DING Ling,LI Chonghui,HUANG Shaohua,et al.Research Advances of Flavonoid 3′-Hydroxylase Gene in Ornamental Plant[J].Northern Horticulture,2015,39(17):188-193.[doi:10.11937/bfyy.201517049]
调控观赏植物花色的类黄酮3′羟化酶基因研究进展
- Title:
- Research Advances of Flavonoid 3′-Hydroxylase Gene in Ornamental Plant
- 文献标志码:
- A
- 摘要:
- 综述了类黄酮3′羟化酶基因(F3′H)调控观赏植物花色的分子机理及近年来的研究进展,总结了类黄酮生物合成途径中F3′H的作用,F3′H基因的分离克隆及鉴定方法、表达模式及其对花色的影响,并提出未来研究的展望,以期为进一步明确 F3′H 基因调控花色的分子机理研究以及可能的花色定向育种提供参考。
- Abstract:
- Research progress in the molecular mechanisms of flavonoid 3′-hydroxylase gene (F3′H) regulating the color of ornamental plants in recent years were reviewed.The role of F3′H in the flavonoid biosynthetic pathway,the methods of gene isolation,cloning,identification,expression patterns and the impact of F3′H on floral color were summarized.Future research were proposed,in order to provide reference for further understanding of the molecular mechanism of F3′H gene and for desirable color breeding.
参考文献/References:
[1]TANAKA Y,SASAKI N,OHMIYA A.Plant pigments for coloration:anthocyanins,betalains and carotenoids[J].Plant J,2008,54(4):733-749. [2]GRAF B A,MILBURY P E,BLUMBERG J B.Flavonols,flavones,flavanones,and human health:epidemiological evidence[J].J Med Food,2005,8(3):281-290. [3]HARBORNE J B,WILLIAMS C A.Advances in flavonoid research since 1992[J].Phytochemistry,2000,55(6):481-504. [4]FORKMANN G,HELLER W.Comprehensive natural products chemistry[M].Amsterdam:Elsevier,1999:713-748. [5]KEYSER E D,DESMET L,BOCKSTAELE E V,et al.How to perform RT-qPCR accurately in plant species?A case study on flower colour gene expression in an azalea(Rhododendron simsii hybrids)mapping population[J].BMC Molecular Biology,2013,14(1):13. [6]OHNO S,HOSOKAWA M,HOSHINO A,et al.A bHLH transcription factor,DvIVS,is involved in regulation of anthocyanin synthesis in dahlia(Dahlia variabilis)[J].Journal of Experimental Botany,2011,62(14):5105-5116. [7]GOTO T,KONDO T.Structure and molecular stacking of anthocyanins-flower color variation[J].Angew Chem,1991,30(1):17-33. [8]SCHLANGEN K,MIOSIC S,HALBWIRTH H.Allelic variants from Dahlia variabilis encode flavonoid 3′-hydroxylases with functional differences in chalcone 3-hydroxylase activity[J].Archives of Biochemistry and Biophysics,2010,494(1):40-45. [9]NELSON D R,SCHULER M A,PAQUETTE S M,et al.Comparative genomics of rice and Arabidopsis analysis of cytochrome P450 genes and pseudogenes from a monocot and a dicot[J].Plant Physiol,2004,35(2):756-772. [10]BOASE M R,LEWIS D H,DAVIES K M,et al.Isolation and antisense suppression of flavonoid 3′,5′-hydroxylase modifies flower pigments and colour in Cyclamen[J].BMC Plant Biology,2010,10:107. [11]DES MARAIS D L,RAUSHER M D.Parallel evolution at multiple levels in the origin of humming bird pollinated flowers in Ipomoea[J].Evolution,2010,64(7):2044-2054. [12]贾赵东,马佩勇,边小峰,等.植物花青素合成代谢途径及其分子调控[J].西北植物学报,2014,34(7):1496-1506. [13]BRUGLIERA F,REWELL G B,TIMOTHY A,et al.Isolation and characterization of a flavonoid 3′-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida[J].The Plant Journal,1999,19(4):441-451. [14]YUAN Y,MA X H,SHI Y M,et al.Isolation and expression analysis of six putative structural genes involved in anthocyanin biosynthesis in Tulipa fosteriana[J].Scientia Horticulturae,2013,153(4):93-102. [15]杨玉霞,孙菲菲,张昌伟.蝴蝶兰全长cDNA文库构建及F3′H基因克隆[J].西北植物学报,2013,33(9):1731-1738. [16]LAI Y S,SHIMOYAMADA Y,NAKAYAMA M,et al.Pigment accumulation and transcription of LhMYB12 and anthocyanin biosynthesis genes during flower development in the Asiatic hybrid lily(Lilium spp.)[J].Plant Science,2012,193-194:136-147. [17]ZHAO D Q,TAO J,HAN C X,et al.Flower color diversity revealed by differential expression of flavonoid biosynthetic genes and flavonoid accumulationin herbaceous peony (Paeonia lactiflora Pall.)