.cn.基金项目：新疆生产建设兵团科技支疆专项资助项目（2014AB019）。