[1]刘素慧,徐金强,刘庆涛,等.大蒜秸秆水浸液化感作用的研究[J].北方园艺,2015,39(09):10-13.[doi:10.11937/bfyy.201509003]
　LIU Su-hui,XU Jin-qiang,LIU Qing-tao,et al.Study of Allelopathy on Straw Aqueous Extracts of Garlic (Allium sativum L.)[J].Northern Horticulture,2015,39(09):10-13.[doi:10.11937/bfyy.201509003]
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大蒜秸秆水浸液化感作用的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年09 页码: 10-13 栏目: 出版日期: 2015-05-15
Title:
Study of Allelopathy on Straw Aqueous Extracts of Garlic (Allium sativum L.)
文章编号:
1001-0009(2015)09-0010-04
作者:
刘素慧; 徐金强; 刘庆涛; 尉辉
（山东农业工程学院，山东 济南 250100）
Author(s):
LIU Su-hui; XU Jin-qiang; LIU Qing-tao; WEI Hui
（Shandong Agricultural and Engineering University，Jinan，Shandong 250100）
关键词:
大蒜秸秆; 水浸液; 化感作用
Keywords:
garlic stalks; aqueous extracts; allelopathy
分类号:
S 633.4
DOI:
10.11937/bfyy.201509003
文献标志码:
A
摘要:
以大蒜秸秆水浸液为试材，以番茄、辣椒、黄瓜、莴苣、大葱和洋葱为受体材料，研究了大蒜秸秆水浸液对不同受体蔬菜的发芽率、胚芽和胚根长及鲜重的影响，用薄层层析试验，分离大蒜秸秆水浸液中的化感物质，研究其对莴苣的生物活性，并鉴定出抑制物质，为高效利用大蒜秸秆的化感作用提供理论依据。结果表明：不同浓度的大蒜秸秆水浸液对番茄和辣椒种子发芽率均有抑制作用，但对黄瓜种子的发芽率无明显影响；水浸液对番茄、黄瓜、辣椒胚芽和胚根的影响均为低浓度促进高浓度抑制的双重浓度效应；但对同属作物洋葱和大葱的萌芽指标均表现为抑制作用。经薄层层析得到的5个斑点中，斑点2、3、5对莴苣的萌芽有抑制作用，经GC-MS鉴定：斑点2中以芳香族化合物为主；斑点3主要以联苯和1-甲基萘等为主；斑点5以脂肪族酯类和芳香族化合物为主。
Abstract:
With different garlic stalks aqueous extracts as materials，and with tomatoes，peppers，cucumbers，lettuce，onions and welsh onions as receptor material，different receptors vegetable germination’ germ length and fresh weight，radicle length and fresh weight were studied by using different concentrations of aqueous extracts of garlic stalks.Afterward，allelochemicals of garlic stalks aqueous extracts were separated by thin layer chromatography test，and their biological activity on lettuce and then identified inhibitory substances were studied，in order to provide the theoretical basis for effectively using allelopathy of garlic stalks.The results showed that all concentration designed in this test restrained the germination rates of tomato and pepper，but had no obvious effect on cucumber’ germination rate；the influence of garlic straw aqueous extracts on the germination (embryo and radicle) of tomato，pepper and cucumber was dual concentration effect：the high concentration inhibited but the low concentrations displayed promoter action；garlic straw aqueous extracts had inhibition on the germination of onion and welsh onion.Five spots were observed on TLC plates under UV light，of these，spot 2，spot 3 and spot 5 suppressed lettuce emergence.The second spot contained diethyl phthalate and a derivative of phenol，biphenyl and 1-methyl-naphthalene were detected in the third spot.In the fifth spot，aromatic compounds and aliphatic ester were identified.

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备注/Memo

第一作者简介：刘素慧（1981-），女，山东菏泽人，博士，讲师，现主要从事蔬菜栽培生物学等研究工作。E-mail：liusuhui2003@163.com.

基金项目： 国家公益性行业（农业）科研专项经费资助项目（200903018）；山东省农业重大创新资助项目。

更新日期/Last Update: 2015-08-01