[J].Mol Biol Rep,2012,39(12):11263-11275. [18]ISHIGURO K,TANIGUCHI M,TANAKA Y.Functional analysis of Antirrhinum kelloggii flavonoid 3′-hydroxylase and flavonoid 3′,5′-hydroxylase genes;critical role in flower color and evolution in the genus Antirrhinum[J].J Plant Res,2012,125(3):451-456. [19]SCHLANGEN K,MIOSIC S,THILL J,et al.Cloning,functional expression,and characterization of a chalcone 3-hydroxylase from Cosmos sulphureus[J].Journal of Experimental Botany,2010b,61(12):3451-3459. [20]SCHLANGEN K,MIOSIC S,TOPUZ F,et al.Chalcone 3-hydroxylation is not a general property of flavonoid 3′-hydroxylase[J].Plant Science,2009,177(2):97-102. [21]NAKASUKAA A,MIZUTAB D,KII Y,et al.Isolation and expression analysis of flavonoid biosynthesis genes inevergreen azalea[J].Scientia Horticulturae,2008,118(4):314-320. [22]SEITZ C,EDER C,DEIM B,et al.Cloning,functional identification and sequence analysis of flavonoid 3′-hydroxylase and flavonoid 3′,5′-hydroxylase cDNAs reveals independent evolution of flavonoid 3′,5′-hydroxylase in the Asteraceae family[J].Plant Molecular Biology,2006,61(3):365-381. [23]UEYAMA Y,SUZUKI K,MIZUTANI M F,et al.Molecular and biochemical characterization of torenia flavonoid 3′-hydroxylaseand flavone synthase II and modification of flower color by modulating the expression of these genes[J].Plant Sci,2002,163:253-263. [24]YUAN Y,MA X H,TANG D Q,et al.Comparison of anthocyanin components,expression of anthocyanin biosynthetic structural genes,and TfF3′H1 sequences between Tulipa fosteriana‘Albert heijn’and its reddish sport[J].Scientia Horticulturae,2014,175(15):16-26. [25]HE H,HU K,HAN K T,et al.Flower colour modification of chrysanthemum by suppression of F3′H and overexpression of the exogenous Senecio cruentus F3′5′H gene[J].Plos One,2013,8(11):e74395. [26]BRUGLIERA F,TAO G Q,TEMS U,et al.Violet-blue Chrysanthemums-metabolic engineering of anthocyanin biosynthetic pathway results in novel petal colours[J].Plant and Cell Physiology,2013,54(10):1696-1710. [27]TSUDA S,FUKUI Y,NAKAMURA N,et al.Flower color modification of Petunia hybrida commercial varieties by metabolic engineering[J].Plant Biotechnol,2004,21(5):377-386. [28]NAKAMURA N,MIZUTANI M F,FUKUI Y,et al.Generation of red flower varieties from blue Torenia hybrida by redirection of the flavonoid pathway from delphinidin to pelargonidin[J].Plant Biotechnol,2010,27(5):375-383. [29]HOSHINO A,MORITA Y,CHOI J D,et al.Spontaneous mutations of the flavonoid 3′-hydroxylase gene conferring reddish flowers in the three morning glory species[J].Plant Cell Physiol,2003,44(10):990-1001. [30]ZUFALL R A,RAUSHER M D.Genetic changes associated with floral adaptation restrict future evolutionary potential[J].Nature,2004,428(22):847-850. [31]MARTINS T R,BERG J J,BLINKA S,et al.Precise spatial temporal regulation of the anthocyanin biosynthetic pathway leads to petal spot formation in Clarkia gracilis(Onagraceae)[J].New Phytologist,2013,197(3):958-969. [32]SHIN J,PARK E,CHOI G.PIF3 regulates anthocyanin biosynthesis in an HY5 dependent manner with both factors directly binding anthocyanin biosynthetic gene promoters in Arabidopsis[J].The Plant Journal,2007,49(6):981-994. [33]LAITINEN R A E,AINASOJA M,BROHOLM S K,et al.Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida[J].Journal of Experimental Botany,2008,59(13):3691-3703. [34]NAJATSUKA T,SAITO M,YAMADA E,et al.Isolation and characterization of GtMYBP3 and GtMYBP4,orthologues of R2R3-MYB transcription factors that regulate early flavonoid biosynthesis in gentian flowers[J].Jurnal of Experimental Botany,2012,63(18):6505-6517. [35]HAETMANN U,SAGASSER M,MEHRTENS F,et al.Differential combinatorial interactions of cis-acting elements recognized by R2R3-MYB,BZIP,and BHLH factors control light-responsive and tissue-specific activation of phenylpropanoid biosynthesis genes[J].Plant Molecular Biology,2005,57(2):155-171. [36]胡可,韩科厅,戴思兰.植物花青素合成与基因调控[J].植物学报,2010,45(3):307-317. [37]DELA G,OR E,OVADIA R,et al.Changes in anthocyanin concentration and composition in‘Jaguar’ rose flowers due to transient high-temperature conditions[J].Plant Sci,2003,164(3):333-340. [38]侯杰,佟玲,崔国新,等.植物类黄酮3′-羟化酶(F3′H)基因的研究进展[J].植物生理学报,2011,47(7):641-647.
