ZHANG Bo,LUO Qing,ZHANG Xiyan,et al.Embryoid Induction of Lycium barbarum L．Anther Culture[J].Northern Horticulture,2016,40(09):105-108.[doi:10.11937/bfyy.201609028]
宁夏枸杞花药培养胚状体的诱导
- Title:
- Embryoid Induction of Lycium barbarum L．Anther Culture
- Keywords:
- Lycium barbarum L.; anther culture; embryoid
- 文献标志码:
- A
- 摘要:
- 以宁夏枸杞新优品系为试材，利用花药培养技术，研究了不同基因型、激素配比、低温预处理、热激处理、添加活性炭对其胚状体诱导率的影响。结果表明：宁夏枸杞新优品系‘06-16’在基因型筛选中，诱导率最高为0.95%，通过对试验体系进行优化，2.0 mg/L KT与0.2 mg/L 2,4-D 的组合诱导率达到2.07%。4℃低温预处理3 d或5 d、33℃热激处理5 d、活性炭浓度为0.8%，均能提高新优品系‘09-106’的胚状体诱导率。
- Abstract:
- Taking Ningxia wolfberry new superior strain as the materials，using anther culture technology,the effect of different genotypes,hormone ratio,cold and hot temperature pretreatment,carbon on embryoid induction from the anther of Ningxia wolfberry was studied.The results showed that the ‘06-16’ was easy to be induced.The medium adding 2.0 mg/L KT and 0.2 mg/L 2,4-D was suitable for the induction of embryoid,the induction rate was 2.07%.When the flower bud was treated 3 days or 5 days under 4℃，cultivated in the high temperature (33℃)，adding 0.8% active carbon in midia，the induced rate could be improved.
参考文献/References:
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备注/Memo
第一作者简介：张波（1984-），女，宁夏中宁人，硕士，研究实习员，现主要从事枸杞遗传育种等研究工作。E-mail:zhang_bo_0309@126.com. 责任作者：曹有龙（1963-），男，博士，研究员，现主要从事枸杞遗传育种及分子生物学等研究工作。E-mail:youlongchk@163.com. 基金项目：宁夏回族自治区自然科学基金资助项目(NZ13126);国家科技厅枸杞育种专项资助项目（2013NYYZ0105）；宁夏农林科学院自主研发资助项目(NKYJ-14-08)。