CHEN Xiaojie,GUO Xingke,ZHANG Yuan,et al.Study on Rapid Micropropagation Technique by Shoot Tip Culture of Ring Rot Resistant Apple Rootstock J35-8[J].Northern Horticulture,2015,39(13):107-110.[doi:10.11937/bfyy.201513031]
抗轮纹病苹果砧木J35-8茎尖培养快繁体系建立
- Title:
- Study on Rapid Micropropagation Technique by Shoot Tip Culture of Ring Rot Resistant Apple Rootstock J35-8
- 文章编号:
- 1001-0009（2015）13-0107-04
- Keywords:
- apple rootstock J35-8; shoot tip culture; tissue culture and rapid micropropagation; plant growth regulator; ring rot
- 分类号:
- S 661.103.6
- 文献标志码:
- A
- 摘要:
- 以抗轮纹病苹果砧木J35-8的茎尖为试材，通过筛选合适的培养基，研究了培养基中不同植物生长调节剂配比对芽苗诱导、增殖和生根的影响。结果表明：以MS为基本培养基，附加蔗糖35 g/L、琼脂6.2 g/L，适宜启动培养的植物生长调节剂配比为6-BA 1.00 mg/L+NAA 0.05 mg/L；适宜增殖培养的植物生长调节剂配比为6-BA 1.0 mg/L+NAA 0.050 mg/L，增殖系数可达到每4周8.50倍；以1/2MS为基本培养基，附加蔗糖15 g/L、琼脂6 g/L，适宜组培苗生根的植物生长调节剂配比为IAA 1.0 mg/L+IBA 0.3 mg/L，生根率达92.68%以上，每株苗平均生根9.35条，移栽成活率达100.00%。
- Abstract:
- Taking the shoot tips of the ring rot resistant apple rootstock J35-8 as materials,by selecting the optimal medium，the effects of plant growth regulators on tissue-cultured seedling induction，multiplication and rooting were researched.The results showed that the basic medium was MS supplemented with sugar 35 g/L and agar 6.2 g/L，the optimal plant growth regulators of start culture medium that suited multiplication were 6-BA 1.00 mg/L+NAA 0.05 mg/L.The optimal plant growth regulators in subculture medium were 6-BA 1.0 mg/L+NAA 0.050 mg/L，and the coefficient of multiplication were exceeded 8.50 times every four weeks.The optimal plant growth regulators for rooting were IAA 1.0 mg/L+IBA 0.3 mg/L，the rooting rates of shoots were 92.68%，the average number of rooting was 9.35.Then the rooted plantlets were transplanted，the survival rates were 100.00%.
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备注/Memo
第一作者简介：陈晓洁（1989-），女，河北张家口人，硕士研究生，研究方向为果树栽培生理。E-mail:15933593516@163.com.责任作者：徐继忠（1964-），男，河北唐山人，博士，教授，博士生导师，现主要从事果树生物技术及果树栽培生理与生态等研究工作。E-mail：xjzhxw@126.com.基金项目：河北省科技厅资助项目（14226307D-5）;农业部公益性行业科研专项资助项目（201203075-05）。