HUANG Min,LIANG Chun-hui,LI Xiu-ping,et al.Study on Optimization of Proliferation and Root Induction Technique of Anoectochilus roxburghii by Orthogonal Design[J].Northern Horticulture,2015,39(01):96-98.[doi:10.11937/bfyy.201501026]
应用正交设计优化金线莲继代增殖和生根培养的研究
- Title:
- Study on Optimization of Proliferation and Root Induction Technique of Anoectochilus roxburghii by Orthogonal Design
- 文献标志码:
- A
- 摘要:
- 摘 要：以金线莲试管苗为试材，应用L9(3 4)正交实验法研究不同因子对金线莲茎段继代增殖的影响及生根效果的影响，筛选出适宜于金线莲茎段增殖及生根的理想培养基。结果表明：试验因子对金线莲增殖系数的影响顺序为：6-BA＞蔗糖＞香蕉泥＞NAA，适合金线莲茎段增殖的培养基为：MS+2.5 mg/L 6-BA+0.25 mg/L NAA+75 g/L香蕉泥+28 g/L蔗糖，增殖系数为2.725；各因素对金线莲生根系数的影响程度大小依次为：基本培养基＞6-BA＞活性炭＞IBA，适合金线莲组培苗生根的培养基为1/2MS+0.25 mg/L 6-BA+5 mg/L IBA+2 g/L活性炭，平均生根数为2.405。
- Abstract:
- Abstract:Taking the test-tube seedling of Anoectochilus roxburghii as material,the optimization of culture medium for adventitious buds proliferation and rooting of Anoectochilus roxburghii were studied by orthogonal design L9(3 4).The results showed that,the effect for proliferation culture was in the order of 6-BA＞sucrose＞mashed bananas＞NAA,the optimizing medium for proliferation culture was MS+2.5 mg/L 6-BA+0.25 mg/L NAA+75 g/L mashed bananas+ 28 g/L sucrose with the multiplication coefficient of 2.725.The effect of the factor for root induction as follow：the basic culture medium＞6-BA＞activated carbon＞IBA,the optimizing medium for rooting culture was 1/2MS+0.25 mg/L 6-BA+5 mg/L IBA+2 g/L activated carbon and the number of roots per plantlets of 2.405.
参考文献/References:
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备注/Memo
第一作者简介：黄敏（1987-），女，硕士，助理实验师，现主要从事植物组织培养等研究工作。E-mail：huangmin0102@163.com.基金项目：亚热带农业生物资源保护与利用国家重点实验室开放课题资助项目（SKL-CUSAb-2013-08）；国家自然科学基金资助项目（31371642）；广东农工商职业技术学院科研重点资助项目（xyzd1308）。