[1]鹿志伟,高建明,刘巧莲,等.芦笋组培快繁体系的建立[J].北方园艺,2015,39(09):83-86.[doi:10.11937/bfyy.201509024]
　LU Zhi-wei,GAO Jian-ming,LIU Qiao-lian,et al.The Establishment of Asparagus officinalis Tissue Culture and Rapid Propagation System[J].Northern Horticulture,2015,39(09):83-86.[doi:10.11937/bfyy.201509024]
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芦笋组培快繁体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年09 页码: 83-86 栏目: 出版日期: 2015-05-15
Title:
The Establishment of Asparagus officinalis Tissue Culture and Rapid Propagation System
文章编号:
1001-0009（2015）09-0083-04
作者:
鹿志伟1; 高建明1; 2 ; 刘巧莲2; 侯晓婉2; 易克贤2
（1.海南大学 农学院，海南 海口 570228；2.中国热带农业科学院 环境与植物保护研究所，海南 海口 571101)
Author(s):
LU Zhi-wei1; GAO Jian-ming1; 2 ; LIU Qiao-lian2; HOU Xiao-wan2; YI Ke-xian2
(1.College of Agronomy，Hainan University，Haikou，Hainan 570228；2.Institute of Environment and Plant Protection Institute，CATAS，Haikou，Hainan 571101)
关键词:
芦笋; 胚状体; 组培; 快繁
Keywords:
Asparagus officinalis; embryos; tissue culture; rapid propagation
分类号:
S 644.603.6
DOI:
10.11937/bfyy.201509024
文献标志码:
A
摘要:
以芦笋高产品种“井岗701”的无菌茎尖为试材，采用正交实验设计方法,研究嘧啶醇、KT、2,4-D、脱落酸、6-BA和NAA等不同植物激素浓度组合对愈伤组织、胚状体以及幼苗诱导的影响。结果表明：MS+2,4-D 2.0 mg/L+KT 1.0 mg/L诱导愈伤组织效果最好，出愈率达到80％左右。MS+0.70 mg/L嘧啶醇+0.10 mg/L NAA+0.50 mg/L KT诱导胚状体效果最好，诱导率高达95％。MS+NAA 0.5 mg/L+6-BA 1.0 mg/L诱导胚状体成苗率达到60％左右。室外移栽成活率高达90％，初步建立了芦笋由胚状体到幼苗的一步成苗体系。
Abstract:
The sterile shoot tips of high-yielding varieties of ‘Jinggang 701’ Asparagus officinals were used as experimental materials，using orthogonal design,the effect of different concentrations combinations of pyrimidinol，KT，2,4-D ，abscisic acid，6-BA and NAA on the inductions for callus，somatic embryoids and seedlings were studied.The results showed that the optimum inducing medium for callus was MS+2,4-D 2 mg/L+KT 1.0 mg/L,the inducing rate reached to 80％；the optimum inducing medium for embryos was MS+0.70 mg/L ancymidol+0.10 mg/L NAA+0.50 mg/L KT，the inducing rate reached to 95％；the optimum inducing medium for plantlet was MS+NAA 0.5 mg/L+6-BA 1.0 mg/L ，the inducing rate reached to 60％；the survival rate of outdoor transplant could be about 90％.The system of one-step inducing into plantlet for asparagus was initially established.

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备注/Memo

第一作者简介：鹿志伟（1990-），男，硕士研究生，研究方向为农业生物技术。E-mail：luzhiweihn12@yahoo.com.

责任作者：易克贤（1964-），男，博士，研究员，研究方向为作物遗传育种。E-mail：yikexian@126.com.
基金项目：国家公益性行业(农业)科研专项资助项目(201003074)。

更新日期/Last Update: 2015-08-01