TANG Ye-gang,CUI Wei.Research on Proliferation Culture Condition of Lilium leucanthum Baker Bulb[J].Northern Horticulture,2014,38(02):112-116.
宜昌百合鳞茎增殖诱导条件研究
- Title:
- Research on Proliferation Culture Condition of Lilium leucanthum Baker Bulb
- 文章编号:
- 1001-0009(2014)02-0112-05
- Keywords:
- Lilium leucanthum Baker; bulb; proliferation; solid culture; suspension culture; culture medium
- 分类号:
- Q 949. 71+8
- 文献标志码:
- A
- 摘要:
- 以宜昌百合种球诱导的鳞茎无菌系为试材，从外植体处理、固体增殖培养基优化及液体增殖培养基优化等3个方面对宜昌百合鳞茎的增殖效果进行了研究。结果表明：对外植体进行创伤处理的鳞茎诱导增殖效果极显著高于不做创伤处理；最佳固体增殖培养基为MS+6-BA 2. 0 mg/L+NAA 0. 3 mg/L+蔗糖30 g/L+琼脂7 g/L，平均增殖倍数可达7. 60倍；最佳液体悬浮增殖培养基为MS+TDZ 0. 1 mg/L+NAA 0. 3 mg/L+蔗糖30 g/L，平均增殖倍数高达10. 86，平均增重倍数可达6. 18；在MS+6-BA 1. 5 mg/L+NAA 0. 4 mg/L+蔗糖30 g/L的培养基下，液体培养的增殖效果极显著高于固体培养的诱导增殖效果。
- Abstract:
- Taking bulb sterile lines induced from the bulb of Lilium leucanthum Baker as experiment material，the bulb proliferation culture condition was studied from three aspects of disposition of explants，solid multiplication medium selection and liquid multiplication medium selection.The results showed that the proliferation effect of wounded bulb was significantly higher than the unwounded explants；the optimal solid multiplication medium was MS+6-BA 2. 0 mg/L+NAA 0. 3 mg/L+sucrose 30 g/L+agar 7 g/L，the average proliferation ratio was up to 7. 60；the best liquid multiplication medium was MS+TDZ 0. 1 mg/L+NAA 0. 3 mg/L+sucrose 30 g/L，the average multiplication ratio was up to 10. 86，and the average weight multiple was up to 6. 18；the effect of multiplication in the suspension culture medium of MS+6-BA 1. 5 mg/L+NAA 0. 4 mg/L+sucrose 30 g/L was significantly higher than the effect of multiplication in the solid medium.
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备注/Memo
第一作者简介：唐业刚（1979-），男，湖北武汉人，硕士，讲师，研究方向为细胞工程。E-mail:tyang126@126.com.