SHAO Yuanjian,WU Wenwen.Genetic Analysis on Pollen Fertility of a Male Sterility Line 3198A in Pepper[J].Northern Horticulture,2016,40(15):27-30.[doi:10.11937/bfyy.201615006]
辣椒不育系3198A雄性不育性状的遗传分析
- Title:
- Genetic Analysis on Pollen Fertility of a Male Sterility Line 3198A in Pepper
- 文献标志码:
- A
- 摘要:
- 辣椒（Capsicum annuum L.）是我国第二大蔬菜作物，而雄性不育性状是辣椒杂交种选育中最重要的性状之一。该研究利用辣椒不育系3198A和恢复系3414-3-5的杂交后代F2分离群体249个植株，在观察花药形态的基础上，对花粉育性的分离情况进行了遗传分析。结果表明：不育系、恢复系、保持系三者的花药形态存在明显的差异，不育系的花药瘦小无花粉，恢复系、保持系的花药大而饱满，成熟花粉中充满淀粉粒；F2代花粉育性分离结果分析表明，辣椒雄性不育系3198A的雄性不育性状受到3对隐性核基因的控制，且存在光温敏不育基因。该研究结果将有助于进一步研究辣椒光温敏雄性不育的遗传特性，分离并选育出光温敏不育系，加速辣椒杂交种的推出。
- Abstract:
- Pepper (Capsicum annuum L.) is the second important vegetable in China.Male sterility is one of the most important traits in hybrid pepper breeding.In this study，the anther morphology of three lines，sterile line，maintainer line and restorer line were observed.And genetic analysis of the separation of pollen fertility was conducted in a F2 population with 249 lines derived from the cross between 9108A (a sterile line) and 3414-3-5 (a restorer line).Significant difference of anther morphology was observed among three lines.The anther of sterile line was small and shrunken with no pollen，whereas the anther of maintainer line and restorer line was bigger with abundant pollens and the matured pollens were full of starch.In addition，based on the analysis of the ratio of fertility segregation in F2 population，three genic recessive genes were responsible for the male sterile，of them，a photo-thermo sensitive gene existed.The observations would facilitate the further study on the photo-thermo sensitive characteristics，to isolate and select PTGMS breeding lines，and thereby to accelerate the breeding process of pepper hybrids.
参考文献/References:
[1]邹学校,周群初,戴雄泽,等.辣椒雄性不育系9704A的选育[J].湖南农业科学,2000(5):39-40. [2]刘金兵,孙洁波,王述彬,等.中熟辣椒新品种江蔬3号的选育[J].中国辣椒,2001(2):26-27. [3]常绍东,黄贞,黄邦海,等.早熟辣椒新品种辣优2号的选育[J].中国蔬菜,2002(1):22-23. [4]范妍芹,刘云.甜椒雄性不育两用系一代杂种‘冀研6号’[J].园艺学报,2002，29(3):101. [5]耿三省,梁红军.优质出口型干鲜两用辣椒新品种-京辣2号[J].蔬菜,2007(11):16-17. [6]杨凤梅,薛庆华,杨高强,等.辣椒三系杂交种沈研13号选育[J].辣椒杂志,2008(2):13-14. [7]范妍芹,刘云,严立斌,等.利用雄性不育系育成大果型甜椒新品种‘冀研12号’[J].园艺学报,2009，36(12):1845-1846. [8]朱卯芬,刘童光,戴祖云,等.辣椒雄性不育三系品种安辣6号的选育[J].中国瓜菜,2009(1):20-21. [9]耿三省,陈斌,张晓芬,等.我国辣椒育种动态及市场品种分布概况[J].辣椒杂志,2011(3):5-8,13. [10]刘玲,李显日,王广华,等.国内辣椒雄性不育育种及分子生物学研究进展[J].生物技术进展,2011,1(4):254-259. [11]薛玉梅,薛庆华,杨高强.早熟、优质干椒三系杂交种沈研16号的选育[J].吉林蔬菜,2011(2):77-78. [12]MARTIN J A，CRAWFORD J H.Several types of sterility in Capsicum frutescens[J].Proc Amer Soc Hort Sci,1951,57:335-338. [13]邵元健,吴雯雯,沈素香,等.