[1]郭臣臣,雷亚珍,刘春,等.豆瓣绿愈伤组织诱导及再生体系的建立[J].北方园艺,2015,39(08):117-120.[doi:10.11937/bfyy.201508031]
　GUO Chen-chen,LEI YA-zhen,LIU Chun,et al.Callus Induction and Establishment of the Regeneration System of Peperomia obtusifolia[J].Northern Horticulture,2015,39(08):117-120.[doi:10.11937/bfyy.201508031]
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豆瓣绿愈伤组织诱导及再生体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年08 页码: 117-120 栏目: 出版日期: 2015-04-30
Title:
Callus Induction and Establishment of the Regeneration System of Peperomia obtusifolia
文章编号:
1001-0009（2015）08-0117-04
作者:
郭臣臣1; 雷亚珍1; 刘春2; 张黎1
(1.宁夏大学 农学院，宁夏 银川 750021;2.中国农业科学院 蔬菜花卉研究所，北京 100081）
Author(s):
GUO Chen-chen1; LEI YA-zhen1; LIU Chun2; ZHANG Li1
(1.School of Agriculture，Ningxia University，Yinchuan，Ningxia 750021;2.Research Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081)
关键词:
豆瓣绿; 外植体; 再生体系; 愈伤组织; 诱导
Keywords:
Peperomia obtusifolia;  explant; regeneration system; calus; induction
分类号:
S 682.36
DOI:
10.11937/bfyy.201508031
文献标志码:
A
摘要:
以豆瓣绿的叶片、叶柄及带芽茎段为试材，筛选最佳外植体类型；使用激素6-BA与IBA，选出适宜诱导愈伤组织与芽增殖的培养基；研究活性炭对瓶苗生根的影响，选出适合的生根培养基。结果表明：带芽茎段是诱导愈伤组织的最佳外植体，褐化率仅为6.7%，而叶片和叶柄褐化严重，二者褐化率均超过60%；最佳诱导愈伤组织培养基为MS+6-BA 2.0 mg/L+IBA 0.1 mg/L，出愈率100.00%；最佳不定芽增殖培养基为MS+6-BA 0.5 mg/L+IBA 0.1 mg/L，增殖系数为13.8；最佳生根培养基为1/2MS+IBA 0.2 mg/L+活性炭1.00 g/L。
Abstract:
The leaves，petioles and stems with buds of Peperomia obtusifolia were chosen as the explants to select the best explant types，the combinations of 6-BA and IBA was used to select suitable medium of callus induction and bud multiplication；activated carbon was used to screen the appropriate rooting medium.The results showed that the stems with buds was suitable for inducing callus and the browning rate was only 6.7%，and the leaf and petiole browning rate were more than 60%.It indicated that the optimum medium for callus induction was MS+6-BA 2.0 mg/L+IBA 0.1 mg/L and the rate of callus was 100.00%;the best shoot proliferation was MS+6-BA 0.5 mg/L+IBA 0.1 mg/L and the multiplication coefficient was 13.8；the optimum root rooting medium was 1/2 MS+IBA 0.2 mg/L+AC 1.0 g/L.

参考文献/References:


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[4]王涛,彭立新.豆瓣绿(Peperomia obtusifolia)的组织培养与快速繁殖研究[J].天津农学院学报，2011(2):1-5.
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备注/Memo

第一作者简介：郭臣臣（1988-），男，硕士研究生，研究方向为观赏园林植物。

责任作者：张黎（1962-），女，硕士，教授，研究方向为园林植物与观赏园艺。E-mail：zhang_li9988@163.com.

更新日期/Last Update: 2015-07-24