ZHAO Yu-hui,WANG Gang,SU Kai,et al.Genetic Diversity of Total Flavonoids in Leaves of Hawthorn (Crataegus spp.)[J].Northern Horticulture,2014,38(24):88-92.
山楂属(Crataegus spp.)种质资源叶片总黄酮遗传多样性分析
- Title:
- Genetic Diversity of Total Flavonoids in Leaves of Hawthorn (Crataegus spp.)
- 文章编号:
- 1001-0009(2014)24-0088-05
- Keywords:
- hawthorn leaves; total flavonoid content; genetic diversity
- 分类号:
- S 661.5
- 文献标志码:
- A
- 摘要:
- 以国家果树种质沈阳山楂资源圃收集的8个种135份山楂种质资源为试材,采用NaNO2-Al(NO3)3-NaOH 比色法,研究山楂属资源总黄酮含量及遗传多样性水平。结果表明:山楂属资源黄酮含量具丰富的变异,变异系数为57.07%,其变幅为0.25%~11.65%,通过山楂属植物叶黄酮类成分比较研究,发现了一些有利用价值的叶用资源。
- Abstract:
- Taking 135 germplasm resources which belong to 8 kinds species come from National Hawthorn Germplasm Nursery of Shenyang as materials,using NaNO2-Al(NO3)3-NaOH colorimetery to research the total flavonoids and the genetic diversity of hawthorn.The results showed that the flavonoid content of hawthorn possesses abundant variation,and the variable coefficient was 57.07%,the variatble amplitude was 0.25%-11.65%.It was found that some valuble leaf resources through comparative study for the flavonoids in hawthorn.
参考文献/References:
[1]
赵焕谆
,
丰宝田
.
中国果树志·山楂卷
[M].
北京
:
中国林业出版社
,1996:14-26.
[2]谢如玉,戴伦凯,郭梦如,等.山里红的成分分析及国产山楂属植物果实的比较[J].植物学报,1981,23(5):283-287. [3]沈燕琳,董文轩,李鲜,等.山楂酚类物质及其生物活性研究进展[J].园艺学报,2013,40(9):1691-1700. [4]鲁巍巍.中国山楂属(Crataegusspp.)植物叶黄酮多样性及其代谢生理研究[D].沈阳:沈阳农业大学,2008. [5]王敏,万丽,周立,等.北山楂叶与云南山楂叶中总黄酮与金丝桃苷含量的比较[J].现代生物医学进展,2008(8):694-695. [6]孙立立,谢鸿霞,孙敬勇,等.比色法测定山楂中总黄酮的含量[J].中成药,2001(23):748-750. [7]肖志红,刘红彦,韩松,等.不同山楂品种总黄酮含量的检测[J].河南农业科学,2006(6):101-102. [8]石瑞平,周庆英,陈兴菊.不同生长期山楂叶中黄酮类有效成分的含量测定[J].药品检测,2002(11):55. [9]王光全,孟庆杰,扈学立,等.山楂种质器官总黄酮含量的测定分析及其资源利用研究[J].食品科学,2005(26):307-309. [10]孟庆杰,王光全.山楂种质果实营养成分分析及其资源利用研究[J].河北农业大学学报,2005,28(1):21-23. [11]李化,杨滨.RP-HPLC法测定山楂中黄酮类成分的含量[J].药物分析杂志,2006,26(7):881-884. [12]马燕斌,曾建勋,张俊鹏,等.紫外-可见分光光度法测定不同产地山楂果实中总黄酮含量[J].中国药物经济学,2013(6):24-26. [13]许正斌,佟婉筠,孟庆生,等.山楂叶总黄酮含量变化[J].自然资源研究,1987(3):55-58. [14]Cheng S Y,Wang Y,Fei Y J,et al.Studies on the effects of different treatments on flavonoids contents in Ginkgo bilobaleaves and their regulating mechanism[J].Journal of Fruit Science,2004,21(2):116-119. [15]Sabina H,Aliya R.Seaweed as a new source of flavone,scutellarein 4′-methyl ether[J].Pak J Bot,2009,41(4):1927-1930. [16]Kaya B,Menemen Y,Saltan F Z.Flavonoids in the endemic species of AlchemillaL.,(section AlchemillaL.subsection calycanthum rothm.ser.elatae rothm.)from north-east black sea region in turkey[J].Pak J Bot,2012,44(2):595-597. [17]Jahan N,Rahman K U,Ali S,et al.Phenolic acid and flavonoid contents of gemmo-modified and native extracts of some indigenous medicinal plants[J].Pak J Bot,2013,45(5):1515-1519.
