ZHANG Xiangyu,CHEN Jie,JI Yun,et al.Study on Bulbil Induction Callus Differentiation From Cluster Buds of Lilium sulphureum[J].Northern Horticulture,2015,39(19):101-105.[doi:10.11937/bfyy.201519025]
淡黄花百合珠芽诱导愈伤组织 再分化出丛生芽的研究
- Title:
- Study on Bulbil Induction Callus Differentiation From Cluster Buds of Lilium sulphureum
- Keywords:
- Lilium sulphureum; bulbil; callus; differentiation; cluster buds
- 文献标志码:
- A
- 摘要:
- 以淡黄花百合珠芽为试材，采用植物组织培养方法，研究不同浓度的TDZ、NAA、2,4-D、ZT对淡黄花百合珠芽愈伤组织诱导及再分化出丛生芽的影响。结果表明：MS+0.3 mg/L TDZ+0.5 mg/L NAA+1.0 mg/L 2,4-D+0.5 mg/L ZT为最佳培养基，最快可在15 d诱导出愈伤组织，愈伤组织诱导率为（98.67±2.31）%，最快可在32 d分化出丛生芽，丛生芽诱导率为（98.67±2.31）%，平均出芽数为（17.67±0.58）个。
- Abstract:
- The Lilium sulphureum callus were used as material by adopting the method of plant tissue culture，the effect of different concentrations of TDZ，NAA，2，4-D，ZT on bulbil induction callus differentiation from cluster buds of Lilium sulphureum was studied.The results showed that MS+0.3 mg/L TDZ+0.5 mg/L NAA+1.0 mg/L 2,4-D+0.5 mg/L ZT was the optimum medium,callus appeared in the fifteenth days,induction rate was (98.67±2.31)% of callus,cluster buds differentiated in the thirty-second days,induction rate was (98.67±2.31)% of cluster buds,average budding was (17.67±0.58)%.
参考文献/References:
[1]刘德军,黄跃进.百合[M].北京:中国中医药出版社，2001. [2]杨晶,商万有.百合的药用价值研究[J].吉林农业，2011(7):271. [3]周春华,尤超,陈凝华.百合组织培养研究进展[J].北方园艺，2013(14):193-195. [4]袁丽丽,刘青林.从第二届国际百合属研讨会看世界百合研究趋势[J].中国花卉园艺，2010(22):13-15. [5]杨雪清,王淑芳,杨蕊,等.淡黄花百合丛生小鳞茎的诱导及快速繁殖[J].河南农业科学，2007(3):98-100. [6]李黛,谈锋,祝顺琴.淡黄花百合的组织培养[J].种子，2005,24(9):27-29. [7]邱宁宏,罗林会,王勤，等.淡黄花百合的组织培养与快速繁殖[J].中国野生植物资源，2004,23(2):64-65. [8]徐晓峰,黄学林.TDZ：一种有效的植物生长调节剂[J].植物学通报，2003(20)：227-237. [9]陈云凤,张春荣,黄霞，等.TDZ对植物体细胞胚胎发生的作用[J].植物生理学通讯，2006,42(1):127-133. [10]VICTOR J M R，MURTHY B N S，MURCH S J，et al.Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut （Arachis hypogaea L.)[J].Plant Growth Regul，1996,28：41-47. [11]HUTCHINSON M J，SAXENA P K.Acetylsalicylic acid enhances and synchronizes thidiazuron-induced somatic embryogenesis in geranium (Pelargonium×hortorum Bailey) tissue cultures[J].Plant Cell Rep，1996,15：512-515. [12]HUTCHINSON M J，SAXENA P K.Role of purine metabolism in thiadiazuron-induced somatic embryogenesis of geranium(Pelargonium×hortorum) hypocotyl cultures[J].Physiol Plant，1996,98：517-522. [13]HUTCHINSON M J，KRISHARAJ S，Saxena P K.Inhibitory effect of GA3 on the development of TDZ-induced somatic embryogenesis of geranium (Pelargonium×hortorum) hypocotyles cultures[J].Plant Cell Rep，1997,16：435-438. [14]HUTCHINSON M J，MURR D P，KRISHARAJ S.Does ethylene play a role in TDZ-regulated somatic embryogenesis of geranium (Pelargonium×hortorum) hypocotyles cultures[J].In vitro Cell Deve Biol-Plant，1997,33：136-141. [15]赵银萍,张九东,程建国.用百合叶片及其鳞茎叶片离体诱导不定芽[J].西安文理学院学报，2005,8(1):21-23.
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备注/Memo
第一作者简介：张翔宇（1986-），男，硕士，助理研究员，现主要从事药用植物资源工程等研究工作。E-mail：304626335@qq.com.基金项目：国家科技支撑计划子课题资助项目（2015BAI05B00）；毕节市成果转化资助项目（[毕科成字(2014)8]号）；贵州省中药现代化科技产业研究开发专项资助项目（黔科合SY字（2014）3078-5号）。