ZHAO Huan,ZHANG Li.Establishment of the System of Rapid Propagation and Bud Induction of Fittonia[J].Northern Horticulture,2016,40(01):87-89.[doi:10.11937/bfyy.201601023]
网纹草丛芽诱导与组培快繁体系的建立
- Title:
- Establishment of the System of Rapid Propagation and Bud Induction of Fittonia
- Keywords:
- textured grass; stems; buds proliferation; tissue culture
- 文献标志码:
- A
- 摘要:
- 以网纹草“小火焰”、“白天使”2个品种的茎尖和茎段为外植体，研究了不同消毒时间、不同碳源、不同配比的生长调节剂对网纹草丛芽诱导、增殖和生根的影响。结果表明：外植体灭菌15 min时污染率最低，“小火焰”、“白天使”茎段污染率分别为16.7%和13.3%。“白天使”品种最佳丛芽诱导培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，最佳继代培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L。“小火焰” 品种最佳丛芽诱导培养基为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L，最佳继代培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，继代培养基中将蔗糖换成白砂糖效果更佳。2个品种的生根培养基均以1/2MS+IBA 0.1 mg/L效果较好。
- Abstract:
- With fittonia verschaffeltii ‘Small flame’,‘White angel’ two varieties of shoot tips and stem segments as explants，the effect of different disinfection time，different C source，and proportion of growth regulators on explants was researched.The results showed that the contamination rate was the lowest after explants sterilization 15 minutes，and the ‘Small flame’，‘White angel’ stem segments contamination rate were 16.7% and 13.3%，respectively.The best buds induction medium of the ‘White angel’ was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L，the optimal subculture medium was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.The better variety buds induced culture of ‘Small flame’ was MS+6-BA 2.0 mg/L+NAA 0.1 mg/L，and the best performance could be seen when the subculture medium was MS+6-BA 1.0 mg/L+NAA 0.2 mg/L.Following in the sucrose medium was replaced with sugar better.Two varieties performed well when the rooting medium was 1/2MS+IBA 0.1 mg/L.
参考文献/References:
[1]DEWOLF G P.The genus fittonia (Acanthaceae) [J].Baileya,1970,17:34-37. [2]BRUMMITT R K.Proposal(447) to conserve the name 8069 fittonia coemans over adelaster lndleyex veitch(Acanthaceae) [J].Taxon,1978（2）:307-309. [3]陈超,王桂兰,石洪凌,等.白网纹草的化学诱变与快速繁殖[J].植物生理学通讯,2003,39(6):631. [4]傅婉华,李文安.白网纹草的茎尖培养和快速繁殖[J].植物生理学通讯,1988(1):46. [5]陈志红,李华赐.白网纹草试管苗的诱导[J].植物生理学通讯,1987(3):37-38. [6]杨承勇,郑迎冬.白网纹草的茎段培养和快速繁殖[J].仲恺农业技术学院学报,2000,13(1):15-18.
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备注/Memo
第一作者简介：赵欢(1992-)，女,硕士研究生，研究方向为园林植物与观赏园艺。E-mail:836986582@qq.com.责任作者：张黎（1962-），女，硕士，教授，硕士生导师，现主要从事园林植物与观赏园艺等研究工作。E-mail:zhang_li9988@163.com.基金项目：宁夏科技支撑计划资助项目(2014ZZN09)。