YU Xiaomin,WU Lei,WANG Kui,et al.Study on Genetic Transformation in Radish by Agrobacterium tumefaciens[J].Northern Horticulture,2016,40(06):99-102.[doi:10.11937/bfyy.201606024]
根癌农杆菌介导的萝卜遗传转化方法研究
- Title:
- Study on Genetic Transformation in Radish by Agrobacterium tumefaciens
- Keywords:
- transgenic; Agrobacterium tumefaciens; radish; floral dip method
- 文献标志码:
- A
- 摘要:
- 以“短叶-13”萝卜为试材，采用浸花法，使用含有绿色荧光蛋白（GFP）报告基因的根癌农杆菌浸泡生长状态良好的萝卜花序，研究了农杆菌介导萝卜遗传转化的新方法。结果表明：GFP蛋白能够在萝卜幼根中成功表达。
- Abstract:
- Taking radish ‘Short leaf-13’ as material，using dipping flower method，a new method of Agrobacterium tumefaciens for genetic transformation in radish (Raphanus sativus L.)were researched.The results showed that GFP could successfully be expressed in the root of radish.
参考文献/References:
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备注/Memo
第一作者简介：喻晓敏（1984-），女，硕士，编辑，现主要从事植物生物技术及出版学等研究工作。E-mail:21581872@qq.com.责任作者：李世升（1983-），男，博士，讲师，现主要从事作物发育与育种等研究工作。E-mail:lishisheng2002@whu.edu.cn.基金项目：湖北省自然科学基金资助项目（2013CFC126）；湖北省教育厅自然科学研究资助项目（D20122703）。