LI Yanlian,ZHANG Huali,ZHAO Qiang,et al.Study on the One-step Culture of Rapid Propagation Technology in Euryale ferox[J].Northern Horticulture,2015,39(16):107-111.[doi:10.11937/bfyy.201516025]
芡实组培一步成苗技术研究
- Title:
- Study on the One-step Culture of Rapid Propagation Technology in Euryale ferox
- 文献标志码:
- A
- 摘要:
- 以芡实(Euryale ferox)叶片和茎段为外植体,采用MS培养基,附加2,4-D或NAA以及6-BA或KT,根据正交设计的原理,通过因子分析和聚类分析,研究不同激素配比对芡实一步成苗组培快繁的影响。结果表明:在芡实一步成苗快速繁殖中最佳的激素配比是MS+NAA 0.2 mg/L+6-BA 2.0 mg/L,其余依次是MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L、MS+2,4-D 0.2 mg/L+6-BA 1.0 mg/L、MS+2,4-D 1.0 mg/L+KT 1.0 mg/L、MS+NAA 1.5 mg/L+6-BA 1.0 mg/L。
- Abstract:
- Taking Euryale ferox leaves and stem segments as explants,MS as culture medium,the additional 2,4-D or NAA and 6-BA or KT,according to the principle of orthogonal design,using factor analysis and cluster analysis, the effect of different ratio of hormone on rapid propagation of Gordon Euryale step into seedlings was studied.The results showed that the best hormone matchings was MS+NAA 0.2 mg/L+6-BA 2.0 mg/L,others were MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L,MS+2,4-D 0.2 mg/L+6-BA 1.0 mg/L,MS+2,4-D 1.0 mg/L+KT 1.0 mg/L,MS+NAA 1.5 mg/L+6-BA 1.0 mg/L.
参考文献/References:
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备注/Memo
第一作者简介:李彦连(1974-),男,山东济宁人,硕士,副教授,现主要从事植物学的教学与科研工作。E-mail:490174551@qq.com. 基金项目:山东省高等学校科技计划资助项目(J12LE59);济宁学院服务济宁重点资助项目(13FWDF09);济宁市产学研资助项目(2010JNKJ002)。