PENG Hai-jun,HU Run-huai.Optimization Conditions of Tissue Culture of Crocus sativus L.Using Orthogonal Experiment[J].Northern Horticulture,2014,38(06):111-114.
正交优化西红花组培快繁优化
- Title:
- Optimization Conditions of Tissue Culture of Crocus sativus L.Using Orthogonal Experiment
- 文章编号:
- 1001-0009(2014)06-0111-04
- Keywords:
- Crocus sativus L.; orthogonal experiment; induction rate; tissue culture; rapid propagation
- 分类号:
- R 282.71
- 文献标志码:
- A
- 摘要:
- 以西红花带芽的球茎切块为外植体，采用正交实验，通过极差分析、方差分析和多重比较，优选愈伤组织诱导条件、丛生芽增殖条件和球茎诱导条件。结果表明：适宜愈伤组织诱导培养基为1/2MS+NAA 2.0 mg/L+6-BA 0.4 mg/L；丛生芽增殖培养基为1/2MS+NAA 0.6 mg/L+6-BA 2.0 mg/L；球茎诱导培养基为1/2MS+NAA 0.5 mg/L+10%香蕉汁。
- Abstract:
- Using corm of Crocus sativus L. as explant,culture medium for the induction of callus,adventitious bud proliferation and the induction of corm were optimized by orthogonal design,through the method of factor index analysis,variance analysis and multiple comparison.The results showed that 1/2MS+NAA 2.0 mg/L+6-BA 0.4 mg/L was the best medium for induction of callus,the appropriate medium for adventitious buds proliferation was 1/2MS medium with NAA 0.6 mg/L and 6-BA 2.0 mg/L,1/2MS+0.5 mg/L NAA+10% banana juice was suitable for corm induction of saffron.
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备注/Memo
第一作者简介：彭海君（1988-），男，浙江临安人，硕士研究生，研究方向为药用植物资源利用。E-mail:penghaijun8881@163.com.