WANG Fei,CHENG Lu.Study on Cluster Shoots Induction From the Stems of Clematis fusca[J].Northern Horticulture,2014,38(07):86-90.
褐毛铁线莲茎段诱导丛生芽的研究
- Title:
- Study on Cluster Shoots Induction From the Stems of Clematis fusca
- 文章编号:
- 1001-0009（2014）07-0086-05
- Keywords:
- Clematis fusca; stems with buds; adventitious buds; tissue culture
- 分类号:
- S 685.99
- 文献标志码:
- A
- 摘要:
- 以褐毛铁线莲(Clematis fusca)带芽茎段为外植体进行组织培养，研究了不同取材季节及植物生长调节剂对茎段诱导不定芽的影响。结果表明：春季为外植体取材的最佳季节，带芽茎段初代培养的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.05 mg/L，不定芽萌发率为93.33%，带芽茎段的继代增殖培养的最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+KT 1.0 mg/L，不定芽的增殖系数为2.63。
- Abstract:
- Taking Clematis fusca ?with bud stem segments as explants for tissue culture,the effect of differernt seasons and plant growth regulator on the adventitious buds induction from the stems were studied.The results showed that the best season to collect the explain was in spring.The best initial medium from stems with buds was MS+6-BA 1.0 mg/L+NAA 0.05 mg/L,the germination rate of adventitious buds was 93.33%,the best culture medium of proliferation from stems with buds was MS+6-BA 2.0 mg/L+NAA 0.05 mg/L+KT 1.0 mg/L,the proliferation index of adventitious buds was 2.63.
参考文献/References:
[1]王文采,李良千.铁线莲属一新分类系统[J].植物分类学报,2005,43(5):431-488. [2]严雄梁,吴璐璐,季梦成.中国铁线莲属植物研究现状及展望[J].江西科学,2008,26(5):832-837. [3]李新伟,李建强,王映明,等.湖北铁线莲属的区系特征及地理分布[J].武汉植物学研究,2004,22(4)：294-300. [4]Chaudhary M I,Qing H,Xiao P G，et al.Clematis huchouensis.Tamura:a traditional Chinese herbal medicine and its qualitycontrol using a high performance liquid chromatography technique[J].Biological Pharmaceutical Bulletin,2007,30(1):165-168. [5]Peng H,Lv H,Wang Y,et al.Clematis montana lectin，a novel mannose-binding lectin from traditional Chinese medicine with antiviral and apoptosis-inducing activities[J].Peptides,2009,30(10)：1805-1815. [6]Afolayan A J,Yakubu M T,Appidi J R,et al.Toxicological implications of aqueous extract of Clematis brachiata?Thunb.leaves in male Wistar rats[J].African Journal of Pharmacy and Pharmacology,2009,3(11):531-538． [7]普春霞,杨竹雅,刘小莉.云南铁线莲属12种药用植物的RAPD 分析[J].云南中医学院学报,2008，31(5)：37-41. [8]王文采.中国植物志[M].北京：科学出版社,1980. [9]张启香.观赏型铁线莲的引种及生物学研究[D].南京：南京林业大学,2007. [10]袁迎燕,石大兴,王米力,等.毛蕊铁线莲组织培养与植株再生研究[J].植物生理学通讯,2009,45(9):895. [11]王辉.三种中国野生铁线莲组织培养研究[D].南京：南京林业大学,2012. [12]吴荣,林萍,樊国盛,等.铁线莲‘Gipsy Queen’组织培养与快速繁殖(简报)[J].亚热带植物科学,2011,40(2):68-69. [13]谷延泽,高瑞彦.植物组织培养中的褐化现象及防治措施[J].河北农业科学,2008,12(6):56-58. [14]袁迎燕.毛蕊铁线莲组织培养与植株再生研究[D].雅安：四川农业大学,2010. [15]潘娟,李先源,李名杨.植物组织培养过程中常见问题及解决方法[J].安徽农业科学,2009,37（6）:2392-2394. [16]杨丽琴,李瑞,王俊,等.植物组织培养的三大难题[J].北方园艺,2008(4):104-107. [17]郭艳,杨海玲.植物组织培养中的褐化现象及解决途径[J].山西农业科学,2009,37(7):14-16. [18]祁宏英,徐洪国,张志.矮牵牛组织培养及不同基质对生根的影响[J].北方园艺,2012(15):137-139. [19]孔萌萌,周根余.芦笋组织培养生根技术[J].上海师范大学学报(自然科学版),2006(35):93-94.
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备注/Memo
第一作者简介：王非（1975-），女，吉林长春人，博士，副教授，研究方向为园林植物种质资源。E-mail:shuijing7539@163.com.