JIANG Xi,CHEN Jia-li,XIAO Wei,et al.Screening of the Multiplying Medium of Almond Stem[J].Northern Horticulture,2013,37(22):111-113.
扁桃茎段增殖培养基的筛选
- Title:
- Screening of the Multiplying Medium of Almond Stem
- 文章编号:
- 1001-0009(2013)22-0111-03
- Keywords:
- almond stem; multiplying medium; orthogonal experiment
- 分类号:
- S 662.9
- 文献标志码:
- A
- 摘要:
- 以“莎车14号”、“莎车大3号”2个扁桃品种的茎段为试材，采用L9（34）正交实验设计，研究了适合“莎车14号”、“莎车大3号”茎段增殖的培养基。结果表明：外植体灭菌以70%乙醇消毒8 s+2%和4%次氯酸钠消毒10 min效果良好，茎段平均成活率为95%。扁桃“莎车大3号”的最优增殖培养基配比为MS+6-BA 1.0 mg/L+IAA 0.1 mg/L，增殖系数4.63；扁桃“莎车14号”的最优增殖培养基配比为MS+6-BA 1.0 mg/L+IAA 0.2 mg/L+GA3 0.1 mg/L，增殖系数4.20。
- Abstract:
- Selecting almond cultivars‘Shacheda3’,’Shache14’stem as materials,the ideal multiplying medium of them were screened through the orthogonal test of L9(34).The results showed that stems disinfection effect treated by 2%~4% NaClO for 10 min,after 70% ethanol for 8 seconds was the best,and the average survival rate could reach 95%.The optimum medium of multiplication for ‘Shacheda3’ was MS+6-BA 1.0 mg/L+IAA 0.1 mg/L,and the multiplication rate was 4.63;the optimum medium of multiplication for‘Shache 14’was MS+6-BA 1.0 mg/L+IAA 0.2 mg/L+GA3 0.1 mg/L,and the multiplication rate was 4.20.
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相似文献/References:
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备注/Memo
第一作者简介：姜喜（1981-），女，硕士，实验师，研究方向为果树栽培生理。E-mail:837861277@qq.com.
责任作者：王琳（1969-），女，硕士，副教授，现主要从事林学和园林教学与科研等工作。E-mail:1531956730@qq.com.
基金项目：塔里木大学校长基金资助项目（TDZKSSZD201202）；
国家级大学生创新创业训练计划资助项目（TDGCX201214）；
塔里木大学校长基金资助项目（TDZKSS201216）。
收稿日期：2013-06-17