FANG He,SONG Wangdi,CHEN Wen.Purification of Total Flavonoids From the Flowers of Salix caprea L.by the Macroporous Adsorption Resin[J].Northern Horticulture,2016,40(06):120-124.[doi:10.11937/bfyy.201606030]
大孔吸附树脂纯化黄花柳花总黄酮工艺研究
- Title:
- Purification of Total Flavonoids From the Flowers of Salix caprea L.by the Macroporous Adsorption Resin
- 文献标志码:
- A
- 摘要:
- 以黄花柳花为试材，采用紫外分光光度法，选择5种不同型号的树脂进行静态吸附试验，筛选出对其吸附解吸效性能高的树脂，再通过动态吸附与解吸试验研究上样浓度、树脂上样量、洗脱剂及流速等条件对黄花柳花总黄酮纯化效果的影响，优化维药黄花柳花总黄酮的大孔树脂纯化工艺。结果表明：AB-8型大孔吸附树脂对黄花柳花总黄酮具有较好的吸附解吸效果，最佳纯化条件为黄花柳花待纯化液浓度为3.13 g/L，调节上样液pH 4.2，上样量5.5 mL/g，以 1.0 mL/min流速上样，依次用10 BV蒸馏水淋洗，6 BV 70%乙醇以2.0 mL/min流速洗脱，得纯化液。经AB-8纯化后的黄花柳花总黄酮纯度达51.99%。AB-8型大孔吸附树脂能有效提高黄花柳花中总黄酮纯度，并符合中药有效部位研究要求。
- Abstract:
- Taking Salix caprea L.as material，the optimum technological parameters of the purification process of total flavones from Salix caprea L.flowers with macroreticular resins was explored.The contents of total flavonoids were determined by using UV-Vis spectrum.Five kinds of resins were examined by static test.Purification conditions were investigated by using dynamic adsorption and desorption experiment.The results showed that AB-8 macroporus resin had good adsorption and desorption properties for favonoids in Salix caprea flowers.The optimal refined conditions were as follows：the velocity of sample solution 1.0 mL/min and pH 4.2，sample concentration of 3.13 g/L，the loaded amount of 5.5 mL/g，washing with distilled water 10 BV，then eluted with 6 BV of 70% ethanol at 2.0 mL/min.Under those optimum conditions，the purity of total flavonoids and the recovery was up to 51.99%.AB-8 macroreticular absorptive resin could effectively separate and purify total flavones from Salix caprea flowers，the purity coefficient thus was over 50%，which was in line with the study of effective components of herbal medicine.
参考文献/References:
[1]何江，徐芳，陈燕，等.维吾尔药材黄皮柳花与其混淆品黄花柳花的鉴别研究[J].时珍国医国药，2010，21(8):1997-1998. [2]居来提·托乎提，吐洪·艾买尔，吴秋灵.维吾尔医药爱维心口服液治疗冠心病心绞痛临床疗效观察试验研究[J].中国民族医药杂志，2009，4(4)：10-13. [3]中国医学百科学编辑委员会.中国医学百科书(维吾尔医学)[M].上海:上海科学技术出版社，2005:210-211. [4]ALAM M S，KAUR G，JABBAR Z，et al.Evaluation of antioxidant activity of Salix caprea flowers[J].Phytother Res，2006，20(6):479-483. [5]TUNON H，OLAVSDOTTER C，BOHLIN L.Evaluation of anti- inflammatory activity of some swedish medicinal plants inhibition of prostaglandin biosynthesis and PAF-induced exocytosis[J].J Ethnopharmacol，1995，48(2)：61-76. [6]SULTANA S，SALEEM M.Salix caprea inhibits skin carcinogenesis in murine skin：inhibition of oxidative stress，ornithine decarboxylase activity and DNA synthesis[J].Journal of Ethnopharmacology，2004，91(2)：267-276. [7]AHMED A，SHAH WA，AKBAR S，et al.A short chemical review on Salix caprea commonly known as goa willow[J].Int J Res Phytochem Pharmacol，2011，1(1)：17-20. [8]孙慧丽，艾来提·苏里坦，买吾兰·买提努尔，等.黄花柳中总黄酮含量的研究[J].新疆农业科学，2008，45(1):84-87. [9]买吾兰江·买提努尔，艾来提·苏里坦，夏热帕提，等.响应面分析法优化超声波提取黄花柳总黄酮工艺[J].新疆农业科学，2009，46(4)：867-872. [10]陈小川，冉继春，蒋瑶.大孔吸附树脂在中草药分离纯化中的应用[J].重庆中草药研究，2014(1):27-30. [11]程振玉，宋海燕，杨英杰，等.AB-8大孔吸附树脂静态纯化北五味子木脂素工艺研究[J].北方园艺，2015(7)：141-145. [12]闵建华，章振鹏，孟亚会，等.红景天苷大孔吸附树脂纯化工艺研究[J].中草药，2013，44(11)：1426-1430. [13]柴建新，万茵，付桂明，等.杜仲叶总黄酮含量测定方法优化[J].中国食品学报，2013，13(4)：225-230. [14]杨艳俊，王亚红，王君龙.景天三七总黄酮大孔吸附树脂纯化工艺研究[J].北方园艺，2014(7)：144-147. [15]朱梦良，招丽君，梁新丽，等.大孔吸附树脂分离纯化葛根总黄酮和葛根素的工艺优选[J].中国实验方剂学杂志，2013，19(4)：23-26.
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备注/Memo
第一作者简介：方贺(1990-)，女，硕士研究生，研究方向为药物新剂型研究。E-mail:358831940@qq.com.责任作者：陈文(1967-)，男，博士，教授，研究方向为药物新剂型研究。E-mail:chen-wen2000@126.com.基金项目：国家“重大新药创制”科技重大专项资助项目（2011ZX09401-007）。