ZHANG Yan-zhen,ZHOU Hui-ming,WEI Sheng-long,et al.Optimization Test of Four Inorganic Salts on the Mycelia Growth of Lyophyllum decastes[J].Northern Horticulture,2014,38(05):135-138.
四种无机盐对荷叶离褶伞菌丝生长的优化试验
- Title:
- Optimization Test of Four Inorganic Salts on the Mycelia Growth of Lyophyllum decastes
- 文章编号:
- 1001-0009(2014)05-0135-04
- 分类号:
- S 646.19
- 文献标志码:
- A
- 摘要:
- 以荷叶离褶伞菌株为试材，采用L9（34）正交实验设计，在PDA基础培养基上测定了4种无机盐及其正交组合对其菌丝生长的影响，以期为其优质栽培提供理论依据。结果表明：荷叶离褶伞菌丝萌发与生长的最适组合为ZnSO4 2.0 g/L、MgSO4 4.0 g/L、KH2PO4 4.0 g/L、FeSO4 1.5 g/L；高浓度的ZnSO4、MgSO4、KH2PO4以及FeSO4对菌丝生长有抑制作用，其中MgSO4浓度≥10.0 g/L时，菌丝停止生长；4种无机盐协同作用对荷叶离褶伞菌丝生长影响的大小依次为FeSO4>MgSO4>KH2PO4>ZnSO4；各组合之间的菌丝生长速度差异极显著，不同组合间有明显的交互作用，最佳组合为2.0 g/L ZnSO4+6.0 g/L MgSO4+6.0 g/L KH2PO4+1.0 g/L FeSO4。
- Abstract:
- Taking Lyophyllum decastes as material,effects of four inorganic salts and their orthogonal combination on the mycelial growth of Lyophyllum decastes were investigated by L9(34) orthogonal design on the PDA culture medium in this study，it could provide the theoretical basis of its high quality cultivation.The results showed that the best concentration on optical mycelia germination and growth of Lyophyllum decastes was ZnSO4 2.0 g/L，MgSO4 4.0 g/L，KH2PO4 4.0 g/L，FeSO4 1.5 g/L.ZnSO4，MgSO4，KH2PO4 and FeSO4 at high concentration could inhibit the mycelia growth，and mycelium could stop growth at a concentration of MgSO4≥10 g/L.The cooperative effects of four inorganic salts on the mycelial growth showed the impact order was FeSO4>MgSO4>KH2PO4>ZnSO4.Mycelial growth rate revealed that each combination had a highly significant difference and significant interaction.The best combination for the mycelia growth of?Lyophyllum decastes was 2.0 g/L ZnSO4+6.0 g/L MgSO4+6.0 g/L KH2PO4+1.0 g/L FeSO4.
参考文献/References:
[1]席亚丽,王治江,王晓琴,等.荷叶离褶伞子实体、菌丝体和发酵液营养成分比较分析[J].食品科学,2010,31(6)：155-157. [2]Pokhrel C P,Ohga S.Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes[J].Food Chemistry,2007,105(2)：641-646. [3]Miura T,Kubo M,Itoh Y,et al.Antidiabetic activity of Lyophyllum decastes in genetically type 2 diabetic mice[J].Biological and Pharmaceutical Bulletin,2002,25(9)：1234-1237. [4]Ukawa Y,Furuichi Y,Kokean Y,et al.Effect of Hatakeshimeji (Lyophyllum decastes?Sing.) Mushroom on serum lipid levels in rats[J].Journal of Nutritional Science and Vitaminology,2002,48(1)：73-76. [5]王守现,刘宇,许峰,等.荷叶离褶伞菌株的ITS鉴定及生物学特性研究[J].中国农学通报,2012,28(28)：148-152. [6]魏生龙,于海萍,连海丽,等.矿质营养与其他生长物质对荷叶离褶伞菌丝生长的影响[J].菌物学报,2008,27(2)：201-208. [7]张春梅,宋海,魏生龙.荷叶离褶伞菌丝体多糖的提取及还原力的研究[J].中国食用菌,2012,31(6)：44-48. [8]张汉燚,张芬琴,王小明,等.荷叶离褶伞子实体营养成分分析与评价[J].菌物学报,2008,27(5)：696-700. [9]张凤琴,李彩霞,李鹏,等.荷叶离褶伞可溶性多糖提取工艺研究[J].食品工业科技,2010(5)：224-225. [10]汪小庆,周德群,张小雷,等.离褶伞属分类学研究概况[J].中国食用菌,2013,32(1)：1-3. [11]李晓,李玉.中国离褶伞属真菌研究进展[J].食用菌学报,2009,16(3)：75-79. [12]Nakamura T,Itokawa Y,Tajima M,et al.Radioprotective effect of Lyophyllum decastes?and the effect on immunological functions in irradiated mice[J].Journal of Traditional Chinese Medicine,2007,27(1)：70-75. [13]Ukawa Y,Izumi Y,Ohbuchi T,et al.Oral administration of the extract from Hatakeshimeji?