辣椒雄性核不育基因的遗传研究及其在杂交育种中的应用[J].热带亚热带植物学报,2013,21(1):93-99. [14]DASKALOV S，POULOS J M.Updated capsicum gene list[J].Caps and Eggp Newsl,1994(13):16-26. [15]SHIFRISS C.Additional spontaneous male sterile mutant in Capsicum annuum L.[J].Euphytica,1973,22:527-529. [16]SHIFRISS C，FRANKEL R.A new male sterility gene in C.annuum L.[J].J Amer Soc Hortic Sci,1969,94:385-387. [17]SHIFRISS C，RYLSKI I.A male sterile (ms-2) gene in California wonder pepper[J].Hort Sci,1972,7(1):36. [18]DASKALOV S.A male sterile (Capsicum annuum L.) mutant[J].Theor Appl Genet,1968,38:370-372. [19]DASKALOV S.Two new male sterile mutants by pepper (C.annum)[J].C R Acad Sci Agr Bulg,1971(4):291-294. [20]DASKALOV S.Investigation of induced mutants in Capsium annuum L.III.Mutants in the variety Zlaten Medal[J].Genet Plant Breeding,1973(6):419-429. [21]POCHARD E.Induction of three male sterility mutations in red pepper (C. annuum L.) by mutagenic treatment of monoploid material[M].In:La sterilite male chez les plantes hort，EUCARPIA,1970:93-95. [22]MESHRAM L D，CHOUDHARI R V，KUKADE B Y，et al.Functionnal male sterility in hot chilli (Capsicum annuum L.)[C].Procs 8th Eucarpia Meeting on Genetics and Breeding on Capsicum and Eggplant，Rome，Italy,1992：7-10 Deppt,61-65. [23]MESHRAM L D，NARKHEDE M N.Natural male sterile mutant in hot chilli[J].Euphytica,1982,31:1003-1005. [24]PATHAK C S，SINGH D P，DESHPANDE A A.Male and female sterility in chilli pepper (Capsicum annuum L.)[J].Capsicum Nswl,1983(2):95-96. [25]KIM H，KIM B D.The organization of mitochodrial atp6 gene region in male fertile and CMS lines of pepper (Capsicum annuum L.)[J].Curr Genet,2006，49:59-67. [26]杨世周.辣椒雄性不育两用系的选育[J].园艺学报,1981,8(3):49-53. [27]杨凤梅,杨世周,姜恩国,等.辣椒AB92雄性不育两用系选育及利用[J].辽宁农业科学,1994(6):15-18. [28]范妍芹,郭景印.甜椒雄性不育系选育初报[J].华北农学报,1994(9):14. [29]袁俊水,李锁平.一个辣椒功能性雄性不育系的花器形态及遗传研究[J].遗传,2000,22(1):29-30. [30]GRZEGORZ B，CEZARY W，PIOTR G,et al.Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on the chromosome P4 using PCR-based markers useful for breeding programmes[J].Euphytica,2012,186:453-461. [31]LEE J，HAN J H，AN C G，et al.A CAPS marker linked to a genic male-sterile gene in the colored sweet pepper，‘Paprika’ (Capsicum annuum L.)[J].Breed Sci,2010,60:93-98.
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备注/Memo
第一作者简介：邵元健(1969-)，男，博士，教授，现主要从事瓜果分子育种等研究工作。E-mail：173482429@qq.com.基金项目：南通市科技创新与产业化资助项目（HL2013019）；江苏省农业科技支撑计划资助项目（BE2011380）；江苏高校品牌专业建设工程资助项目（PPZY2015B174）。