YANG Shen-ming,WANG Zhen-ji,YANG Hong-wei,et al.Study on Extraction Process of Total Flavonoid in Ficus tikoua[J].Northern Horticulture,2014,38(24):126-130.
地石榴中总黄酮提取工艺研究
- Title:
- Study on Extraction Process of Total Flavonoid in Ficus tikoua
- 文章编号:
- 1001-0009（2014）24-0126-05
- Keywords:
- Ficus tikoua Bur.; orthogonal test; total flavonoids
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以地石榴为试材，采用乙醇回流法从地石榴雌雄植株中提取总黄酮，通过单因素试验和正交实验，以地石榴总黄酮提取率为指示，考察了乙醇体积分数、料液比、提取温度、提取时间对提取率的影响，优化了地石榴总黄酮的提取工艺。结果表明:最佳提取工艺参数为乙醇体积分数65%，料液比1∶25（g/mL），提取温度80℃，提取时间120 min。在此条件下，地石榴雄、雌植株中总黄酮的提取率分别为1.167%和1.147%，加标平均回收率为98.56%，RSD值为0.84%。该方法操作简单，准确度较高，精密度好，可作为地石榴中总黄酮的提取和含量测定。
- Abstract:
- Taking Ficus tikoua Bur.as material，the total flavonoids in Ficus ticoua was extracted by means of ethanol refluxing method.The extraction technology of total flavonoids from Ficus ticoua was optimized through single factor experiment and orthogonal experiment.With the extraction rate of total flavonoids in Ficus ticoua as the indicator，effect of the volume fraction of ethanol，solid-liquid ratio，extraction temperature，extraction time on the extraction rate were studied.The results showed that the optimum extraction process parameters were followed,the volume fraction of ethanol 65%，ratio of solid to liquid 1∶25 (g∶mL)，extraction time 120 minutes，and extraction temperature 80℃.The average extraction rate of total flavonoids in male and female trees were 1.167% and 1.147% respectively，the average recovery rate was 98.56% under such condition.RSD value was 0.84%.The method has the advantages of simple operation，high accuracy，good precision，could be used as the extraction and content of total flavonoids in Ficus tikoua determination.
参考文献/References:
[1]张秀实,吴征镒.中国植物志:被子植物门.双子叶植物纲.桑科[M].23卷,第1分册.北京:科学出版社,1997：156-157. [2]云南省药物研究所.云南天然药物图鉴[M].第3卷.昆明:云南科技出版社,2007:165. [3]陈业高.植物化学成分[M].北京:化学工业出版社,2004:229. [4]徐向容,王文华,李华斌.荧光法测定Fenton反应产生的羟自由基[J].分析化学,1998,26(12):1460-1463. [5]陈丛瑾,黄克瀛,王贵武,等.香椿叶总黄酮不同提取方法的比较[J].食品研究与开发,2008,29(3):57-60. [6]罗伯特D.布朗.最新仪器分析技术全书[M].北京:化学工业出版社,1987:181. [7]何兰,姜志宏.天然产物资源化学[M].北京:科学出版社,2008:356. [8]彭俊文,朱晓伟,陈建平,等.黄花蒿中黄酮类成分的分离鉴定[J].内蒙古医学院学报，2011,33(2):152-154. [9]夏道宗,于新芬,马志杰.二次回归通用旋转设计优化土茯苓总黄酮的提取工艺[J].中华中医药学刊,2009,27(7):1460-1463. [10]张春兰,张锐利,张建花,等.枣核中黄酮类化合物的提取工艺[J].食品研究与开发,2011,32(9):33-35. [11]贺波,李小定,彭定祥,等.苎麻叶中黄酮类化合物的提取工艺研究[J].食品工业科技,2010,31(10):259-262. [12]古丽娜尔·夏衣马尔旦.黄连中总黄酮含量测定及提取工艺研究[J].新疆师范大学学报,2010,29(1):75-78.
相似文献/References:
[1]周金梅,赵文若,建德锋,等.不同品种和栽植密度及整枝方式对黄秋葵产量的影响[J].北方园艺,2013,37(04):34.
[2]刘晓莉,田海娟,钟容,等.盐沉淀法提取紫苏活性蛋白的工艺研究[J].北方园艺,2013,37(04):147.
[3]史艳财,邹 蓉,韦记青,等.黄花倒水莲总黄酮提取工艺研究[J].北方园艺,2014,38(12):134.
SHI Yan-cai,ZOU Rong,WEI Ji-qing,et al. Study on Extraction Condition of Flavonoid in Polygala fallax[J].Northern Horticulture,2014,38(24):134.
[4]叶 林,李春江,张光弟,等.不同育苗穴数、基质配比和施肥量对温室辣椒幼苗生长及其秧苗质量的影响[J].北方园艺,2014,38(13):50.
YE Lin,LI Chun-jiang,ZHANG Guang-di,et al.Effects of Different Seedling Number of Holes，Substrates and Fertilization on the Growth and Quality of Greenhouse Pepper Seedlings[J].Northern Horticulture,2014,38(24):50.
[5]张 颖.青蒿素的提取工艺优化研究[J].北方园艺,2014,38(11):122.
ZHANG Ying.Study on Optimization of the Extraction of Artemisinin[J].Northern Horticulture,2014,38(24):122.
[6]杨申明,王振吉,沈继森.彝药猪鬃草中总黄酮提取工艺研究[J].北方园艺,2014,38(11):125.
YANG Shen-ming,WANG Zhen-ji,SHEN Ji-sen.Study of the Extraction Technology of Total Flavonoids in Adiantum capillus-veneris[J].Northern Horticulture,2014,38(24):125.
[7]王 嵩,马 杰,赵 维,等.黔西北景天三七高产栽培优化研究[J].北方园艺,2014,38(11):147.
WANG Song,MA Jie,ZHAO Wei,et al.Research on Optimazation Growing Cutivation of Sedum aizoon L. in Northwest Guizhou[J].Northern Horticulture,2014,38(24):147.
[8]王亚红,王亚丽,祝 波.瓦松总黄酮的索氏提取工艺研究[J].北方园艺,2014,38(10):130.
WANG Ya-hong,WANG Ya-li,ZHU Bo.Study on Soxhlet Extraction Technology of Total Flavonoids From Orostachys fimbriatus[J].Northern Horticulture,2014,38(24):130.
[9]杨艳俊,王一洋.微波辅助法提取景天三七中齐墩果酸的工艺研究[J].北方园艺,2014,38(10):152.
YANG Yan-jun,WANG Yi-yang.Study on Extraction Technology of Oleanolic Acid From Sedum aizoon L.[J].Northern Horticulture,2014,38(24):152.
[10]姜 喜,陈加利,肖 巍,等.扁桃茎段增殖培养基的筛选[J].北方园艺,2013,37(22):111.
JIANG Xi,CHEN Jia-li,XIAO Wei,et al.Screening of the Multiplying Medium of Almond Stem[J].Northern Horticulture,2013,37(24):111.
备注/Memo
第一作者简介：杨申明（1976-），男，本科，实验师，现主要从事天然有机产物化学等研究工作。E-mail：ysm@cxtc.edu.cn.