HAN Hongpeng,SONG Chunpeng.Application Progress of Flow Cytometry in Botany Biology[J].Northern Horticulture,2015,39(15):178-181.[doi:10.11937/bfyy.201515046]
流式细胞术在植物生物学研究中应用进展
- Title:
- Application Progress of Flow Cytometry in Botany Biology
- Keywords:
- flow cytometry; analysis of chromosome ploidy; sorting of protoplasts; in vivo flow cytometry
- 文献标志码:
- A
- 摘要:
- 流式细胞术是一种对细胞基本特性进行快速检测并可根据细胞特性对其进行分类收集的综合性技术。它可以同时定量检测多种物理、生物学参数。目前，该技术已在医药、环保、农业生产、科研等领域广泛应用。作为一种新出现的技术，体内流式细胞术在植物营养、光合产物和代谢产物以及纳米粒子等在植物脉管系统中运输等方面应用前景广阔。在对流式细胞术原理及特点进行介绍的同时，文章对其在植物生物学研究领域及体内流式细胞术的应用进展加以综述。
- Abstract:
- Flow cytometry (FCM) is a comprehensive technology to analyze properties of cells rapidly.FCM can be used to sort cells according their properties.FCM can simultaneously detect various physical and biological parameters quantitatively.At present，FCM is widely applied in medicine，environmental protection，agricultural production，scientific research and other fields.As a new emerging technology，in vivo flow cytometry is a broad application prospects in the transportation of plant nutrients，photosynthetic products and metabolites and nanoparticles in plant vasculature.The principle，characteristics of FCM and its application of research in plant biology were introduced in this paper，and moreover，and the progress in application of in vivo flow cytometry was reviewed.
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备注/Memo
第一作者简介：韩鸿鹏（1980-），男，河南漯河人，博士研究生，副教授，现主要从事植物逆境生物学及分子生物学等研究工作。E-mail:hanhongpeng@163.com. 基金项目：河南省科技计划资助项目（122102110189，142102110123）；河南省973重大研究计划资助项目（2012CB114300）。