WU Yue-hong.Comparative Study on Antibacterial Effect About Plant Extracts of Six Species of Artemisia L. Plants[J].Northern Horticulture,2014,38(19):121-125.
六种蒿属植物提取物抑菌效果的比较研究
- Title:
- Comparative Study on Antibacterial Effect About Plant Extracts of Six Species of Artemisia L. Plants
- 文章编号:
- 1001-0009(2014)19-0121-05
- 关键词:
- 植物提取物; 抑菌作用; 最小抑菌浓度(MIC)
- 分类号:
- R 284.2
- 文献标志码:
- A
- 摘要:
- 以黄花蒿、艾蒿、茵陈蒿、大籽蒿、蒙古蒿、林地蒿6种菊科蒿属植物的乙醇提取物为试材,采用滤纸片扩散法和液体悬浮培养法,研究6种植物乙醇提取物对引起植物细菌性病害的丁香假单胞菌、根癌土壤杆菌、马铃薯环腐病菌的抑菌效果的影响和最小抑菌浓度(MIC)。结果表明:6种蒿属植物的乙醇提取物均表现为对供试细菌有一定的抑菌作用,其中艾蒿、黄花蒿的抑菌作用最强。不同植物的乙醇提取物对不同细菌的最小抑菌浓度(MIC)则表现较大差异。
- Abstract:
- Taking the ethanol extracts of six species(Artemisia annua,Artemisia argyi,Artemisia capillaries,Artemisia sieversiana,Artemisia mongolica Fisch.et Bes,Artemisia sylvatica) of Artemisia L. plants as experimental materials.Filter paper dispersion method and the liquid suspension culture method were used to determine antibacterial effect andthe minimum inhibitory concentrations(MIC) of the ethanol extracts of six species of Artemisia L. plants on Pseudomonas syringae,A.tumefaciens,Clavibacter michiganensis subsp.sepedonicus.The results showed that the ethanol extracts of six species of Artemisia L. plants had certain bacteriostatic activity on the tested bacteria.Testing of bacteriostasis showed that the strongest bacteriostasis was the plant extracts of Artemisia argyi followed by Artemisia annua.The MIC of ethanol extracts from different plants showed significant difference.
参考文献/References:
[1]张应烙,尹彩萍,赖伟明,等.井冈山47种植物提取物对几种病原菌的生物活性[J].江苏农业科学,2005(4):51-53.
[2]白洁,王艳梅,赵强.天山花楸全株抑菌作用的比较[J].食品科学,2007,28(9):125-127.
[3]张佳,王莹,张峰,等.滤纸片法测定黄花蒿提取物对霉菌的抑制活性[J].湖北农业科学,2009,48(5):1153-1154. [4]耿剑锋,黑田克利,田中一久.洋葱油和大蒜提取物对灰霉菌的作用效果[J].中国蔬菜,2008(5):20-22.
[5]孟祥东,傅俊范,严雪瑞.灰霉病菌生物防治研究进展[J].沈阳农业大学学报,2003,34(6):472-475.
[6]张佳,王莹,张峰,等.滤纸片法测定黄花蒿提取物对霉菌的抑制活性[J].湖北农业科学,2009,48(5):1153-1154. [7]王兴全,张新虎,杨顺义,等.苍耳提取物对番茄灰霉病菌抑制机理的研究[J].甘肃农业大学学报,2008,43(6):107-110.
[8]李永刚,文景芝,郝中娜.植物源杀菌剂的研究现状与展望[J].东北农业大学学报,2002,33(2):198-202.
[9]何衍彪,詹儒林,赵艳龙.植物源农药的研究与应用[J].热带农业科学,2004,24(3):48-56.
[10]张金林,常志卷,李维宽.植物性杀菌剂研究进展[J].河北农业大学学报,1998,21(3):112-115.
[11]Kawchuk L M,Hutchison L J,Verhaeghe C A,et al.Isolation of the β-tubulin gene and characterization of thiabendazole resistance in Gibberella pulicaris[J].Can J Plant Pathol,2002,24:233-238.
[12]Edlind T,Henry K W,Metera K A,et al.Aspergillus fumigatus CYP51 sequence:potential basis for fluconazole resistance[J].Med Mycol,2001,39:299-302.
[13]沈萍,范秀容,李广武.微生物学实验[M].北京:高等教育出版社,2001:49-74.
[14]卢婷,朴香淑.绿萝提取物抗氧化活性的研究进展[J].中国畜牧杂志,2009,45(15):57-60.
[15]程晓丽,肖晴,刘明星,等.绿萝乙醇提取物对小鼠抵御伤寒沙门菌感染能力的研究[J].中国药师,2009,12(4):435-438.
[16]尹乐乐,曾耀英,侯会娜.绿萝提取物对脓毒血症小鼠及体内T淋巴细胞影响[J].现代免疫学,2009,29(5):392-396.
[17]胡文静,钱晓萍,涂云霞,等.绿萝乙醇提取物抗肿瘤作用的实验研究[J].南京中医药大学学报,2007,23(6):379-382.
