[1]张东向,赵静,刘丽杰,等.黄芩悬浮细胞培养过程中黄芩苷含量与苯丙氨酸解氨酶活性分析[J].北方园艺,2014,38(16):155-157,158.
　ZHANG Dong-xiang,ZHAO Jing,LIU Li-jie,et al.Analysis of Content of Baicalin and Activity of Phenylalanine Ammonia Lyase from Cell Suspension Cultures of Scutellaria baicalensis[J].Northern Horticulture,2014,38(16):155-157,158.
点击复制

黄芩悬浮细胞培养过程中黄芩苷含量与苯丙氨酸解氨酶活性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年16期 页码: 155-157,158 栏目: 出版日期: 2014-08-30
Title:
Analysis of Content of Baicalin and Activity of Phenylalanine Ammonia Lyase from Cell Suspension Cultures of Scutellaria baicalensis
文章编号:
1001-0009(2014)16-0155-04
作者:
张东向; 赵静; 刘丽杰; 焦战战; 张令昂; 毕宇
齐齐哈尔大学 生命科学与农林学院，黑龙江 齐齐哈尔 161006
Author(s):
ZHANG Dong-xiang; ZHAO Jing; LIU Li-jie; JIAO Zhan-zhan; ZHANG Ling-ang; BI Yu
College of Life Science and Agro-forestry,Qiqihar University,Qiqihar,Heilongjiang 161006
关键词:
黄芩; 悬浮细胞培养; 黄芩苷; PAL; 均匀设计
Keywords:
Scutellaria baicalensis; suspension culture; baicalin; PAL; uniform design
分类号:
R284
文献标志码:
A
摘要:
以黄芩悬浮细胞为试材，研究了悬浮培养过程中黄芩苷含量与苯丙氨酸解氨酶活性变化，采用均匀设计法分析了不同激素配比对黄芩生物量积累的影响。结果表明:黄芩细胞悬浮系生长呈较典型的“S形”曲线，黄芩苷变化与生物量积累基本一致；在黄芩悬浮培养周期中，苯丙氨酸解氨酶的活性强弱与黄芩苷的积累有一定相关性，培养第12天时活性最强。经均匀设计实验法得到最适宜黄芩悬浮培养生物量积累的激素配比为MS培养基附加6-BA 0.75 mg/L，NAA 0.5 mg/L，KT 1.5 mg/L。
Abstract:
Using cell suspension cultures of Scutellaria baicalensis as materials,the change of baicalin contents and activities of phenylalanine ammonialyase (PAL) from cell suspension cultures of Scutellaria baicalensis were analyzed in this paper.The effects of plant hormone proportions on biomass accumulation of the cell suspension cultures were studied by uniform design.The results showed that the growth curves of the cell suspension cultures exhibited ‘S’ curve,and biomass accumulation of the cell suspension cultures matched with change of baicalin contents.There was correlation between PAL activities and change of baicalin contents.The most suitable hormone ratio to biomass accumulation of the suspension cultures was MS medium added with 6-BA 0.75 mg/L,NAA 0.5 mg/L,KT 1.5 mg/L by uniform design method.

参考文献/References:

［1］中国华人民共和国药典委员会.中华人民共和国药典［M］.北京:中国医药科技出版社,2010.

［2］张宽朝,金青,蔡永萍,等.苯丙氨酸解氨酶与其在重要此生产物调控中的作用研究进展［J］.中国农学通报,2008（12）:59-62.
［3］王敬文,薛应龙.植物苯丙氨酸解氨酶的研究-植物激素对甘薯块根植物苯丙氨酸解氨酶和肉桂酸羟化酶活性变化机器伴随性的影响［J］.植物生理学报,1981,7(4):173-179.
［4］文芳,苏湘鄂,梅兴国.红豆杉细胞悬浮培养系统激素优化的研究［J］.湖北农学院学报,2010,20(3):232-235.
［5］姚娜,安新民,杨凯,等.毛白杨悬浮细胞系的建立及再生植株的获得［J］.植物生理学通讯,2010,46(11):1114-1118.
［6］李蕤,谭晓芳,陈群,等.霍山石斛细胞悬浮培养及条件优化［J］.中草药,2011,42(2):358-361.
［7］张坚,高文远,王娟,等.杠柳细胞悬浮培养体系的建立及有效成分杠柳毒苷和4-甲氧基水杨醛含量的测定［J］.天津中医药,2010,27(2):163-165.
［8］王健,郭政宏,刘海军,等.魔芋细胞悬浮培养生产葡甘露聚糖的研究［J］.安徽农业科学,2010,38(27):14836-14837.
［9］王文星,安琪,汪莹,等.瑞香狼毒细胞悬浮培养及黄酮积累的研究［J］.草业学报,2010，19(6):132-138.
［10］方开泰.均匀设计与均匀设计表［M］.北京:科学出版社,1994:69-97.
［11］任民红,上官新晨,陈继光,等.几种理化因子对青钱柳悬浮细胞生长的影响［J］.西北农业学报,2012,21(5):179-184.
［12］王莉,史玲玲,刘玉军,等.不同光质对长鞭红景天悬浮细胞生长及苯丙氨酸解氨酶活性的影响［J］.林业科学,2007,43(6):52-56.