相似文献/References:
[1]张 虹,王景李,白红丽,等.云南翠雀花根中总黄酮提取方法的研究[J].北方园艺,2014,38(13):153.
ZHANG Hong,WANG Jing-li,BAI Hong-li,et al.Study on the Extraction Method Total Flavonoids in Root of the Delphinium yunnanense[J].Northern Horticulture,2014,38(24):153.
[2]杨申明,王振吉,沈继森.彝药猪鬃草中总黄酮提取工艺研究[J].北方园艺,2014,38(11):125.
YANG Shen-ming,WANG Zhen-ji,SHEN Ji-sen.Study of the Extraction Technology of Total Flavonoids in Adiantum capillus-veneris[J].Northern Horticulture,2014,38(24):125.
[3]王亚红,王亚丽,祝 波.瓦松总黄酮的索氏提取工艺研究[J].北方园艺,2014,38(10):130.
WANG Ya-hong,WANG Ya-li,ZHU Bo.Study on Soxhlet Extraction Technology of Total Flavonoids From Orostachys fimbriatus[J].Northern Horticulture,2014,38(24):130.
[4]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(24):154.
[5]闵莉静,李敬芬,赵明星.超声辅助提取橘皮总黄酮的工艺优化研究[J].北方园艺,2013,37(13):172.
MIN Li-jing,LI Jing-fen,ZHAO Ming-xing.Study on Optimization of Ultrasonic Assisted Extraction Technology of ?Total Flavonoids from Orange Peel[J].Northern Horticulture,2013,37(24):172.
[6]韩凤波,陈静雅.不同采收期对香椿总黄酮和总皂苷含量的影响[J].北方园艺,2014,38(02):159.
HAN Feng-bo,CHEN Jing-ya.Effect of Harvest Season on Total Saponins Content and Total Flavone Content of Toona sinensis [J].Northern Horticulture,2014,38(24):159.
[7]任吉君,王艳,肖华凤.香薷总黄酮萃取工艺参数优化研究[J].北方园艺,2013,37(14):140.
REN Ji-jun,WANG Yan,XIAO Hua-feng.Study on Optimized Extraction of Total Flavones from Elsholtzia ciliata Thunb.[J].Northern Horticulture,2013,37(24):140.
[8]王晓洁,盛东峰.不同溶剂对女贞子黄酮提取效果和抑菌活性的影响[J].北方园艺,2014,38(09):140.
WANG Xiao-jie,SHENG Dong-feng.Effect of Different Solvents on Extraction of Effective Total Flavones and Antibacterial Activity of Ligustrum lucidum [J].Northern Horticulture,2014,38(24):140.
[9]杨艳俊,王亚红,王君龙.景天三七总黄酮大孔吸附树脂纯化工艺研究[J].北方园艺,2014,38(07):144.
YANG Yan-jun,WANG Ya-hong,WANG Jun-long.Study on the Purification Process of Total Flavonoids of Sedum aizoon L. by Macroporous Adsorption Resin[J].Northern Horticulture,2014,38(24):144.
[10]朱金霞,郑国保,孔德杰,等.甘草中甘草苷、甘草酸及总黄酮的测定及其含量相关性研究[J].北方园艺,2014,38(03):149.
ZHU Jin-xia,ZHENG Guo-bao,KONG De-jie,et al.Study on the Determination of Liquiritin,Glycyrrhizic Acid and Total Flavonoids in Licoric and Their Relationships[J].Northern Horticulture,2014,38(24):149.
备注/Memo
第一作者简介:赵玉辉(1976-),女,博士,副研究员,现主要从事果树种质资源鉴定与评价等研究工作。E-mail:zhaoyuhui76@126.com.