WANG Xian-lei,NING Xue-fei,GAO Xing-wang,et al.Mapping of Powdery Mildew Resistance Gene in Melon PMR5[J].Northern Horticulture,2014,38(21):118-122.
甜瓜PMR5抗白粉病基因的遗传定位
- Title:
- Mapping of Powdery Mildew Resistance Gene in Melon PMR5
- 文章编号:
- 1001-0009（2014）21-0118-05
- Keywords:
- melon; powdery mildew; gene; BSA; SSR
- 分类号:
- Q 943.2
- 文献标志码:
- A
- 摘要:
- 以甜瓜白粉病抗性品种PMR5,感白粉病甜瓜伽师及杂交F1以及BC1、BC2分离群体为试材，接种白粉菌生理小种1，采用抗病遗传分析，并利用BSA（Bulked Segregation Analysis）结合SSR(Simple Sequence Repeat)分子标记方法，研究抗白粉病基因的遗传规律获得与其连锁的分子标记，利用获得的分子标记对抗白粉病基因进行遗传定位。结果表明：BC1中抗病与感病分离比为64∶27，BC2中分离比为37∶27，推测PMR5在BC1中表现2个显性基因，在BC2中表现单显性基因遗传模式。用BC2对抗病基因进行遗传定位发现，一共有11个位于LGII的SSR分子标记与抗白粉病基因连锁，抗病基因位于标记CMGA36和SSR25208之间约104 113 bp。
- Abstract:
- Taking the powdery mildew resistance variety PMR5,susceptible variety Jiashi,F1,BC2 and BC2as materials,and the powdery mildew Podosphaera xanthii race 1 was inoculated.The method of resistance genetic analysis,Bulked Segregation Analysis (BSA) and Simple Sequence Repeat (SSR) markers were adopted to find the inheritance law of powdery mildew resistance,the markers linked to resistance gene,and then construct the genetic map the gene.The results showed that the resistant and susceptible separation ratio was 64∶27 in BC1,and 37∶27 in BC2,suggesting that PMR5 provided two dominant genes in BC1,and single dominant gene in BC2.A total of 11 SSR markers in LGII were found to linked powdery mildew resistance gene,and the gene was located in 104 113 bp between markers CMGA36 and SSR25208.
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备注/Memo
第一作者简介：王贤磊（1981-），男，博士，讲师，研究方向为植物分子遗传。E-mail：wangxianlei2000@163.com.
基金项目：新疆维吾尔自治区自然科学基金资助项目（2012211B03）。
收稿日期：2014-06-10