相似文献/References:
[1]徐 慧,刘 超,钟汉东.不同光照强度对八仙花开花的影响[J].北方园艺,2014,38(01):81.
XU Hui,LIU Chao,ZHONG Han-dong.Effects of Different Light Intensity on the Blossom of Hydrangea macrophylla[J].Northern Horticulture,2014,38(17):81.
[2]唐立红,王志玲,张帆.常用室内观赏植物吸收甲醛能力的研究[J].北方园艺,2014,38(07):62.
TANG Li-hong,WANG Zhi-ling,ZHANG Fan.Research on Formaldehyde of Absorption Abilities Common Indoor Ornamental Plants[J].Northern Horticulture,2014,38(17):62.
[3]宋敏丽.山西历山国家自然保护区菊科野生观赏植物资源[J].北方园艺,2012,36(03):89.
SONG Min-li.Wild Ornamental Compositae Resources in Lishan National Nature Reserve of Shanxi[J].Northern Horticulture,2012,36(17):89.
[4]汪海霞,吴 彤,邢 震,等.西藏蔷薇科观赏植物资源及多样性研究[J].北方园艺,2015,39(11):74.[doi:10.11937/bfyy.201511019]
Biodiversity of Ornamental Plant Rosaceae Resources in Tibet.Biodiversity of Ornamental Plant Rosaceae Resources in Tibet[J].Northern Horticulture,2015,39(17):74.[doi:10.11937/bfyy.201511019]
[5]陈峥,辛丽琴,吴超群,等.VIGS技术在观赏植物中应用的研究进展[J].北方园艺,2016,40(13):196.[doi:10.11937/bfyy.201613050]
CHEN Zheng,XIN Liqin,WU Chaoqun,et al.Advance in Applications of Virus-induced Gene Silencing (VIGS) Technology to Ornamental Plants[J].Northern Horticulture,2016,40(17):196.[doi:10.11937/bfyy.201613050]
[6]石 学 丽,刘 雅 平,罗 言 云.红色系观花植物的科属统计及其园林应用[J].北方园艺,2009,33(10):0.[doi:10.11937/bfyy.2009010070]
SHI Xue -li,LIU Ya -ping,LUO Yan -yun.The Statistics of Families and Classes for Red Flower Plants and the Application in Landscape Architecture[J].Northern Horticulture,2009,33(17):0.[doi:10.11937/bfyy.2009010070]
[7]李 利,韩张雄,徐新文,等.NaCl胁迫对紫穗槐幼苗叶绿素荧光特征的影响[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102029]
LI Li,HAN Zhan~xiong,XU Xirvwe],et al.Effect of NaCI on Chlorophyll Fluorescence Characteristics of Seedlings in Amorpha f ruticosa[J].Northern Horticulture,2011,35(17):0.[doi:10.11937/bfyy.201102029]
[8]田英翠,潘百红,曹受金.八种室内观赏植物对甲醛的净化效果研究[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102031]
TIAN Ying-cui,PPuN Bai-hong,CAO Shou-jin.Research of Eight Kinds of Indoor Foliage Plants to Formaldehyde Purification[J].Northern Horticulture,2011,35(17):0.[doi:10.11937/bfyy.201102031]
[9]吴中军.,张 扬,刘奕清,等.干旱胁迫对琴叶榕生长和生理特性的影响[J].北方园艺,2011,35(03):0.[doi:10.11937/bfyy.201103028]
WU Zhong-jun.,ZHANG Yang,LIU Yi-qing,et al.Effect of Soil Drought Stress on Growth and Physiological Characteristics of F/cus l yrata[J].Northern Horticulture,2011,35(17):0.[doi:10.11937/bfyy.201103028]
[10]王 晓 红,陈 训,陈 月 华,等.贵州百里杜鹃风景区植物景观资源调查与营造建议[J].北方园艺,2008,32(09):0.[doi:10.11937/bfyy.200809057]
W ANG Xiao -hong,CHEN Xun,CHEN Yue -hua,et al.Investigation and Building of Plant Landscape Resources in the One Hundred Mile Azalea Forest in Guizhou[J].Northern Horticulture,2008,32(17):0.[doi:10.11937/bfyy.200809057]
[11]邢世通,韩冰,谷颐.重要观赏植物的花色改良[J].北方园艺,2018,42(09):166.[doi:10.11937/bfyy.20173362]
XING Shitong,HAN Bing,GU Yi.Genetic Improvement of Flower Color in Major Ornamental Plants[J].Northern Horticulture,2018,42(17):166.[doi:10.11937/bfyy.20173362]
备注/Memo
第一作者简介:丁灵(1991-),女,硕士研究生,研究方向为热带花卉生理与分子生物学。E-mail:1060639000@qq.com.责任作者:李崇晖(1983-),女,副研究员,现主要从事热带花卉生理与分子生物学等研究工作。E-mail:blchh@sina.com.基金项目:国家星火计划重大资助项目(2012GA800003);农业公益性行业科研专项资助项目(201203071)。