[18]Sierrotzki H,Parisi S,Steinfeld U,et al.Mode of resistance to respiration inhibitors at the cytochrome bc1 enzyme complex of Mycosphaerella fijiensis field isolates[J].Pest Management Sci,2000,56:833-841.
[19]李宏,姜怀春.贯叶绿萝总提取物对致病细菌的抗菌作用[J].广西植物,2007,27(3):466-468.
[20]Takayuki M,Kaori K,Tomohiro O,et al.A two-component histidine kinase of the rice blast fungus is involved in osmotic stress response and fungicide action[J].Fungal Genetics and Biology,2005,42:200-212.
相似文献/References:
[1]周梦娇,万春鹏,陈金印.植物提取物对柑橘采后病害抑制及保鲜效果的研究进展[J].北方园艺,2014,38(02):186.
ZHOU Meng-jiao,WAN Chun-peng,CHEN Jin-yin.Research Progress on Inhibition of Citrus Postharvest Diseases and Preservation Effects of Plant Extraction[J].Northern Horticulture,2014,38(19):186.
[2]张焱珍,肖志新,浦 勇,等.四种植物提取物对烟草赤星病菌抑菌活性研究[J].北方园艺,2014,38(11):108.
ZHANG Yan-zhen,XIAO Zhi-xin,PU Yong,et al.Study on Antifungal Activity of Four Plant Extracts on Alternaria alternata[J].Northern Horticulture,2014,38(19):108.
[3]于志会,杨波,戚继忠.吉林市主要绿化植物对两种致病细菌抑制作用[J].北方园艺,2012,36(01):108.
[J].Northern Horticulture,2012,36(19):108.
[4]张新燕,刘海光,邱广艳,等.不同药剂对平菇绿色木霉的防治研究[J].北方园艺,2012,36(01):151.
ZHANG Xin-yan,LIU Hai-guang,QIU Guang-yan,et al.Study on Control of Different Fungicides to Trichoderma viride on Pleurotus ostreatus[J].Northern Horticulture,2012,36(19):151.
[5]郭彩霞,迟晓红,谢克虎.不同杀菌剂对万年青炭疽病的室内抑菌作用及田间药效研究[J].北方园艺,2012,36(16):121.
GUO Cai-xia,CHI Xiao-hong,XIE Ke-hu.Study on Field Efficaney of Different Fungicides on Evergreen Anthrax Pathogens[J].Northern Horticulture,2012,36(19):121.
[6]暴增海,石桐磊.食用菌的抑菌作用[J].北方园艺,2012,36(18):174.
BAO Zeng-hai,SHI Tong-lei.Anti-Bacterial Functions of Edible Fungus[J].Northern Horticulture,2012,36(19):174.
[7]黄娇.峨眉山四种菖蒲挥发油及水提取液的抑菌作用比较[J].北方园艺,2014,38(21):67.
HUANG Jiao.The Antibacterial Activity of Volatile Oils and Water Extracts from Four Kinds of Acorus in Emei Mountain[J].Northern Horticulture,2014,38(19):67.
[8]高婷婷,余风华,谭勇,等.三种阿魏根提取物的体外抑菌作用研究[J].北方园艺,2013,37(24):156.
GAO Ting-ting,YU Feng-hua,TAN Yong,et al.Study on Antimicrobial Activity in vitro of Extracts from Three Species of Ferula Root[J].Northern Horticulture,2013,37(19):156.
[9]贺润丽,樊杰,平莉莉,等.不同植物提取物对款冬蚜虫杀虫活性研究[J].北方园艺,2015,39(16):119.[doi:10.11937/bfyy.201516028]
HE Runli,FAN Jie,PING Lili,et al.Insecticidal Activity of the Extracts From Different Plants Against Coltsfoot Aphids[J].Northern Horticulture,2015,39(19):119.[doi:10.11937/bfyy.201516028]
[10]和 玉 华,杨 婧,谢 雯 颖,等.微量点滴法测定植物提取物对绣线菊蚜的触杀作用[J].北方园艺,2010,34(04):0.[doi:10.11937/bfyy.201004060]
HE Yu - hua,YANG Jing,XIE Wen- ying,et al.The Contact Toxicity of Plant Extracts on Aphiscitricola by Micro - drip[J].Northern Horticulture,2010,34(19):0.[doi:10.11937/bfyy.201004060]
[11]刘胜贵,李路,左清清,等.悬钩子属植物提取物的抑菌效果及抗氧化性研究[J].北方园艺,2014,38(06):128.
LIU Sheng-gui,LI Lu,ZUO Qing-qing,et al.Study on Antimicrobial and Antioxidant Activity of the Extract of Rubus Spp.[J].Northern Horticulture,2014,38(19):128.
备注/Memo
作者简介:武月红(1975-),女,硕士,副教授,研究方向为植物生理学与微生物学。E-mail:redmaylyy@126.com.
收稿日期:2014-04-21