(Lyophyllum decastes?Sing.) mushroom inhibits the development of atopic dermatitis-like skin lesions in NC/Nga mice[J].Journal of Nutritional Science and Vitaminology,2007,53(3)：293-296. [14]Rodriguez Estrada A E,Royse D J.Yield，size and bacterial blotch resistance of Pleurotus eryngii?grown on cottonseed hulls/oak sawdust supplemented with manganese，copper and whole ground soybean[J].Bioresource Technology,2007,98(10)：1898-1906. [15]吴郑武.白灵菇栽培配方、Cu2+及Mn2+对其生长发育影响研究[D].长沙：湖南农业大学，2012. [16]Igamberdiev A U,Kleczkowski L A.Implications of adenylate kinase-governed equilibrium of adenylates on contents of free magnesium in plant cells and compartments[J].Biochemical Journal,2001,360(1)：225-2311. [17]Igamberdiev A U,Kleczkowski L A.Membrane potential,adenylate levels and Mg2+ are interconnected via adenylate kinase equilibrium in plant cells[J].Biochimica et Biophysica Acta (BBA)-Bioenergetics,2003,1607(2)：111-119. [18]杨琴,杜双田,张桂香.大肥蘑菇营养生理研究[J].食用菌学报,2012,19(3)：63-68. [19]谢天娇.杏鲍菇液体培养微量元素对其菌丝生物量的影响[J].安徽农业科学,2012,40(21)：10757-10758. [20]吴秀珍,王秋玲,杜双田,等.无机盐、维生素及生长调节剂对斑玉蕈菌丝生长的影响[J].西北农林科技大学学报（自然科学版）,2012,40(3)：158-162. [21]李盛旻,王永宏,王广耀.不同矿质元素对褐口蘑菌丝体生长的影响[J].北方园艺,2012(14)：166-167.
相似文献/References:
[1]周会明,张焱珍,席亚丽,等.五株荷叶离褶伞碳氮源及适合发酵菌株的筛选[J].北方园艺,2014,38(09):159.
ZHOU Hui-ming,ZHANG Yan-zhen,XI Ya-li,et al.Screening of Different Carbon and Nitrogen Sources and Suitable for Fermentation on Five Strains of Lyophyllum decastes[J].Northern Horticulture,2014,38(05):159.
[2]周会明,张焱珍,陈银辉,等.荷叶离褶伞液体发酵菌株筛选研究[J].北方园艺,2014,38(03):134.
ZHOU Hui-ming,ZHANG Yan-zhen,CHEN Yin-hui,et al.Study on Screening of Liquid Fermentation Strains of Lyophyllum decastes[J].Northern Horticulture,2014,38(05):134.
[3]简利茹,杜双田.氮源和无机盐对蛹虫草子座产量及虫草素和腺苷的影响[J].北方园艺,2016,40(19):163.[doi:10.11937/bfyy.201619041]
JIAN Liru,DU Shuangtian.Effect of Nitrogen and Inorganic Salt on Production of Stroma，Cordycepin and Adenosine in Cordyceps militaris[J].Northern Horticulture,2016,40(05):163.[doi:10.11937/bfyy.201619041]
[4]姜慧,林辰壹,高攀,等.五种无机盐对大球盖菇菌丝生长的影响[J].北方园艺,2020,44(14):128.[doi:10.11937/bfyy.20194950]
JIANG Hui,LIN Chenyi,GAO Pan,et al.Effects of Five Inorganic Salts on the Mycelial Growth of Stropharia rugoso-annulata[J].Northern Horticulture,2020,44(05):128.[doi:10.11937/bfyy.20194950]
[5]刘娜,张敏,张鹏,等.野生荷叶离褶伞菌株的鉴定及其生物学特性研究[J].北方园艺,2023,(09):119.[doi:10.11937/bfyy.20223501]
LIU Na,ZHANG Min,ZHANG Peng,et al.Study on Identification and Biological Characteristics of Wild Lyophyllum decastes[J].Northern Horticulture,2023,(05):119.[doi:10.11937/bfyy.20223501]
[6]崔晓,丛倩倩,王庆武,等.无机盐、维生素及生长调节剂对卵孢长根菇菌丝的影响[J].北方园艺,2024,(9):110.[doi:10.11937/bfyy.20233497]
CUI Xiao,CONG Qianqian,WANG Qingwu,et al.Effects of Inorganics Salt，Vitamin and Phytohormone on the Mycelial Growth of Oudemansiella raphanipes[J].Northern Horticulture,2024,(05):110.[doi:10.11937/bfyy.20233497]
备注/Memo
第一作者简介：张焱珍（1986-）,女，硕士，助教，研究方向为食用菌资源驯化与遗传。E-mail：421154119@qq.com.