相似文献/References:

[1]焦战战,张东向,赵静,等.黄芩愈伤组织中黄芩苷与过氧化物酶活性相关性分析[J].北方园艺,2013,37(13):108.
　JIAO Zhan-zhan,ZHANG Dong-xiang,ZHAO Jing,et al.Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi[J].Northern Horticulture,2013,37(16):108.
[2]雷桅,税晓容,胡侃.黄芩苷生物合成途径与生物技术研究进展[J].北方园艺,2014,38(22):185.
　LEI Wei,SHUI Xiao-rong,HU Kan.Study Advance on Biosynthesis Pathway and Biotechnology of Baicalin[J].Northern Horticulture,2014,38(16):185.
[3]韩立军,崔爱辉,邵玺文.种植密度对黄芩生育性状和产量的影响[J].北方园艺,2015,39(02):141.[doi:10.11937/bfyy.201502041]
　HAN Li-jun,CUI Ai-hui,SHAO Xi-wen.Effect of Planting Density on Growing Development Traits and Yield of Scutellaria baicalensis[J].Northern Horticulture,2015,39(16):141.[doi:10.11937/bfyy.201502041]
[4]刘丽杰,林立东,张东向.黄芩单细胞克隆愈伤组织酯酶同工酶及过氧化物同工酶酶谱分析[J].北方园艺,2015,39(07):85.[doi:10.11937/bfyy.201507026]
　LIU Li-jie,LIN Li-dong,ZHANG Dong-xiang.Analysis on Esterase Isozemes and Peroxidase Isozymes in Singlecell Clone of Scutellaria Callus[J].Northern Horticulture,2015,39(16):85.[doi:10.11937/bfyy.201507026]
[5]刘乐乐,刘奇志.间作黄芩的仁用杏园主要害虫发生动态特征分析[J].北方园艺,2015,39(23):128.[doi:10.11937/bfyy.201523036]
　LIU Lele,LIU Qizhi.Dynamics of the Major Pests in Kernel Apricot Orchards Intercropping With Scutellaria baicalensis[J].Northern Horticulture,2015,39(16):128.[doi:10.11937/bfyy.201523036]
[6]姜明亮,王景然,全雪丽,等.黄芩总黄酮含量的累积规律研究[J].北方园艺,2016,40(01):134.[doi:10.11937/bfyy.201601035]
　JIANG Mingliang,WANG Jingran,QUAN Xueli,et al.Study on Accumulation Rule of Total Flavonoids Content of Scutellaria baicalensis[J].Northern Horticulture,2016,40(16):134.[doi:10.11937/bfyy.201601035]
[7]陈智坤,邓娴,王峰伟,等.蘑菇渣堆肥滤液对盐胁迫下黄芩种子萌发和幼苗生长的影响[J].北方园艺,2016,40(18):149.[doi:10.11937/bfyy.201618037]
　CHEN Zhikun,DENG Xian,WANG Fengwei,et al.Effect of Spent Mushroom Compost Leachate on Seed Germination and Seeding Growth of Scutellaria baicalensis Georgi Under Salt Stress[J].Northern Horticulture,2016,40(16):149.[doi:10.11937/bfyy.201618037]
[8]李 富 雄,张 东 向,李 善 文.黄芩同源四倍体的诱导及细胞形态学观察[J].北方园艺,2009,33(03):0.[doi:10.11937/bfyy.200903044]
　LI Fu -xiong,ZHANG Dong -xiang,LI Shan -wen.The Inducing of Autotetraploid of Scutellaria baicalensis Georgi[J].Northern Horticulture,2009,33(16):0.[doi:10.11937/bfyy.200903044]
[9]张 东 向,张 　 磊,王 　 蕊.黄芩过氧化物酶同工酶几种酶液提取方法的比较[J].北方园艺,2008,32(06):0.[doi:10.11937/bfyy.200806012]
　ZHANG Dong -xiang,ZHANG Lei,WANG Rui.Comparison on Several Extraction Methods of Enzyme Solution of Peroxidase and Isoenzyme of Scutellaria baicalensis Georgi[J].Northern Horticulture,2008,32(16):0.[doi:10.11937/bfyy.200806012]
[10]石 春 红,郑 有 飞,李 红 双,等.黄芩中天然防晒剂的索氏提取工艺研究[J].北方园艺,2008,32(12):0.[doi:10.11937/bfyy.2008012073]
　SHI Chun- hong,ZHENG You - fei,LI Hong- shuang,et al.Research on the Extraction of Natural Sunscreen from the Radix Scutellariae by Soxhlet Extraction[J].Northern Horticulture,2008,32(16):0.[doi:10.11937/bfyy.2008012073]

备注/Memo

第一作者简介：张东向（1963-），男，硕士，教授，硕士生导师，现主要从事植物生理及植物细胞工程等研究工作。E-mail：zhangdx1019@163.com.

基金项目：黑龙江省自然科学基金资助项目（C201013）。

更新日期/Last Update: 2014-09-26