WEI Mei-sheng,MA Jie,LI Gui-fen.Study on Detection of Grapevine virus A by Immunocapture Reverse Transcription-Polymerase Chain Reaction (IC-RT-PCR)[J].Northern Horticulture,2015,39(01):99-103.[doi:10.11937/bfyy.201501027]
葡萄A病毒的IC-RT-PCR检测研究
- Title:
- Study on Detection of Grapevine virus A by Immunocapture Reverse Transcription-Polymerase Chain Reaction (IC-RT-PCR)
- Keywords:
- Keywords: Grapevine virus A ; IC-RT-PCR
- 文献标志码:
- A
- 摘要:
- 葡萄A病毒是葡萄皱木综合证的病原之一，通常与其它病毒混合发生，造成葡萄产量的损失。为有效检测葡萄A病毒，该研究建立了免疫捕获-反转录-聚合酶链式反应检测方法（IC-RT-PCR），将免疫学和核酸扩增技术结合起来使用。结果表明：IC-RT-PCR方法检测的特异性较强，检测葡萄A病毒2个株系的结果为阳性，检测葡萄扇叶病毒、南芥菜花叶病毒、烟草环斑病毒结果为阴性。该方法检测提纯葡萄A病毒的灵敏度为10 ng/mL，检测葡萄A病毒感染的 Nicotiana benthamiana 叶片可稀释到10-6。
- Abstract:
- Abstract:Grapevine virus A (GVA) is one of pathogens causing grapevine rugose wood complex,which can be caused by many kinds of viruses simultaneously.In order to more effectively detecting this virus,an immunocapture reverse transcription-polymerase chain reaction （IC-RT-PCR) was developed for detecting Grapevine virus A in this study.The results showed that 2 strains of GVA were detected using this method.There was no cross reaction with Grapevine fanleaf virus , Arabis mosaic virus and Tobacco ringspot virus .The sensitivity of the assay was 10 ng/mL for detecting purified GVA.Extract from GVA infected Nicotiana benthamiana leaves was detected to a dilution of 10-6.
参考文献/References:
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备注/Memo
第一作者简介：魏梅生(1965-)，男,安徽巢湖人，硕士，研究员，现主要从事植物病毒病害的检疫检测等研究工作。E-mail：xsms2013@163.com. 基金项目：国家质量监督检验检疫总局课题资助项目（2012IK302）。