MA Ling-ling,WANG Peng,WANG Shu-an,et al.Effect of Sampling Time and Hormones on Axillary Buds Induction of ‘Yuqiu No.1’[J].Northern Horticulture,2014,38(13):84-87.
取材时间和激素对“豫楸1号”腋芽诱导的影响
- Title:
- Effect of Sampling Time and Hormones on Axillary Buds Induction of ‘Yuqiu No.1’
- 文章编号:
- 1001-0009（2014）13-0084-04
- Keywords:
- ‘Yuqiu No.1’; axillary bud induction; hormones; browning
- 分类号:
- S 687
- 文献标志码:
- A
- 摘要:
- 以“豫楸1号”为试材，研究了灭菌方法、基本培养基类型、激素浓度和取材时间对“豫楸1号”茎段腋芽诱导的影响。结果表明：不同基本培养基对“豫楸1号”腋芽诱导的影响差异显著，MS为基本培养基诱导腋芽的诱导率最高；多菌灵浊液浸泡60 min、酒精灭菌30 s、升汞浸泡9 min的灭菌方法最适合“豫楸1号”诱导腋芽萌发；“豫楸1号”最适的腋芽诱导培养基为MS+0.8 mg/L 6-BA+0.10 mg/L IBA；建立“豫楸1号”组培快繁体系最佳取材时间是4月，染菌率、褐化率低，腋芽诱导率高，腋芽萌发、生长快。
- Abstract:
- Taking ‘Yuqiu No.1’ as material,the effect of methods of sterilization，types of basic culture medium，plant growth regulators and sampling time on axillary bud induction was studied.The results showed that significant difference was caused by using different culture mediums in which MS medium induced the most axillarys.The best axillary buds induction profile were explants soaked in carbendazim turbid liquid for 60 min，alcohol sterilization 30 s，soaked in mercuric chloride 9 min.The most suitable medium to induce axillary buds from ‘Yuqiu No.1’ was MS+0.8 mg/L 6-BA+0.10 mg/L IBA.The optimal sampling time to establish ‘Yuqiu No.1’ tissue culture system was in April，the contamination rate and browning rate were low，bud induction rate was high，axillary buds germinated and grew fast.
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备注/Memo
第一作者简介：马玲玲（1990-），女，河南鹤壁人，硕士研究生，研究方向为园林植物。E-mail：mllbeautiful@126.com.