ZHONG Fangli,WANG Wenjiao,WANG Xiaolin,et al.Study on Extraction Process of Total Saponins in Platycodon grandiflorum Stems With Aqueous Two-phase Cooperated With Microwave Method and Its Antioxidant Ability[J].Northern Horticulture,2016,40(07):119-124.[doi:10.11937/bfyy.201607029]
微波协同双水相萃取桔梗茎总皂苷及其抗氧化活性研究
- Title:
- Study on Extraction Process of Total Saponins in Platycodon grandiflorum Stems With Aqueous Two-phase Cooperated With Microwave Method and Its Antioxidant Ability
- 文献标志码:
- A
- 摘要:
- 以桔梗茎为试材，采用微波协同双水相的方法，研究了各因素对桔梗茎总皂苷萃取率的影响，并考察了桔梗茎总皂苷提取液的体外抗氧化活性。结果表明：微波协同双水相萃取桔梗茎总皂苷的最佳工艺条件为料液比1∶25 g/mL，微波功率600 W，醇水比（mL∶mL）0.77，萃取时间7 min，硫酸铵质量浓度0.21 g/mL，在此工艺条件下，其平均萃取率为92.31%；桔梗茎总皂苷提取液具有一定的抗氧化能力，而且其抗氧化活性随桔梗茎总皂苷提取液质量浓度的增加而逐渐提高。
- Abstract:
- Using Platycodon grandiflorum stems as experimental material，aqueous two-phase assisted with microwave method was applied to explore the influence of many factors on the total saponins extraction efficiency and its antioxidant ability in vitro.The results showed that the optimum ratio between material and solvent，the microwave power，the ratio between ethanol and water，extraction time and the mass concentration of ammonium sulfate were 1∶25 g/mL，600 W，0.77，7 min and 0.21 g/mL,respectively.Under optimum process condition，the average total saponins extraction efficiency reached 92.31%.The activity test showed that total saponins extraction solution from Platycodon grandiflorum stems had certain antioxidant ability in vitro，which might gradually enhance the clearance ratio with the increase of mass concentration of total saponins in extraction solution.
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备注/Memo
第一作者简介：钟方丽（1970-），女，山东安丘人，博士，教授，现主要从事天然产物化学成分的分离与生物活性等研究工作。E-mail：fanglizhong@sina.com. 责任作者：王晓林(1969-)，男，山东五莲人，硕士，副教授，现主要从事天然产物有效成分的提取及纯化工艺等研究工作。E-mail：wangxiaolin69@eyou.com.